LOCUS       pDRFLIP34               9733 bp    DNA     circular     01-MAY-2015
Created: Mittwoch, 30. Mai 2001 11:16
FEATURES             Location/Qualifiers
     exon            complement(6139..6942)
                     /note="URA3"
     terminator      3890..4222
                     /note="tADH"
     rep_origin      complement(4682..5846)
                     /note="2%B5"
     gene            7372..8234
                     /note="AmpR"
     rep_origin      complement(8402..9055)
                     /note="pUC ORI"
     misc_feature    507..524
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     promoter        1..452
                     /note="pPMA1"
     misc_feature    598..602
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    603..1289
                     /note="AFPt9"
     gene            3078..3755
                     /note="TFPt9"
     misc_feature    3756..3785
                     /note="cMyc tag"
     misc_feature    1302..1426
                     /note="attR1"
     gene            1535..2126
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2128..2194
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2594..2900
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2940..3064
                     /note="attR2"
ORIGIN      
          1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
         61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
        121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
        181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
        241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
        301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
        361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
        421 aaagaaagaa aaaaaatata ccccagcctc gaTctagAaa TAATTTTGTT TAACTTTAAG
        481 AAGGAGATAT ACATATGCGG GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA
        541 CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT CCGGGCCTCG
        601 AGGTTTCTAA AGGTGAAGAA TTGTTTACGG GCGTCGTCCC GATCCTCGTG GAACTCGACG
        661 GGGATGTTAA CGGGCATAAG TTTTCGGTCA GCGGGGAAGG GGAGGGGGAC GCGACGTATG
        721 GGAAGCTCAC TCTCAAGCTG ATCTGTACGA CGGGGAAACT CCCGGTCCCG TGGCCGACGC
        781 TGGTCACGAC GCTGGGATAC GGGCTCCAAT GCTTTGCGAG GTATCCGGAC CACATGAAAC
        841 AGCATGACTT TTTCAAATCG GCGATGCCGG AGGGATACGT GCAGGAACGG ACGATCTTTT
        901 TCAAAGACGA TGGGAACTAT AAGACGCGGG CGGAAGTCAA GTTTGAAGGG GACACGCTCG
        961 TCAACCGGAT CGAACTCAAG GGGATTGACT TCAAAGAGGA TGGGAACATA CTCGGCCATA
       1021 AGCTCGAATA CAATTACAAC TCGCATAACG TATACATCAC CGCGGATAAG CAAAAGAACG
       1081 GGATCAAAGC CAATTTCAAA ATCCGGCATA ACATAGAGGA TGGGGGGGTC CAACTGGCGG
       1141 ATCACTATCA GCAAAACACG CCGATAGGGG ATGGGCCGGT CCTCCTCCCG GATAACCATT
       1201 ACCTCTCGTA CCAAAGCGCG CTCTCGAAGG ACCCGAATGA GAAACGGGAC CACATGGTTC
       1261 TCCTGGAGTT CGTCACGGCG GCGGGCATAG GTACCGATAT CACAAGTTTG TACAAAAAAG
       1321 CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA
       1381 AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC AcTATGGCGG CCGCATTAGG
       1441 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA
       1501 TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC
       1561 CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC
       1621 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA
       1681 GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC
       1741 TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA
       1801 CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA
       1861 CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA
       1921 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC
       1981 CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC
       2041 CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT
       2101 TCAGGTTCAT CATGCCGTTT GTGATGgGCT TCCATGTCGG CAGAATGCTT AATGAATTAC
       2161 ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT GGATCCGGCT TACTAAAAGC
       2221 CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT ATACTGATAT
       2281 GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC AGCGTATTAC AGTGACAGTT
       2341 GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC GGTCTGGTAA
       2401 GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA GCGGAAAATC
       2461 AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT GCTGACGAGA
       2521 ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAACTTTTGC TGACGAGAAC
       2581 AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT
       2641 GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT
       2701 GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT
       2761 CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT
       2821 CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT
       2881 GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC
       2941 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
       3001 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG
       3061 TGGTGATATC GACTAGTgtg agcaagggcg aggagaccac aatgggcgta atcaagcccg
       3121 acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc gtgatcgagg
       3181 gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag gtgaaggagg
       3241 gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac ggcaacaggg
       3301 ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc cccgagggct
       3361 actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg aagtccgaca
       3421 tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag aacttccccc
       3481 ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc gagaggatgt
       3541 acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg gagggcggcg
       3601 gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg aagctgcccg
       3661 actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac tacaacaagg
       3721 tgaccgttta cgagagcgcc gtggcccgca actccGAACA AAAATTGATA AGTGAGGAAG
       3781 ATTTATAAGG Gccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
       3841 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt
       3901 ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt
       3961 gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac
       4021 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
       4081 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaraat tcttattctt
       4141 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
       4201 tgaccacacc tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg
       4261 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
       4321 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
       4381 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
       4441 atcggatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
       4501 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
       4561 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
       4621 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
       4681 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
       4741 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
       4801 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
       4861 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
       4921 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
       4981 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
       5041 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
       5101 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
       5161 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
       5221 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
       5281 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
       5341 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
       5401 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
       5461 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
       5521 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
       5581 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
       5641 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
       5701 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
       5761 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
       5821 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
       5881 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
       5941 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
       6001 atgctatcat ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt
       6061 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
       6121 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
       6181 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
       6241 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
       6301 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
       6361 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
       6421 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
       6481 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
       6541 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
       6601 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
       6661 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
       6721 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
       6781 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
       6841 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
       6901 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
       6961 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
       7021 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
       7081 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
       7141 aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
       7201 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
       7261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
       7321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       7381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
       7441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
       7501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
       7561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
       7621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
       7681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
       7741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
       7801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
       7861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
       7921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
       7981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
       8041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
       8101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
       8161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
       8221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
       8281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       8341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
       8401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
       8461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
       8521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
       8581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
       8641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
       8701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
       8761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       8821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
       8881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
       8941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       9001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
       9061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
       9121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
       9181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       9241 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
       9301 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
       9361 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttcctgaaa
       9421 cggagaaaca taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc
       9481 ttttccggta atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc
       9541 agaaataaac ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg
       9601 agaatttcgt aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct
       9661 cgatcgagac cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc
       9721 tgaagaagct ata
//

