LOCUS pUAST mito roGFP2-Orp1 10449 bp ds-DNA circular 23-MAR-2015 DEFINITION synthetic circular DNA. ACCESSION . (modified) VERSION . KEYWORDS Untitled 6. SOURCE null ORGANISM . REFERENCE 1 (bases 1 to 10449) AUTHORS . TITLE Direct Submission JOURNAL Exported Montag, 23. Mrz 2015 from SnapGene Viewer 2.6.2 http://www.snapgene.com FEATURES Location/Qualifiers intron 1520..1585 /note="small t intron" /note="simian virus 40 (SV40) small t antigen intron" polyA_signal 2160..2294 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter complement(8899..9003) /gene="bla" /note="AmpR promoter" rep_origin complement(7279..7867) /direction="LEFT" /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" sig_peptide 27..125 /product="COX8A" source 1..10449 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 6459..7044 /note="P element 5' end" misc_feature 9805..10037 /note="P element 3' end" CDS join(2799..2870,3265..3538,3613..4267,4329..4644, 4865..4996,5067..5681) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /note="mini-white" /note="This is a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKN YGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNE RHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRL LNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQV IQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTA HSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSY VGAQCPTNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNL EKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFL GQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFL GKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCA SSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWAD VEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLR ARRKE" CDS complement(8038..8898) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" CDS 126..839 /product="roGFP2" CDS 846..935 /product="Linker" CDS 1715..1735 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" CDS 945..1436 /product="Orp1" ORIGIN 1 gaattcgtta acagatctgc ggccgcatgt ccgtcctgac gccgctgctg ctgcggggct 61 tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgccgccgg 121 gatccgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 241 acggcaagct gaccctgaag ttcatctcca ccaccggcaa gctgcccgtg ccctggccca 301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 541 acaagctgga gtacaactac aactgccaca acgtctatat catggccgac aagcagaaga 601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 721 actacctgag cacctgctcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga 841 ctagtggtgg ttcaggtggt ggtggttcag gtggtggtgg ttcaggtgga ggaggatcag 901 gaggaggagg atcaggagga ggaggatcag gaggagaatt cgatatctca gaattctata 961 agctagcacc tgttgacaag aaaggccaac cattcccctt cgaccaatta aagggaaaag 1021 tggtgcttat cgttaatgtt gcctccaaat gtggattcac tcctcaatac aaagaactag 1081 aggccttgta caaacgttat aaggacgaag gatttaccat catcgggttc ccatgcaacc 1141 agtttggcca ccaagaacct ggctctgatg aagaaattgc ccagttctgc caactgaact 1201 atggcgtgac tttccccatt atgaaaaaaa ttgacgttaa tggtggcaat gaggaccctg 1261 tttacaagtt tttgaagagc caaaaatccg gtatgttggg cttgagaggt atcaaatgga 1321 attttgaaaa attcttagtc gataaaaagg gtaaagtgta cgaaagatac tcttcactaa 1381 ccaaaccttc ttcgttgtcc gaaaccatcg aagaactttt gaaagaggtg gaatagtcta 1441 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac 1501 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact 1561 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg 1621 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga 1681 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga 1741 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag 1801 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa 1861 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat 1921 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa 1981 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag 2041 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa 2101 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 2161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 2221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 2281 atcatgtctg gatcggatcc actagaaggc cttagtatgt atgtaagtta ataaaaccct 2341 tttttggaga atgtagattt aaaaaaacat attttttttt tattttttac tgcactggac 2401 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgaagtacc 2461 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc 2521 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt 2581 cgccaccgtt tgtagcgtta cctagcgtca atgtccgcct tcagttgcac tttgtcagcg 2641 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa 2701 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt 2761 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc 2821 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta 2881 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg 2941 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaatcgcac aaacaaatag 3001 tgacacgaaa cagattattc tggtagctgt gctcgctata taagacaatt tttaagatca 3061 tatcatgatc aagacatcta aaggcattca ttttcgacta cattcttttt tacaaaaaat 3121 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac 3181 ctacttcgta ggatacttcg ttttgttcgg ggttagatga gcataacgct tgtagttgat 3241 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac 3301 cagggcttcg ggcaggccaa aaactacggc acgctcctgc cacccagtcc gccggaggac 3361 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac 3421 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc 3481 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt 3541 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa 3601 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt 3661 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc 3721 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc 3781 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg 3841 aacacctgat tttccaggcc atggtgcgga tgccacgaca tctgacctat cggcagcgag 3901 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca 3961 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg 4021 cctccgaggc actaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg 4081 actcatttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga 4141 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc 4201 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct 4261 tttcctagtg agttcgatgt gtttattaag ggtatctagc attacattac atctcaactc 4321 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag 4381 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc 4441 gacaattttg ctattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat 4501 ttggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg 4561 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta 4621 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct 4681 tacaattctt ggaaacaaat tcgctagatt ttagttagaa ttgcctgatt ccacaccctt 4741 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaaaataaa taaatttcat 4801 ttaactcgcg aacatgttga agatatgaat attaatgaga tgcgagtaac attttaattt 4861 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg 4921 cgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt 4981 ctttgccacg ataaatgtaa gtcttgttta gaatacattt gcatattaat aatttactaa 5041 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg 5101 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat 5161 taccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac 5221 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg 5281 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt 5341 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg 5401 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg 5461 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt 5521 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg 5581 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg 5641 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata 5701 actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa 5761 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt 5821 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt 5881 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata 5941 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc 6001 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt 6061 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt 6121 ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat 6181 ttgctccaat atatttgtat atatttccct cacagctata tttattctaa tttaatatta 6241 tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact 6301 gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata 6361 atagtgttgt ttatatatat caaaaataac aactataata ataagaatac atttaattta 6421 gaaaatgctt ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat 6481 ttcgtactcc aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca 6541 ggacctaacg cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt 6601 actctgtttg tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta 6661 tcactttact aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg 6721 caggtgcagc cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac 6781 caaggctgca cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt 6841 gaattaattt tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt 6901 attccacgta agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc 6961 aacaagcaaa cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg 7021 accaccttat gttatttcat catgggccag acccacgtag tccagcggca gatcggcggc 7081 ggagaagtta agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga 7141 tgtgcagcta atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt 7201 ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga 7261 gttcaagaag aaggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7441 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 7501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8341 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8641 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9001 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9061 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9121 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9181 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9241 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9301 gcaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg 9361 tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg tgtggtggag 9421 gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata tatcccggtg 9481 ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt 9541 tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac ttgtattcta 9601 taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt gagggggcaa 9661 taaacagtaa acacgatggt aataatggta aaaaaaaaaa caagcagtta tttcggatat 9721 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga 9781 agctcacgat gagaatggcc agaccatgat gaaataacat aaggtggtcc cgtcggcaag 9841 agacatccac ttaacgtatg cttgcaataa gtgcgagtga aaggaatagt attctgagtg 9901 tcgtattgag tctgagtgag acagcgatat gattgttgat taacccttag catgtccgtg 9961 gggtttgaat taactcataa tattaattag acgaaattat ttttaaagtt ttatttttaa 10021 taatttgcga gtacgcaaag cttgggctgc atctctccgg atccaagctt gcatgcctgc 10081 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga 10141 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg 10201 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga 10261 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc 10321 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa 10381 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata 10441 gggaattgg //