LOCUS pc'_Lyso-AktAR2.xdna 8697 bp DNA circular 4/8/15 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 907..2172 /label=LAMP1 /SerialCloner_Color=&h800040 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="KLAAATMAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIVLIAYLVGRKRSHAGYQTIGGGGG" \ CDS 3451..4185 /label=cpV-E172 /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(6536..6556) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(4250..4267) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(6924..7552) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(6562..6584) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(7704..8363) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 5405..6196 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(8603..8631) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(6589..6618) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 5186..5263 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 4290..4517 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 2182..2898 /label=Cer3 /SerialCloner_Color=&h66CCFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" \ ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAa gcttgcggcc 901 gccaccatgg cggcccccgg cagcgcccgg cgacccctgc tgctgctact gctgttgctg 961 ctgctcggcc tcatgcattg tgcgtcagca gcaatgttta tggtgaaaaa tggcaacggg 1021 accgcgtgca taatggccaa cttctctgct gccttctcag tgaactacga caccaagagt 1081 ggccctaaga acatgacctt tgacctgcca tcagatgcca cagtggtgct caaccgcagc 1141 tcctgtggaa aagagaacac ttctgacccc agtctcgtga ttgcttttgg aagaggacat 1201 acactcactc tcaatttcac gagaaatgca acacgttaca gcgtccagct catgagtttt 1261 gtttataact tgtcagacac acaccttttc cccaatgcga gctccaaaga aatcaagact 1321 gtggaatcta taactgacat cagggcagat atagataaaa aatacagatg tgttagtggc 1381 acccaggtcc acatgaacaa cgtgaccgta acgctccatg atgccaccat ccaggcgtac 1441 ctttccaaca gcagcttcag caggggagag acacgctgtg aacaagacag gccttcccca 1501 accacagcgc cccctgcgcc acccagcccc tcgccctcac ccgtgcccaa gagcccctct 1561 gtggacaagt acaacgtgag cggcaccaac gggacctgcc tgctggccag catggggctg 1621 cagctgaacc tcacctatga gaggaaggac aacacgacgg tgacaaggct tctcaacatc 1681 aaccccaaca agacctcggc cagcgggagc tgcggcgccc acctggtgac tctggagctg 1741 cacagcgagg gcaccaccgt cctgctcttc cagttcggga tgaatgcaag ttctagccgg 1801 tttttcctac aaggaatcca gttgaataca attcttcctg acgccagaga ccctgccttt 1861 aaagctgcca acggctccct gcgagcgctg caggccacag tcggcaattc ctacaagtgc 1921 aacgcggagg agcacgtccg tgtcacgaag gcgttttcag tcaatatatt caaagtgtgg 1981 gtccaggctt tcaaggtgga aggtggccag tttggctctg tggaggagtg tctgctggac 2041 gagaacagca tgctgatccc catcgctgtg ggtggtgccc tggcggggct ggtcctcatc 2101 gtcctcatcg cctacctcgt cggcaggaag aggagtcacg caggctacca gactatcGGT 2161 GGTGGCGGTG GTGCGGATCC Catggtgagc aagggcgagg agctgttcac cggggtggtg 2221 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag 2281 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2341 ctgcccgtgc cctggcccac cctcgtgacc accctgAGCt ggggcgtgca gtgcttcgcc 2401 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2461 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2521 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2581 gacggcaaca tcctggggca caagctggag tacaacgcca tcCACGGCaa cgtctatatc 2641 accgccgaca agcagaagaa cggcatcaag gccAACttcG GCCTCAACTG Caacatcgag 2701 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2761 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2821 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 2881 atggacgagc tgtacaagcg catgcataag ttttctcaag aacagatcgg cgaaaacatt 2941 gtgtgcaggg tcatttgtac cacgggtcaa attcccatcc gagatttgtc agctgatatt 3001 tcacaagtgc ttaaggaaaa acgatccata aagaaagttt ggacatttgg tagaaaccca 3061 gcctgtgact atcatttagg aaacatttca agactgtcaa ataagcattt ccaaatacta 3121 ctaggagaag acggtaacct tttattgaat gacatttcca ctaatgggac ctggttaaat 3181 gggcaaaaag tcgagaagaa cagcaatcag ttactgtctc aaggtgatga aataaccgtt 3241 ggtgtaggcg tggaatcaga tattttatct ctggtcattt tcataaacga caaatttaag 3301 cagtgcctcg agcagaacaa agttgatcgc tctgcaggta agccaggcag cggcgagggc 3361 agcaccaagg gcctggtcga cCCTCGTCCG CGCTCGTGCA CCTGGCCGGA CCCCAGGCCG 3421 GAGTTTGGAG GTACCGGCGG CAGCgagctc atgggcggcg tgcagctcgc cgaccactac 3481 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3541 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3601 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aggtaccggt 3661 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3721 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3781 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg 3841 cccaccctcg tgaccaccct gggctacggc cttcagtgct tcgcccgcta ccccgaccac 3901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 4021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 4081 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag 4141 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgagtaaGA ATTCTGCAGA 4201 TATCCATCAC ACTGGCGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTc tatagtgtca 4261 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 4321 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 4381 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 4441 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 4501 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 4561 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 4621 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 4681 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 4741 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 4801 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 4861 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 4921 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 4981 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 5041 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 5101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 5161 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 5221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 5281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 5341 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 5401 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 5461 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 5521 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 5581 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 5641 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 5701 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 5761 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 5821 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 5881 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 5941 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 6001 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 6061 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 6121 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 6181 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 6241 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 6301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 6361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 6541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 6601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 6661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 6721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 6781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 6841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 6901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 7021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 7081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 7141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 7201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 7261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 7321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 7381 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 7441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 7501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 7561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 7621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 7681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 7741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 7801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 7861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 7921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 8041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 8101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 8161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 8221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 8281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 8341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 8401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 8461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 8521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 8581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 8641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //