LOCUS       pEIGW Grx1-roGFP2        9883 bp ds-DNA     circular     23-MAR-2015
DEFINITION  synthetic circular DNA.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    Untitled 2.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Montag, 23. Mrz 2015 from SnapGene Viewer 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     intron          8942..9007
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     polyA_signal    9582..9716
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3658..3674)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4445..4478
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     source          1..9883
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             2..635
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     LTR             4498..4678
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      6645..7233
                     /direction="RIGHT"
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      7659..7794
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             5614..6474
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     CDS             2885..3601
                     /codon_start=1
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK"
                     /product="roGFP2"
     CDS             9137..9157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             complement(4159..4170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     misc_feature    3688..4276
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    1304..1537
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
     misc_feature    682..807
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2033..2150
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7479..7808
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        2206..2417
                     /note="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        5509..5613
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        complement(4703..4721)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             2786..2878
                     /product="Linker"
     CDS             2465..2782
                     /product="Grx1"
ORIGIN      
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga
     2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
     2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2401 ttgccgccag aacacaggtg tcgtgacgcg gatcgggatc ggcctttacg gccggttacc
     2461 cgggatggct caagagtttg tgaactgcaa aatccagcct gggaaggtgg ttgtgttcat
     2521 caagcccacc tgcccgtact gcaggagggc ccaagagatc ctcagtcaat tgcccatcaa
     2581 acaagggctt ctggaatttg tcgatatcac agccaccaac cacactaacg agattcaaga
     2641 ttatttgcaa cagctcacgg gagcaagaac ggtgcctcga gtctttattg gtaaagattg
     2701 tataggcgga tgcagtgatc tagtctcttt gcaacagagt ggggaactgc tgacgcggct
     2761 aaagcagatt ggagctctgc agactagtgg tggttcaggt ggtggtggtt caggtggtgg
     2821 tggttcaggt ggaggaggat caggaggagg aggatcagga ggaggaggat caggaggaga
     2881 attcgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2941 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3001 cggcaagctg accctgaagt tcatctccac caccggcaag ctgcccgtgc cctggcccac
     3061 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3121 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3181 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3241 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3301 caagctggag tacaactaca actgccacaa cgtctatatc atggccgaca agcagaagaa
     3361 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3421 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3481 ctacctgagc acctgctccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3541 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3601 agcggccgcg actctagcta gtcgagctca acttcgaatt cgatatcaag cttatcgcga
     3661 taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
     3721 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3781 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3841 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3901 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3961 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4021 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4081 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4141 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4201 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4261 tgggccgcct ccccgcatcg ggaattcgag ctcggtcgag gctagtcttg cagagctcgg
     4321 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4381 aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat
     4441 gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg
     4501 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4561 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4621 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     4681 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     4741 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     4801 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     4861 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     4921 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     4981 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     5041 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     5101 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     5161 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     5221 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5281 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5341 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5401 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5461 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5521 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5581 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5641 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5701 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5761 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5821 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5881 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5941 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6001 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6061 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6121 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6181 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6241 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6301 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6361 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6421 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6481 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6541 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6601 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6661 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6721 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6781 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6841 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6901 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6961 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7021 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7081 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7141 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7261 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7321 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7381 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7441 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     7501 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7561 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7621 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7681 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7741 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7801 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     7861 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     7921 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     7981 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     8041 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     8101 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     8161 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     8221 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     8281 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     8341 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     8401 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     8461 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     8521 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     8581 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     8641 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     8701 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     8761 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     8821 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     8881 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     8941 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     9001 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     9061 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     9121 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     9181 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     9241 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     9301 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     9361 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     9421 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     9481 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     9541 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     9601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     9721 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     9781 ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     9841 taaagaaatt gtatttgtta aatatgtact acaaacttag tag
//
