LOCUS       pDRFLIP32               9724 bp    DNA     circular     01-MAY-2015
Created: Mittwoch, 30. Mai 2001 11:16
FEATURES             Location/Qualifiers
     exon            complement(6130..6933)
                     /note="URA3"
     terminator      3881..4213
                     /note="tADH"
     rep_origin      complement(4673..5837)
                     /note="2%B5"
     gene            7363..8225
                     /note="AmpR"
     rep_origin      complement(8393..9046)
                     /note="pUC ORI"
     misc_feature    507..524
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     promoter        1..452
                     /note="pPMA1"
     misc_feature    598..602
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    603..1289
                     /note="AFPt9"
     gene            3078..3746
                     /note="t7CFPt9"
     misc_feature    3747..3776
                     /note="cMyc tag"
     misc_feature    1302..1426
                     /note="attR1"
     gene            1535..2126
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2128..2194
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2594..2900
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2940..3064
                     /note="attR2"
ORIGIN      
          1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
         61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
        121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
        181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
        241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
        301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
        361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
        421 aaagaaagaa aaaaaatata ccccagcctc gaTctagAaa TAATTTTGTT TAACTTTAAG
        481 AAGGAGATAT ACATATGCGG GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA
        541 CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT CCGGGCCTCG
        601 AGGTTTCTAA AGGTGAAGAA TTGTTTACGG GCGTCGTCCC GATCCTCGTG GAACTCGACG
        661 GGGATGTTAA CGGGCATAAG TTTTCGGTCA GCGGGGAAGG GGAGGGGGAC GCGACGTATG
        721 GGAAGCTCAC TCTCAAGCTG ATCTGTACGA CGGGGAAACT CCCGGTCCCG TGGCCGACGC
        781 TGGTCACGAC GCTGGGATAC GGGCTCCAAT GCTTTGCGAG GTATCCGGAC CACATGAAAC
        841 AGCATGACTT TTTCAAATCG GCGATGCCGG AGGGATACGT GCAGGAACGG ACGATCTTTT
        901 TCAAAGACGA TGGGAACTAT AAGACGCGGG CGGAAGTCAA GTTTGAAGGG GACACGCTCG
        961 TCAACCGGAT CGAACTCAAG GGGATTGACT TCAAAGAGGA TGGGAACATA CTCGGCCATA
       1021 AGCTCGAATA CAATTACAAC TCGCATAACG TATACATCAC CGCGGATAAG CAAAAGAACG
       1081 GGATCAAAGC CAATTTCAAA ATCCGGCATA ACATAGAGGA TGGGGGGGTC CAACTGGCGG
       1141 ATCACTATCA GCAAAACACG CCGATAGGGG ATGGGCCGGT CCTCCTCCCG GATAACCATT
       1201 ACCTCTCGTA CCAAAGCGCG CTCTCGAAGG ACCCGAATGA GAAACGGGAC CACATGGTTC
       1261 TCCTGGAGTT CGTCACGGCG GCGGGCATAG GTACCGATAT CACAAGTTTG TACAAAAAAG
       1321 CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA
       1381 AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC AcTATGGCGG CCGCATTAGG
       1441 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA
       1501 TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC
       1561 CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC
       1621 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA
       1681 GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC
       1741 TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA
       1801 CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA
       1861 CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA
       1921 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC
       1981 CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC
       2041 CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT
       2101 TCAGGTTCAT CATGCCGTTT GTGATGgGCT TCCATGTCGG CAGAATGCTT AATGAATTAC
       2161 ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT GGATCCGGCT TACTAAAAGC
       2221 CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT ATACTGATAT
       2281 GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC AGCGTATTAC AGTGACAGTT
       2341 GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC GGTCTGGTAA
       2401 GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA GCGGAAAATC
       2461 AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT GCTGACGAGA
       2521 ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAACTTTTGC TGACGAGAAC
       2581 AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT
       2641 GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT
       2701 GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT
       2761 CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT
       2821 CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT
       2881 GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC
       2941 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
       3001 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG
       3061 TGGTGATATC GACTAGTctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
       3121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
       3181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
       3241 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
       3301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
       3361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
       3421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       3481 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
       3541 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
       3601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
       3661 tgagcaccca gtccgccctg AGCaaagacc ccaacgagaa gcgcgatcac atggtcctgc
       3721 tggagttcGT Gaccgccgcc gggatcGAAC AAAAATTGAT AAGTGAGGAA GATTTATAAG
       3781 GGccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
       3841 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg gatccagctt tggacttctt
       3901 cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat
       3961 ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca cttctaaata
       4021 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
       4081 atacaaattt taaagtgact cttaggtttt aaaacgaraa ttcttattct tgagtaactc
       4141 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
       4201 ctctaccggc atgccaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       4261 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
       4321 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
       4381 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat aatcggatcg
       4441 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
       4501 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
       4561 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
       4621 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
       4681 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
       4741 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
       4801 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
       4861 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
       4921 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
       4981 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
       5041 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
       5101 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
       5161 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
       5221 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
       5281 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
       5341 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
       5401 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
       5461 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
       5521 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
       5581 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
       5641 tcctatactt tctagctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
       5701 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
       5761 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
       5821 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
       5881 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
       5941 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
       6001 tttcctttga tattggatcg atccgatgat aagctgtcaa acatgagaat tgggtaataa
       6061 ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa
       6121 tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct
       6181 gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat
       6241 aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc
       6301 gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc
       6361 tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc
       6421 aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc
       6481 tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc
       6541 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
       6601 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
       6661 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
       6721 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
       6781 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
       6841 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
       6901 agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc tgcatgtttt
       6961 tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat
       7021 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat
       7081 taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga
       7141 tgaattgaaa agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
       7201 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
       7261 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
       7321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
       7381 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
       7441 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
       7501 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
       7561 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
       7621 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
       7681 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
       7741 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
       7801 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
       7861 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
       7921 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
       7981 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
       8041 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
       8101 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
       8161 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
       8221 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
       8281 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
       8341 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       8401 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
       8461 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
       8521 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
       8581 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
       8641 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
       8701 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
       8761 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
       8821 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
       8881 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
       8941 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
       9001 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
       9061 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
       9121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
       9181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       9241 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
       9301 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
       9361 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttcctgaa acggagaaac
       9421 ataaacaggc attgctggga tcacccatac atcactctgt tttgcctgac cttttccggt
       9481 aatttgaaaa caaacccggt ctcgaagcgg agatccggcg ataattaccg cagaaataaa
       9541 cccatacacg agacgtagaa ccagccgcac atggccggag aaactcctgc gagaatttcg
       9601 taaactcgcg cgcattgcat ctgtatttcc taatgcggca cttccaggcc tcgatcgaga
       9661 ccgtttatcc attgcttttt tgttgtcttt ttccctcgtt cacagaaagt ctgaagaagc
       9721 tata
//

