LOCUS       pKESK22                3952 bp    DNA     circular      6-JUN-2014
DEFINITION  lacI-q Ptac MCS in pACYC KanR.
SOURCE      
  ORGANISM  
COMMENT     AUTHOREML|schnetz@uni-koeln.de|
FEATURES             Location/Qualifiers
     promoter        14..50
                     /vntifkey="30"
                     /label=P-lacI-q
                     /note="lacI-q promoter"
     CDS             78..1160
                     /vntifkey="4"
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1153..1173
                     /vntifkey="31"
                     /label=lacO3
                     /note="lac operator"
     promoter        1209..1236
                     /vntifkey="30"
                     /label=tac
     protein_bind    1243..1263
                     /vntifkey="31"
                     /label=lacO
                     /note="lac operator"
     promoter        complement(3800..3843)
                     /vntifkey="30"
                     /label=P-primer
                     /note="primer promoter p15A ori"
     promoter        3664..3699
                     /vntifkey="30"
                     /label=RNAI-P
                     /note="RNA-I promoter p15A ori"
     repeat_unit     complement(1601..1828)
                     /vntifkey="35"
                     /label=TN903_INV_RPT
                     /note=" Tn903 inverted repeat "
     CDS             complement(1888..2703)
                     /vntifkey="4"
                     /label=neo
                     /note="kanR kanamycin resistance, Tn903 type I"
     repeat_unit     complement(2809..3036)
                     /vntifkey="35"
                     /label=TN903_INV_RPT
                     /note="Tn903 inverted repeat "
     rep_origin      complement(3031..3860)
                     /vntifkey="33"
                     /label=P15A_ORI
                     /note=" p15A origin of replication "
     repeat_unit     complement(3861..3898)
                     /vntifkey="35"
                     /label=TN3_INV_RPT
                     /note=" Tn3 inverted repeat "
BASE COUNT      999 a      1017 c       977 g       959 t 
ORIGIN
        1 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 
       61 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 
      121 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 
      181 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 
      241 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 
      301 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 
      361 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 
      421 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 
      481 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 
      541 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 
      601 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 
      661 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 
      721 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 
      781 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 
      841 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 
      901 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 
      961 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 
     1021 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 
     1081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     1141 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcaa atattctgaa 
     1201 atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca 
     1261 atttcacaca ggaaacagaa ttcgctagcc cgggtctaga ggatcctccg gcgttcagcc 
     1321 tgtgccacag ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc 
     1381 ccgtcgtcaa taaaccgaac cgctacaccc tgagcatcaa actcttttat cagttggatc 
     1441 atgtcggcgg tgtcgcggcc aagacggtcg agcttcttca ccagaatgac atcaccttcc 
     1501 tccaccttca tcctcagcaa atccagccct tcccgatctg ttgaactgcc ggatgccttg 
     1561 tcggtaaaga tgcggttagc ttttacccct gcatctttga gcgctgaggt ctgcctcgtg 
     1621 aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga 
     1681 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt 
     1741 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag 
     1801 caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca 
     1861 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 
     1921 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 
     1981 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 
     2041 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 
     2101 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat 
     2161 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc 
     2221 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt 
     2281 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc 
     2341 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg 
     2401 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg 
     2461 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc 
     2521 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg 
     2581 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc 
     2641 agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct 
     2701 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat 
     2761 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat 
     2821 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg 
     2881 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat 
     2941 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc 
     3001 agcaacacct tcttcacgag gcagacctca gcgctagcgg agtgtatact ggcttactat 
     3061 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac 
     3121 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc 
     3181 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc 
     3241 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt 
     3301 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg 
     3361 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc 
     3421 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca 
     3481 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg 
     3541 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     3601 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta 
     3661 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct 
     3721 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg 
     3781 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct 
     3841 caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     3901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttggt cg 
//
