LOCUS Exported 5011 bp ds-DNA circular SYN 17-APR-2015 DEFINITION pETDUET-1. ACCESSION . VERSION . KEYWORDS pCRISPReporter-mCherry for addgene SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5011) AUTHORS Bob Novy TITLE Direct Submission JOURNAL Exported Friday, Apr 17, 2015 from SnapGene Viewer 2.6.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5011 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 39..128 /note="ECK120029600 Terminator (Voight)" /note="rho-independent terminator of spheroplast protein Y transcript" misc_feature 149..204 /note="MCS" CDS 205..249 /note="(GGGGS)3 flexible linker" CDS 256..963 /note="mCherry" terminator 970..1026 /note="ECK120033737 Terminator (Voight)" /note="rho-independent terminator of thrLABC operon" rep_origin 1108..1555 /note="f1 ori" CDS complement(1677..2537) /note="Ap" /note="B-lactamase gene" -10_signal complement(2579..2585) /note="-10 Ap" -35_signal complement(2603..2608) /note="-35 Ap" rep_origin 3298 /note="ColE1, pBR322 ori" CDS complement(3729..3920) /note="ROP" ORIGIN 1 gggcccgctg ggagttcgta gacggaccta ggtctagatt cagccaaaaa acttaagacc 61 gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt tttcttttct 121 cttctcaaca ttactcgcat ccattctcgg cgatcgcgcg gccgctttaa ttaagaattc 181 aagcttagat ctcaattgct cgagggcggt ggcggtagtg gcggtggcgg ttccggcggt 241 ggcggttcac atatggtttc aaaaggcgaa gaagacaaca tggcgattat caaggaattt 301 atgcgtttca aggtccacat ggaaggcagc gtcaatggtc acgaatttga aattgaaggc 361 gaaggtgaag gccgtccgta tgaaggcacc cagacggcaa aactgaaggt caccaaaggc 421 ggtccgctgc cgtttgcttg ggatattctg tcaccgcaat tcatgtatgg ttcgaaagcg 481 tacgttaagc atccggccga tatcccggac tatctgaaac tgtcctttcc ggaaggcttc 541 aaatgggaac gtgttatgaa cttcgaagat ggcggtgtgg ttaccgtcac gcaggatagc 601 tctctgcaag acggtgaatt tatttataaa gtgaagctgc gcggcaccaa tttcccgagc 661 gatggtccgg ttatgcagaa aaagacgatg ggctgggaag cgagttccga acgtatgtac 721 ccggaagacg gtgccctgaa aggcgaaatc aagcagcgcc tgaaactgaa ggatggcggt 781 cactatgacg cagaagtgaa aaccacgtac aaggctaaaa agccggtcca actgccgggt 841 gcatacaacg tgaacatcaa gctggatatc accagccata acgaagacta tacgatcgtt 901 gaacagtacg aacgtgcaga aggccgccac tctaccggcg gtatggatga actgtacaaa 961 taaggtaccg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac 1021 caaaggaggc tgtctcgtct cgtctcacta gtgctagcaa caaacgcaga atccaagcgt 1081 cgactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 1141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 1201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 1261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 1321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 1381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 1441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 1501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 1561 ttctggcggc acgatggcat gagattatca aaaaggatct tcacctagat ccttttaaat 1621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 1681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 1741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 1801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 1861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 1921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 1981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 2041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 2101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 2161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 2221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 2281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 2341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 2401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 2461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 2521 aaatgttgaa tactcatact cttccttttt caatcatgat tgaagcattt atcagggtta 2581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggtcatga 2641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 2701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2821 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 3001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 3061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 3121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 3421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 3481 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg 3541 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta 3601 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc 3661 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 3721 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc 3781 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg 3841 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg 3901 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta 3961 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc 4021 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga 4081 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt 4141 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc 4201 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca 4261 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa 4321 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcta 4381 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt 4441 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc 4501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 4561 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa 4621 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt 4681 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct 4741 gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc 4801 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg 4861 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc 4921 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc 4981 cagacgcaga cgcgccgaga cagaacttaa t //