LOCUS       pDRFLIP37               9796 bp    DNA     circular     01-MAY-2015
Created: Mittwoch, 30. Mai 2001 11:16
FEATURES             Location/Qualifiers
     exon            complement(6202..7005)
                     /note="URA3"
     terminator      3953..4285
                     /note="tADH"
     rep_origin      complement(4745..5909)
                     /note="2%B5"
     gene            7435..8297
                     /note="AmpR"
     rep_origin      complement(8465..9118)
                     /note="pUC ORI"
     misc_feature    507..524
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    598..602
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    603..1316
                     /note="Citrine"
     misc_feature    1317..1322
                     /note="KpnI"
     misc_feature    3105..3818
                     /note="Cerulean"
     misc_feature    3819..3848
                     /note="cMyc tag"
     promoter        1..452
                     /note="pPMA1"
     misc_feature    1329..1453
                     /note="attR1"
     gene            1562..2153
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2155..2221
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2621..2927
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2967..3091
                     /note="attR2"
ORIGIN      
          1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
         61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
        121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
        181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
        241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
        301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
        361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
        421 aaagaaagaa aaaaaatata ccccagcctc gaTctagAaa TAATTTTGTT TAACTTTAAG
        481 AAGGAGATAT ACATATGCGG GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA
        541 CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT CCGGGCCTCG
        601 AGgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
        661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
        721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
        781 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
        841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
        901 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
        961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
       1021 agcttgagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
       1081 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
       1141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
       1201 acctgagcta ccagtccgcc ctgAGCaaag accccaacga gaagcgcgat cacatggtcc
       1261 tgctggagtt cGTGaccgcc gccgggatca ctctcggcat ggacgagctg tacaagGGTA
       1321 CCGATATCAC AAGTTTGTAC AAAAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
       1381 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
       1441 TCCAGTCAcT ATGGCGGCCG CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC
       1501 GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGAGATTT TCAGGAGCTA AGGAAGCTAA
       1561 AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA
       1621 ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA
       1681 TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT
       1741 TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG
       1801 TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA
       1861 AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA
       1921 TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA
       1981 GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT
       2041 GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG
       2101 CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTTTGTG ATGgGCTTCC
       2161 ATGTCGGCAG AATGCTTAAT GAATTACACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA
       2221 AACGCGTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT GCGTATTTGC GCGCTGATTT
       2281 TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA GAGGTATGCT
       2341 ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT CAAGGCATAT
       2401 ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC CGTCGTCTGC
       2461 GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC CGGTTTATTG
       2521 AAATGAACGG CTCTTTTGCT GACGAGAACA GGGGCTGGTG AAATGCAGTT TAAGGTTTAC
       2581 ACCTATAAAA CTTTTGCTGA CGAGAACAGG GGCTGGTGAA ATGCAGTTTA AGGTTTACAC
       2641 CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC
       2701 GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC
       2761 CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA
       2821 TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA
       2881 AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT
       2941 TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT
       3001 ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG
       3061 TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TGATATCGAC TAGTgtgagc aagggcgagg
       3121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
       3181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
       3241 tcatctgcac caccggtaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
       3301 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
       3361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
       3421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
       3481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
       3541 tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
       3601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
       3661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccG
       3721 CCctgAGCaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcGTGaccg
       3781 ccgccgggat cactctcggc atggacgagc tgtacaagGA ACAAAAATTG ATAAGTGAGG
       3841 AAGATTTATA AGGGccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
       3901 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc
       3961 tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac
       4021 cttgccagaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga
       4081 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
       4141 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgar aattcttatt
       4201 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
       4261 tattgaccac acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac
       4321 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
       4381 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
       4441 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
       4501 ataatcggat cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt
       4561 ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga
       4621 agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt
       4681 gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt
       4741 tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
       4801 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
       4861 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
       4921 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
       4981 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
       5041 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
       5101 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
       5161 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
       5221 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
       5281 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
       5341 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
       5401 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
       5461 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
       5521 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
       5581 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
       5641 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
       5701 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca
       5761 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
       5821 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
       5881 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa
       5941 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt
       6001 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct
       6061 tcaatgctat catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga
       6121 attgggtaat aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc
       6181 atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca
       6241 gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc
       6301 tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg
       6361 ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc
       6421 gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt
       6481 gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt
       6541 gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc
       6601 ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc
       6661 ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc
       6721 agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg
       6781 cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca
       6841 aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa
       6901 tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg
       6961 actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt
       7021 cctgcatgtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
       7081 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
       7141 ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata
       7201 aaaaaaaaat gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc
       7261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
       7321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
       7381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
       7441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
       7501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
       7561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
       7621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
       7681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
       7741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
       7801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
       7861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
       7921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
       7981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
       8041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
       8101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
       8161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
       8221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
       8281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
       8341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
       8401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
       8461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
       8521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
       8581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
       8641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
       8701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
       8761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
       8821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
       8881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
       8941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
       9001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       9061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
       9121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
       9181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       9241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       9301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
       9361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
       9421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttcctg
       9481 aaacggagaa acataaacag gcattgctgg gatcacccat acatcactct gttttgcctg
       9541 accttttccg gtaatttgaa aacaaacccg gtctcgaagc ggagatccgg cgataattac
       9601 cgcagaaata aacccataca cgagacgtag aaccagccgc acatggccgg agaaactcct
       9661 gcgagaattt cgtaaactcg cgcgcattgc atctgtattt cctaatgcgg cacttccagg
       9721 cctcgatcga gaccgtttat ccattgcttt tttgttgtct ttttccctcg ttcacagaaa
       9781 gtctgaagaa gctata
//

