LOCUS       AY597269                2958 bp    DNA     circular SYN 25-MAY-2005
DEFINITION  FRT source vector pFRT2, complete sequence.
ACCESSION   AY597269
VERSION     AY597269.1  GI:47027943
KEYWORDS    .
SOURCE      FRT source vector pFRT2
  ORGANISM  FRT source vector pFRT2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Choi,K.H., Gaynor,J.B., White,K.G., Lopez,C., Bosio,C.M.,
            Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
   PUBMED   15908923
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Choi,K.H., Gaynor,J., Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     Improved FRT cassettes and Flp expression vectors
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2958)
  AUTHORS   Choi,K.H., Gaynor,J., Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2004) Microbiology, Immunology and Pathology,
            Colorado State University, Fort Collins, CO 80523, USA
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="FRT source vector pFRT2"
                     /mol_type="other DNA"
                     /db_xref="taxon:274579"
     misc_feature    399..449
                     /note="polylinker"
     misc_feature    464..511
                     /note="FRT; Flp recombinase target site"
     misc_feature    565..570
                     /note="EcoRV cloning site"
     misc_feature    604..651
                     /note="FRT; Flp recombinase target site"
     misc_feature    674..724
                     /note="polylinker"
     gene            complement(1898..2758)
                     /gene="bla"
     CDS             complement(1898..2758)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAT08986.1"
                     /db_xref="GI:47027944"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattggggat cttgaagtac ctattccgaa
      481 gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaagctga tgtgcttaaa
      541 aacttactca atggaataat tctagatatc cattgctgtt gacaaaggga atcaggggat
      601 cttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt
      661 ttgaagctaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
      721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      901 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      961 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1021 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1561 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1741 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1801 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1861 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1921 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1981 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2041 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2101 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2161 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2221 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2281 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2341 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2401 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2461 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2521 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2581 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2641 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2701 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2761 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2821 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2881 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2941 tcacgaggcc ctttcgtc
//

