LOCUS       pcDNA3-ER-GCaMP3.gb.xdna    5980 bp      DNA     circular   4/16/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     sig_peptide     32..112
                     /label=ER-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             128..145
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1028..1468
                     /label=CaM
                     /SerialCloner_Color=&hFF6666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3819..3839)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1533..1550)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4207..4835)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(3845..3867)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(4987..5646)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2688..3479
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5886..5914)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(3872..3901)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2469..2546
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      1573..1800
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    230..292
                     /label=M13 peptide
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             128..145
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             299..568
                     /label=GFP-C
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             587..1021
                     /label=GFP-N
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 aagcttCAGT TGTCAGGAAG AGAAGGCTCC CATGGACCCT GTGGTGGTGC TGGGGCTCTG 
       61 TCTCTCCTGT TTGCTTCTCC TTTCACTCTG GAAACAGAGC TATGGGGGAG GggatcccAT 
      121 GGGTTCTCAT CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG 
      181 TCGGGATCTG TACGACGATG ACGATAAGGA TCTCGCCACC ATGGTCGACT CATCACGTCG 
      241 TAAGTGGAAT AAGACAGGTC ACGCAGTCAG AGCTATAGGT CGGCTGAGCT CACTCGAGAA 
      301 CGTCTATATC AaGGCCGACA AGCAGAAGAA CGGCATCAAG GCGAACTTCA AGATCCGCCA 
      361 CAACATCGAG GACGGCGGCG TGCAGCTCGC CTACCACTAC CAGCAGAACA CCCCCATCGG 
      421 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC gtgCAGTCCA AACTTTCGAA 
      481 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT 
      541 CACTCTCGGC ATGGACGAGC TGTACAAGGG CGGTACCGGA GGGAGCATGG TGAGCAAGGG 
      601 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG 
      661 CCACAAGTTC AGCGTGTCCG GCGAGGGTGA GGGCGATGCC ACCTACGGCA AGCTGACCCT 
      721 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 
      781 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT 
      841 CAAGTCCGCC ATGCCCGAAG GCTACATCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG 
      901 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 
      961 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA 
     1021 CACGCGTGAC CAACTGACTG AAGAGCAGAT CGCAGAATTT AAAGAGGCTT TCTCCCTATT 
     1081 TGACAAGGAC GGGGATGGGA CAATAACAAC CAAGGAGCTG GGGACGGTGA TGCGGTCTCT 
     1141 GGGGCAGAAC CCCACAGAAG CAGAGCTGCA GGACATGATC AATGAAGTAG ATGCCGACGG 
     1201 TgacGGCACA ATCGACTTCC CTGAGTTCCT GACAATGATG GCAAGAAAAA TGAAAGACAC 
     1261 AGACAGTGAA GAAGAAATTA GAGAAGCGTT CCGTGTGTTT GATAAGGATG GCAATGGCTA 
     1321 CATCAGTGCA GCAGAGCTTC GCCACGTGAT GACAAACCTT GGAGAGAAGT TAACAGATGA 
     1381 AGAGGTTGAT GAAATGATCA GGGAAGCAGA CATCGATGGG GATGGTCAGG TAAACTACGA 
     1441 AGAGTTTGTA CAAATGATGA CAGCGAAGTA AgaattcTGC AGATATCCAT CACACTGGCG 
     1501 GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTctatagtg tcacctaaat gctagagctc 
     1561 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 
     1621 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 
     1681 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 
     1741 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 
     1801 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 
     1861 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     1921 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     1981 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 
     2041 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     2101 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     2161 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 
     2221 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 
     2281 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta 
     2341 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 
     2401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 
     2461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     2521 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 
     2581 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 
     2641 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 
     2701 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 
     2761 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 
     2821 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 
     2881 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 
     2941 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 
     3001 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 
     3061 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 
     3121 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 
     3181 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 
     3241 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 
     3301 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 
     3361 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 
     3421 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 
     3481 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 
     3541 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 
     3601 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 
     3661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     3721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     3781 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 
     3841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     3901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     3961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     4021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     4081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     4141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     4201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     4261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     4321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     4381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 
     4441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     4501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     4561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     4621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     4681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     4741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     4801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     4861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     4921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     4981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     5041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     5101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     5161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     5221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     5281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     5341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     5401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     5461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     5521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     5581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     5641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     5701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     5761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     5821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     5881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     5941 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
//