LOCUS       DQ296496                7430 bp    DNA     circular SYN 07-DEC-2005
DEFINITION  Mini-Tn7 delivery vector pUC18-mini-Tn7T-Gm-lacZ30, complete
            sequence.
ACCESSION   DQ296496
VERSION     DQ296496.1  GI:83033226
KEYWORDS    .
SOURCE      Mini-Tn7 delivery vector pUC18-mini-Tn7T-Gm-lacZ30
  ORGANISM  Mini-Tn7 delivery vector pUC18-mini-Tn7T-Gm-lacZ30
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Choi,K.H., Gaynor,J.B., White,K.G., Lopez,C., Bosio,C.M.,
            Karkhoff-Schweizer,R.R. and Schweizer,H.P.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
   PUBMED   15908923
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Choi,K.-H. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2005) Microbiology, Immunology and Pathology,
            Colorado State University, Campus Delivery 1682, Fort Collins, CO
            80523, USA
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Mini-Tn7 delivery vector
                     pUC18-mini-Tn7T-Gm-lacZ30"
                     /mol_type="other DNA"
                     /db_xref="taxon:360167"
     repeat_region   382..580
                     /note="Tn7R; right end of Tn7"
                     /rpt_type=inverted
     regulatory      629..723
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
     regulatory      820..917
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
     misc_recomb     958..1005
                     /note="FRT; Flp recombinase target site"
     gene            complement(1135..1668)
                     /gene="aacC1"
     CDS             complement(1135..1668)
                     /gene="aacC1"
                     /codon_start=1
                     /transl_table=11
                     /product="gentamycin acetyl transferase"
                     /protein_id="ABB97076.1"
                     /db_xref="GI:83033227"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
     misc_recomb     1926..1973
                     /note="FRT; Flp recombinase target site"
     misc_feature    2000..2047
                     /note="MCS; multiple cloning site"
     gene            <2052..5102
                     /gene="lacZ"
     CDS             <2052..5102
                     /gene="lacZ"
                     /note="designed for transcriptional fusion as a reporter
                     gene"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="ABB97077.1"
                     /db_xref="GI:83033228"
                     /translation="PVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQL
                     RSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITV
                     NPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDF
                     HVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERG
                     GYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGL
                     LLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAV
                     PKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQ
                     SLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRL
                     SGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPE
                     SAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDF
                     CIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNA
                     WVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQ
                     MAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLP
                     LSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAE
                     EGTWLNIDGFHMGIGGDDSWSPSVSADFQLSAGRYHYQLVWCQK"
     repeat_region   5176..5341
                     /note="Tn7L; left end of Tn7"
                     /rpt_type=inverted
     gene            complement(6370..7230)
                     /gene="bla"
     CDS             complement(6370..7230)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABB97078.1"
                     /db_xref="GI:83033229"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cgaattcccg gggatccgtc gacctgcagc
     2041 caagcttcga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2101 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2161 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
     2221 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     2281 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
     2341 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     2401 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     2461 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     2521 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
     2581 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc
     2641 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
     2701 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
     2761 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
     2821 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
     2881 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
     2941 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     3001 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     3061 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
     3121 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
     3181 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
     3241 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     3301 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
     3361 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
     3421 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
     3481 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
     3541 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     3601 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
     3661 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
     3721 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
     3781 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
     3841 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
     3901 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
     3961 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
     4021 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
     4081 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
     4141 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
     4201 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
     4261 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
     4321 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     4381 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     4441 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
     4501 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
     4561 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
     4621 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     4681 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     4741 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     4801 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     4861 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     4921 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     4981 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     5041 cggcggattt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     5101 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
     5161 atttccttgc aggccaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     5221 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     5281 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     5341 agcggggcat ttttcttcct gttatgtttg ggcgctgcat taatgaatcg gccaacgcgc
     5401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     7381 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//

