LOCUS       DQ493881                5506 bp    DNA     circular SYN 25-MAY-2010
DEFINITION  Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Tp-gfpmut3, complete
            sequence.
ACCESSION   DQ493881
VERSION     DQ493881.2  GI:296840618
KEYWORDS    .
SOURCE      Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Tp-gfpmut3
  ORGANISM  Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Tp-gfpmut3
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Choi,K.-H. and Schweizer,H.P.
  TITLE     Mini-Tn7 insertion in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Choi,K.-H. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2006) Microbiology, Immunology and Pathology,
            Colorado State University, 1682 Campus Delivery, Fort Collins, CO
            80523-1682, USA
REFERENCE   3  (bases 1 to 5506)
  AUTHORS   Choi,K.-H. and Schweizer,H.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Microbiology, Immunology and Pathology,
            Colorado State University, 1682 Campus delivery, Ft. Collins, CO
            80523, USA
  REMARK    Sequence update by submitter
COMMENT     On May 25, 2010 this sequence version replaced gi:104716981.
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Mini-Tn7 delivery vector
                     pUC18T-mini-Tn7T-Tp-gfpmut3"
                     /mol_type="other DNA"
                     /db_xref="taxon:383990"
     mobile_element  382..580
                     /note="right end of Tn7"
                     /mobile_element_type="transposon:Tn7R"
     regulatory      629..723
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
     regulatory      820..917
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
     misc_feature    963..1010
                     /note="FRT; Flp recombinase target site"
     gene            complement(1072..1308)
                     /gene="dhfRII"
     CDS             complement(1072..1308)
                     /gene="dhfRII"
                     /note="trimethoprim resistance gene"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim resistance protein"
                     /protein_id="ABF74539.1"
                     /db_xref="GI:104716982"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
                     WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    1713..1760
                     /note="FRT; Flp recombinase target site"
     regulatory      complement(1887..1984)
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
     regulatory      complement(2081..2175)
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
     gene            complement(2195..2911)
                     /gene="gfpmut3"
     CDS             complement(2195..2911)
                     /gene="gfpmut3"
                     /note="Gfpmut3"
                     /codon_start=1
                     /transl_table=11
                     /product="green fluorescent protein mutant 3"
                     /protein_id="ABF74540.1"
                     /db_xref="GI:104716983"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
     regulatory      complement(2940..3010)
                     /regulatory_class="promoter"
                     /note="rrnB P1"
     mobile_element  3023..3188
                     /note="left end of Tn7"
                     /mobile_element_type="transposon:Tn7L"
     misc_feature    3340..3608
                     /note="oriT; origin of transfer"
     gene            complement(4446..5306)
                     /gene="bla"
     CDS             complement(4446..5306)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABF74541.1"
                     /db_xref="GI:104716984"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atgagctcga attggggatc
      961 ttgaagtacc tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt
     1021 tgaagctgat gtgcttaaaa acttactcaa tggaataatt ctagataatt cttaggccac
     1081 acgttcaagt gcagccacag gataaatttg cactgagcct gggtgggatt cggactcgac
     1141 cgcatagcct tcaggagtga gttttgtgca ataccaaccg acgacttgac cctgccaagc
     1201 ggcaccagat ttcttgcgta cgcgatcccc taagccaaag gtggcactca ggggaagcgc
     1261 aaactgccct gcaacgggag cgttggcttc atcgctactt tgacccatgt cgaatccttc
     1321 ttgtgaatct attatggcga caagcaaatc gagctctgac tgcctacccc acaacaacta
     1381 tcagaaagca ccagcacaac ggctgcctaa ctttgtttta gggcgactgc cctgctgcgt
     1441 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     1501 ggatgcccga ggctagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     1561 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     1621 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgaattatcc
     1681 attgctgttg acaaagggaa tcaggggatc ttgaagttcc tattccgaag ttcctattct
     1741 ctagaaagta taggaacttc agagcgcttt tgaagctaat tcgagctcga tcatgcatga
     1801 gctcactagt ggatcccccg ggctgcagga attcctcgag aagcttgggc ccggtacctc
     1861 gcgaaggcgg gccgcaagct cctagcggcg gatttgtcct actcaggaga gcgttcaccg
     1921 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     1981 gcctcaagct agagagtcat taccccaggc gtttaagggc accaataact gccttaaaaa
     2041 aattacgccc cgccctgcca ctcatcgcag tctagcttgg attctcacca ataaaaaacg
     2101 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     2161 ctatcaacag gagtccaagc tcagctaatt aagcttattt gtatagttca tccatgccat
     2221 gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg
     2281 gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc
     2341 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa
     2401 tgttgtgtct aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt
     2461 atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt
     2521 taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca aacttgactt
     2581 cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac
     2641 cttcgggcat ggcactcttg aaaaagtcat gccgtttcat atgatctggg tatctcgcaa
     2701 agcattgaac accataaccg aaagtagtga caagtgttgg ccatggaaca ggtagttttc
     2761 cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc
     2821 cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa
     2881 ctccagtgaa aagttcttct cctttacgca tgcttaattt ctcctcttta attctagagt
     2941 ggtgtcgcat tatagggagt tattccggcc tgactagagg aaatttaaaa taattttctg
     3001 accgcgcaac gccttgcagg ccaaccagat aagtgaaatc tagttccaaa ctattttgtc
     3061 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     3121 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     3181 cgcccacagc ggggcatttt tcttcctgtt atgtttgggc gctgcattaa tgaatcggcc
     3241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgacc
     3301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcaga gcttatcggc cagcctcgca
     3361 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     3421 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     3481 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     3541 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaagt ataccttaag
     3601 gaatcccctg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3721 aaaaatcgac gctcgagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5461 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//

