LOCUS       pcDNA3-TORCAR1(T-A).gb.xdna    7225 bp      DNA     circular   7/16/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    914..1630
                     /label=CER
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1640..1993
                     /label=4EBP1(T/A)
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTAPGGTLFSTAPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDIEL" \ 
     gene            2000..2713
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5064..5084)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2778..2795)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5452..6080)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5090..5112)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6232..6891)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3933..4724
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7131..7159)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5117..5146)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3714..3791
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2818..3045
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcGGAT CCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT 
      961 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAGGTTCAGC GTGTCCGGCG AGGGCGAGGG 
     1021 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT 
     1081 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCG CCCGCTACCC 
     1141 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA 
     1201 GCGTACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA 
     1261 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA 
     1321 CATCCTGGGG CACAAGCTGG AGTACAACGC CATCAGTGAC AACGTCTATA TCACCGCCGA 
     1381 CAAGCAGAAG AACGGCATCA AGGCCCACTT CAAGATCCGC CACAACATCG AGGACGGCAG 
     1441 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT 
     1501 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG 
     1561 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA 
     1621 GCTGTACAAG CGCATGCATA TGTCCGGGGG CAGCAGCTGC AGCCAGACCC CAAGCCGGGC 
     1681 CATCCCCGCC ACTCGCCGGG TGGTGCTCGG CGACGGCGTG CAGCTCCCGC CCGGGGACTA 
     1741 CAGCACGGCC CCCGGCGGCA CGCTCTTCAG CACCGCCCCG GGAGGTACCA GGATCATCTA 
     1801 TGACCGGAAA TTCCTGATGG AGTGTCGGAA CTCACCTGTG ACCAAAACAC CCCCAAGGGA 
     1861 TCTGCCCACC ATTCCGGGGG TCACCAGCCC TTCCAGTGAT GAGCCCCCCA TGGAAGCCAG 
     1921 CCAGAGCCAC CTGCGCAATA GCCCAGAAGA TAAGCGGGCG GGCGGTGAAG AGTCACAGTT 
     1981 TGAGATGGAC ATTGAGCTCT CTAAAGGTGA AGAATTATTC ACTGGTGTTG TCCCAATTTT 
     2041 GGTTGAATTA GATGGTGATG TTAATGGTCA CAAATTTTCT GTCTCCGGTG AAGGTGAAGG 
     2101 TGATGCTACG TACGGTAAAT TGACCTTAAA ATTACTCTGT ACTACTGGTA AATTGCCAGT 
     2161 TCCATGGCCA ACCTTAGTCA CTACTTTAGG TTATGGTGTT CAATGTTTTG CTAGATACCC 
     2221 AGATCATATG AAACAACATG ACTTTTTCAA GTCTGCCATG CCAGAAGGTT ATGTTCAAGA 
     2281 AAGAACTATT TTTTTCAAAG ATGACGGTAA CTACAAGACC AGAGCTGAAG TCAAGTTTGA 
     2341 AGGTGATACC TTAGTTAATA GAATCGAATT AAAAGGTATT GATTTTAAAG AAGATGGTAA 
     2401 CATTTTAGGT CACAAATTGG AATACAACTA TAACTCTCAC AATGTTTACA TCACTGCTGA 
     2461 CAAACAAAAG AATGGTATCA AAGCTAACTT CAAAATTAGA CACAACATTG AAGATGGTGG 
     2521 TGTTCAATTA GCTGACCATT ATCAACAAAA TACTCCAATT GGTGATGGTC CAGTCTTGTT 
     2581 ACCAGACAAC CATTACTTAT CCTATCAATC TGCCTTATTC AAAGATCCAA ACGAAAAGAG 
     2641 AGACCACATG GTCTTGTTAG AATTTTTGAC TGCTGCTGGT ATTACCGAGG GTATGAATGA 
     2701 ATTGTACAAA TAGTAAGAAT TCtgcagata tccatcacac tggcggccgc tcgagcatgc 
     2761 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga 
     2821 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 
     2881 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 
     2941 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 
     3001 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 
     3061 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 
     3121 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     3181 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     3241 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     3301 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     3361 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     3421 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt 
     3481 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 
     3541 gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc 
     3601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     3661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     3721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     3781 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 
     3841 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 
     3901 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 
     3961 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 
     4021 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 
     4081 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 
     4141 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 
     4201 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 
     4261 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 
     4321 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 
     4381 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 
     4441 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 
     4501 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 
     4561 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 
     4621 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 
     4681 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 
     4741 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 
     4801 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 
     4861 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 
     4921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     4981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 
     5041 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     5101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     5161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     5221 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     5281 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     5341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     5401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     5461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     5521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     5581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     5641 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 
     5701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     5761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     5821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     5881 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     5941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     6001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     6061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     6121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     6181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     6241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     6301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     6361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     6421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     6481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     6541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     6601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     6661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     6721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     6781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     6841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     6901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     6961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     7021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     7081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     7141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     7201 tccccgaaaa gtgccacctg acgtc
//