LOCUS       pc'_Lyso-TORCAR1.xdna    8493 bp      DNA     circular   4/8/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             2908..3261
                     /label=4EBP1
                     /SerialCloner_Color=&h666666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI" \ 
     misc_feature    2182..2898
                     /label=CER
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(6332..6352)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4046..4063)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(6720..7348)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(6358..6380)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(7500..8159)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             5201..5992
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(8399..8427)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6385..6414)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4982..5059
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      4086..4313
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3268..3978
                     /label=YPet
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNELYK" \ 
     CDS             907..2172
                     /label=LAMP1
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="KLAAATMAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIVLIAYLVGRKRSHAGYQTIGGGGG" \ 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAa gcttgcggcc 
      901 gccaccatgg cggcccccgg cagcgcccgg cgacccctgc tgctgctact gctgttgctg 
      961 ctgctcggcc tcatgcattg tgcgtcagca gcaatgttta tggtgaaaaa tggcaacggg 
     1021 accgcgtgca taatggccaa cttctctgct gccttctcag tgaactacga caccaagagt 
     1081 ggccctaaga acatgacctt tgacctgcca tcagatgcca cagtggtgct caaccgcagc 
     1141 tcctgtggaa aagagaacac ttctgacccc agtctcgtga ttgcttttgg aagaggacat 
     1201 acactcactc tcaatttcac gagaaatgca acacgttaca gcgtccagct catgagtttt 
     1261 gtttataact tgtcagacac acaccttttc cccaatgcga gctccaaaga aatcaagact 
     1321 gtggaatcta taactgacat cagggcagat atagataaaa aatacagatg tgttagtggc 
     1381 acccaggtcc acatgaacaa cgtgaccgta acgctccatg atgccaccat ccaggcgtac 
     1441 ctttccaaca gcagcttcag caggggagag acacgctgtg aacaagacag gccttcccca 
     1501 accacagcgc cccctgcgcc acccagcccc tcgccctcac ccgtgcccaa gagcccctct 
     1561 gtggacaagt acaacgtgag cggcaccaac gggacctgcc tgctggccag catggggctg 
     1621 cagctgaacc tcacctatga gaggaaggac aacacgacgg tgacaaggct tctcaacatc 
     1681 aaccccaaca agacctcggc cagcgggagc tgcggcgccc acctggtgac tctggagctg 
     1741 cacagcgagg gcaccaccgt cctgctcttc cagttcggga tgaatgcaag ttctagccgg 
     1801 tttttcctac aaggaatcca gttgaataca attcttcctg acgccagaga ccctgccttt 
     1861 aaagctgcca acggctccct gcgagcgctg caggccacag tcggcaattc ctacaagtgc 
     1921 aacgcggagg agcacgtccg tgtcacgaag gcgttttcag tcaatatatt caaagtgtgg 
     1981 gtccaggctt tcaaggtgga aggtggccag tttggctctg tggaggagtg tctgctggac 
     2041 gagaacagca tgctgatccc catcgctgtg ggtggtgccc tggcggggct ggtcctcatc 
     2101 gtcctcatcg cctacctcgt cggcaggaag aggagtcacg caggctacca gactatcGGT 
     2161 GGTGGCGGTG GTGCGGATCC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG 
     2221 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA GGTTCAGCGT GTCCGGCGAG 
     2281 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG 
     2341 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT GGGGCGTGCA GTGCTTCGCC 
     2401 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC 
     2461 GTCCAGGAGC GTACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG 
     2521 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG 
     2581 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACGCCA TCAGTGACAA CGTCTATATC 
     2641 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCCACTTCA AGATCCGCCA CAACATCGAG 
     2701 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 
     2761 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 
     2821 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 
     2881 ATGGACGAGC TGTACAAGCG CATGCATATG TCCGGGGGCA GCAGCTGCAG CCAGACCCCA 
     2941 AGCCGGGCCA TCCCCGCCAC TCGCCGGGTG GTGCTCGGCG ACGGCGTGCA GCTCCCGCCC 
     3001 GGGGACTACA GCACGACCCC CGGCGGCACG CTCTTCAGCA CCACCCCGGG AGGTACCAGG 
     3061 ATCATCTATG ACCGGAAATT CCTGATGGAG TGTCGGAACT CACCTGTGAC CAAAACACCC 
     3121 CCAAGGGATC TGCCCACCAT TCCGGGGGTC ACCAGCCCTT CCAGTGATGA GCCCCCCATG 
     3181 GAAGCCAGCC AGAGCCACCT GCGCAATAGC CCAGAAGATA AGCGGGCGGG CGGTGAAGAG 
     3241 TCACAGTTTG AGATGGACAT TGAGCTCTCT AAAGGTGAAG AATTATTCAC TGGTGTTGTC 
     3301 CCAATTTTGG TTGAATTAGA TGGTGATGTT AATGGTCACA AATTTTCTGT CTCCGGTGAA 
     3361 GGTGAAGGTG ATGCTACGTA CGGTAAATTG ACCTTAAAAT TACTCTGTAC TACTGGTAAA 
     3421 TTGCCAGTTC CATGGCCAAC CTTAGTCACT ACTTTAGGTT ATGGTGTTCA ATGTTTTGCT 
     3481 AGATACCCAG ATCATATGAA ACAACATGAC TTTTTCAAGT CTGCCATGCC AGAAGGTTAT 
     3541 GTTCAAGAAA GAACTATTTT TTTCAAAGAT GACGGTAACT ACAAGACCAG AGCTGAAGTC 
     3601 AAGTTTGAAG GTGATACCTT AGTTAATAGA ATCGAATTAA AAGGTATTGA TTTTAAAGAA 
     3661 GATGGTAACA TTTTAGGTCA CAAATTGGAA TACAACTATA ACTCTCACAA TGTTTACATC 
     3721 ACTGCTGACA AACAAAAGAA TGGTATCAAA GCTAACTTCA AAATTAGACA CAACATTGAA 
     3781 GATGGTGGTG TTCAATTAGC TGACCATTAT CAACAAAATA CTCCAATTGG TGATGGTCCA 
     3841 GTCTTGTTAC CAGACAACCA TTACTTATCC TATCAATCTG CCTTATTCAA AGATCCAAAC 
     3901 GAAAAGAGAG ACCACATGGT CTTGTTAGAA TTTTTGACTG CTGCTGGTAT TACCGAGGGT 
     3961 ATGAATGAAT TGTACAAATA GTAAGAATTC TGCAGATATC CATCACACTG GCGGCCGCTC 
     4021 GAGCATGCAT CTAGAGGGCC CTATTctata gtgtcaccta aatgctagag ctcgctgatc 
     4081 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 
     4141 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 
     4201 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 
     4261 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 
     4321 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 
     4381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     4441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     4501 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     4561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     4621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     4681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 
     4741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 
     4801 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 
     4861 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     4921 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     4981 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     5041 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 
     5101 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 
     5161 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 
     5221 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     5281 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     5341 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 
     5401 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     5461 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     5521 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     5581 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     5641 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     5701 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 
     5761 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     5821 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     5881 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     5941 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     6001 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 
     6061 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 
     6121 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 
     6181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     6241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 
     6301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
     6361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
     6421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     6481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     6541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     6901 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     7801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     7861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     7921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     7981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     8041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     8101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     8161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     8221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     8281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     8341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     8401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     8461 cgcacatttc cccgaaaagt gccacctgac gtc
//