LOCUS       pDRFLIP35               9733 bp    DNA     circular     21-SEP-2015
Created: Mittwoch, 30. Mai 2001 11:16
FEATURES             Location/Qualifiers
     exon            complement(6139..6942)
                     /note="URA3"
     terminator      3890..4222
                     /note="tADH"
     rep_origin      complement(4682..5846)
                     /note="2%B5"
     gene            7372..8234
                     /note="AmpR"
     rep_origin      complement(8402..9055)
                     /note="pUC ORI"
     PCR_primer      complement(4001..4023)
                     /note="tADH Rev"
     misc_feature    507..524
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    459..460
                     /label=mutation
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
     PCR_primer      484..507
                     /pair=""
                     /primer="GAGATATACATATGCGGGGTTCTC"
                     /current=0
     PCR_primer      complement(570..593)
                     /primer="841X"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     promoter        1..452
                     /note="pPMA1"
     PCR_primer      356..382
                     /note=""
     PCR_primer      125..146
                     /note="X150"
     misc_feature    598..602
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    603..1289
                     /note="AFPt9"
     misc_feature    1302..1326
                     /note="attR1"
                     /label=attR1
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    1535..2126
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    1422..1422
                     /label=mutation(2)
                     /ApEinfo_label="mutation"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
     misc_feature    2128..2194
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2127..2127
                     /label=mutation(1)
                     /ApEinfo_label="mutation"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
     misc_feature    2594..2900
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    3045..3064
                     /note="attR2"
                     /label=attR2
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    3756..3785
                     /note="cMyc tag"
     gene            3078..3755
                     /note="TFPt9"
     source          1..9733
                     /dnas_title="pDRFLIP35"
ORIGIN      
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gaTctagAaa TAATTTTGTT TAACTTTAAG
      481 AAGGAGATAT ACATATGCGG GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA
      541 CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT CCGGGCCTCG
      601 AGGTTTCTAA AGGTGAAGAA TTGTTTACGG GCGTCGTCCC GATCCTCGTG GAACTCGACG
      661 GGGATGTTAA CGGGCATAAG TTTTCGGTCA GCGGGGAAGG GGAGGGGGAC GCGACGTATG
      721 GGAAGCTCAC TCTCAAGCTG ATCTGTACGA CGGGGAAACT CCCGGTCCCG TGGCCGACGC
      781 TGGTCACGAC GCTGGGATAC GGGCTCCAAT GCTTTGCGAG GTATCCGGAC CACATGAAAC
      841 AGCATGACTT TTTCAAATCG GCGATGCCGG AGGGATACGT GCAGGAACGG ACGATCTTTT
      901 TCAAAGACGA TGGGAACTAT AAGACGCGGG CGGAAGTCAA GTTTGAAGGG GACACGCTCG
      961 TCAACCGGAT CGAACTCAAG GGGATTGACT TCAAAGAGGA TGGGAACATA CTCGGCCATA
     1021 AGCTCGAATA CAATTACAAC TCGCATAACG TATACATCAC CGCGGATAAG CAAAAGAACG
     1081 GGATCAAAGC CAATTTCAAA ATCCGGCATA ACATAGAGGA TGGGGGGGTC CAACTGGCGG
     1141 ATCACTATCA GCAAAACACG CCGATAGGGG ATGGGCCGGT CCTCCTCCCG GATAACCATT
     1201 ACCTCTCGTA CCAAAGCGCG CTCTCGAAGG ACCCGAATGA GAAACGGGAC CACATGGTTC
     1261 TCCTGGAGTT CGTCACGGCG GCGGGCATAG GTACCGATAT CACAAGTTTG TACAAAAAAG
     1321 CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA
     1381 AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC AcTATGGCGG CCGCATTAGG
     1441 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA
     1501 TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC
     1561 CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC
     1621 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA
     1681 GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC
     1741 TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA
     1801 CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA
     1861 CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA
     1921 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC
     1981 CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC
     2041 CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT
     2101 TCAGGTTCAT CATGCCGTTT GTGATGgGCT TCCATGTCGG CAGAATGCTT AATGAATTAC
     2161 ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT GGATCCGGCT TACTAAAAGC
     2221 CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT ATACTGATAT
     2281 GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC AGCGTATTAC AGTGACAGTT
     2341 GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC GGTCTGGTAA
     2401 GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA GCGGAAAATC
     2461 AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT GCTGACGAGA
     2521 ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAACTTTTGC TGACGAGAAC
     2581 AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT
     2641 GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT
     2701 GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT
     2761 CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT
     2821 CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT
     2881 GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC
     2941 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
     3001 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG
     3061 TGGTGATATC GACTAGTgtg agcaagggcg aggagaccac aatgggcgta atcaagcccg
     3121 acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc gtgatcgagg
     3181 gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag gtgaaggagg
     3241 gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac ggcaacaggg
     3301 ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc cccgagggct
     3361 actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg aagtccgaca
     3421 tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag aacttccccc
     3481 ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc gagaggatgt
     3541 acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg gagggcggcg
     3601 gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg aagctgcccg
     3661 actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac tacaacaagg
     3721 tgaccgttta cgagagcgcc gtggcccgca actccGAACA AAAATTGATA AGTGAGGAAG
     3781 ATTTATAAGG Gccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
     3841 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt
     3901 ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt
     3961 gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac
     4021 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     4081 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaraat tcttattctt
     4141 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     4201 tgaccacacc tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg
     4261 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4321 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4381 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4441 atcggatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     4501 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     4561 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     4621 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     4681 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     4741 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     4801 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     4861 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     4921 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     4981 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     5041 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     5101 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     5161 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     5221 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     5281 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     5341 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     5401 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     5461 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     5521 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     5581 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     5641 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     5701 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     5761 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     5821 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     5881 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     5941 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     6001 atgctatcat ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt
     6061 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     6121 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     6181 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     6241 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     6301 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     6361 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     6421 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     6481 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     6541 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     6601 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     6661 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     6721 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     6781 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     6841 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     6901 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     6961 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     7021 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     7081 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
     7141 aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     7201 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9241 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9301 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9361 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttcctgaaa
     9421 cggagaaaca taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc
     9481 ttttccggta atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc
     9541 agaaataaac ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg
     9601 agaatttcgt aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct
     9661 cgatcgagac cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc
     9721 tgaagaagct ata
//

