LOCUS       FLIP2_delCFPfiller        5431 bp ds-DNA   circular     31-AUG-2010
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    19..37
                     /note="T7 primer"
                     /label=T7 primer
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    111..128
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    complement(3512..3529)
                     /note="T7 rev"
                     /label=T7 rev
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    3669..3731
                     /note="f1 ori repli"
                     /label=f1 ori repli
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    4222..4308
                     /note="Bla promoter"
                     /label=Bla promoter
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    4310..4930
                     /note="Ampicillin"
                     /label=Ampicillin
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    202..206
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    3423..3452
                     /note="cMyc tag"
     misc_feature    921..926
                     /note="KpnI"
     misc_feature    2709..3422
                     /note="Cerulean"
     misc_feature    207..920
                     /note="Citrine"
     misc_feature    933..1057
                     /note="attR1"
                     /label=attR1
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     gene            1166..1757
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    1759..1825
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2225..2531
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2571..2695
                     /note="attR2"
                     /label=attR2
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
ORIGIN      
          1 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA
         61 GAaaTAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC
        121 ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG
        181 ATGACGATAA GGATCCGGGC CTCGAGgtga gcaagggcga ggagctgttc accggggtgg
        241 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
        301 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
        361 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
        421 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
        481 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
        541 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
        601 aggacggcaa catcctgggg cacaagcttg agtacaacta caacagccac aacgtctata
        661 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
        721 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
        781 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgAGC aaagacccca
        841 acgagaagcg cgatcacatg gtcctgctgg agttcGTGac cgccgccggg atcactctcg
        901 gcatggacga gctgtacaag GGTACCGATA TCACAAGTTT GTACAAAAAA GCTGAACGAG
        961 AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA
       1021 CATAATACTG TAAAACACAA CATATCCAGT CAcTATGGCG GCCGCATTAG GCACCCCAGG
       1081 CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG
       1141 ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA
       1201 TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC
       1261 CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA
       1321 GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA
       1381 ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA
       1441 CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA
       1501 TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC
       1561 CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG
       1621 TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC
       1681 CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA
       1741 TCATGCCGTT TGTGATGgGC TTCCATGTCG GCAGAATGCT TAATGAATTA CACAGTACTG
       1801 CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG CCAGATAACA
       1861 GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG
       1921 AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC
       1981 AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA
       2041 TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA
       2101 TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT
       2161 GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAACTTTTG CTGACGAGAA CAGGGGCTGG
       2221 TGAAATGCAG TTTAAGGTTT ACACCTATAA AAGAGAGAGC CGTTATCGTC TGTTTGTGGA
       2281 TGTACAGAGT GATATTATTG ACACGCCCGG GCGACGGATG GTGATCCCCC TGGCCAGTGC
       2341 ACGTCTGCTG TCAGATAAAG TCTCCCGTGA ACTTTACCCG GTGGTGCATA TCGGGGATGA
       2401 AAGCTGGCGC ATGATGACCA CCGATATGGC CAGTGTGCCG GTCTCCGTTA TCGGGGAAGA
       2461 AGTGGCTGAT CTCAGCCACC GCGAAAATGA CATCAAAAAC GCCATTAACC TGATGTTCTG
       2521 GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT GCAGGTCGAC CATAGTGACT
       2581 GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA
       2641 TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT CTTGTACAAA GTGGTGATAT
       2701 CGACTAGTgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
       2761 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
       2821 cctacggcaa gctgaccctg aagttcatct gcaccaccgg taagctgccc gtgccctggc
       2881 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
       2941 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
       3001 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
       3061 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
       3121 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
       3181 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
       3241 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
       3301 accactacct gagcacccag tccGCCctgA GCaaagaccc caacgagaag cgcgatcaca
       3361 tggtcctgct ggagttcGTG accgccgccg ggatcactct cggcatggac gagctgtaca
       3421 agGAACAAAA ATTGATAAGT GAGGAAGATT TATAAGGGCC CGATCCGGCT GCTAACAAAG
       3481 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
       3541 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATCTG
       3601 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
       3661 CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
       3721 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT
       3781 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT
       3841 CCGATTTAGA GCTTTACGGC ACCTCGACCG CAAAAAACTT GATTTGGGTG ATGGTTCACG
       3901 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT
       3961 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCGCGG TCTATTCTTT
       4021 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA
       4081 AATATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCGCCTG ATGCGGTATT
       4141 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACA GGTGGCACTT TTCGGGGAAA
       4201 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
       4261 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
       4321 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
       4381 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
       4441 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
       4501 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGATACA CTATTATCCC GTATTGACGC
       4561 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
       4621 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
       4681 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
       4741 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
       4801 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGAGTGAC ACCACGATGC CTGTAGCAAT
       4861 GCCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
       4921 ATTAATAGAC TGAATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
       4981 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT
       5041 TGCAGCACTG GGGCCAGATG GTAAGCGCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
       5101 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
       5161 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
       5221 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
       5281 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
       5341 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       5401 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
       5461 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
       5521 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
       5581 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
       5641 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
       5701 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
       5761 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
       5821 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
       5881 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
       5941 CGCGGCCTTT TTACGGTTCC TGGGCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
       6001 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
       6061 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
       6121 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGg
//

