LOCUS       pDRFLIP43               9769 bp    DNA     circular     01-MAY-2015
Created: Mittwoch, 30. Mai 2001 11:16
FEATURES             Location/Qualifiers
     exon            complement(6175..6978)
                     /note="URA3"
     terminator      3926..4258
                     /note="tADH"
     rep_origin      complement(4718..5882)
                     /note="2%B5"
     gene            7408..8270
                     /note="AmpR"
     rep_origin      complement(8438..9091)
                     /note="pUC ORI"
     misc_feature    507..524
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     promoter        1..452
                     /note="pPMA1"
     misc_feature    598..602
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    603..1289
                     /note="edAFPt9"
     misc_feature    1290..1295
                     /note="KpnI"
     misc_feature    1302..1426
                     /note="attR1"
     gene            1535..2126
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2128..2194
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2594..2900
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2940..3064
                     /note="attR2"
     gene            3078..3791
                     /note="edCer"
     misc_feature    3792..3821
                     /note="cMyc tag"
ORIGIN      
          1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
         61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
        121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
        181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
        241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
        301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
        361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
        421 aaagaaagaa aaaaaatata ccccagcctc gaTctagAaa TAATTTTGTT TAACTTTAAG
        481 AAGGAGATAT ACATATGCGG GGTTCTCATC ATCATCATCA TCATGGTATG GCTAGCATGA
        541 CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT CCGGGCCTCG
        601 AGGTTTCTAA AGGTGAAGAA TTGTTTACGG GCGTCGTCCC GATCCTCGTG GAACTCGACG
        661 GGGATGTTAA CGGGCATAAG TTTTCGGTCA GCGGGGAAGG GGAGGGGGAC GCGACGTATG
        721 GGAAGCTCAC TCTCAAGCTG ATCTGTACGA CGGGGAAACT CCCGGTCCCG TGGCCGACGC
        781 TGGTCACGAC GCTGGGATAC GGGCTCCAAT GCTTTGCGAG GTATCCGGAC CACATGAAAC
        841 AGCATGACTT TTTCAAATCG GCGATGCCGG AGGGATACGT GCAGGAACGG ACGATCTTTT
        901 TCAAAGACGA TGGGAACTAT AAGACGCGGG CGGAAGTCAA GTTTGAAGGG GACACGCTCG
        961 TCAACCGGAT CGAACTCAAG GGGATTGACT TCAAAGAGGA TGGGAACATA CTCGGCCATA
       1021 AGCTCGAATA CAATTACAAC TCGCATAACG TATACATCAC CGCGGATAAG CAAAAGAACG
       1081 GGATCAAAGC CAATTTCAAA ATCCGGCATA ACATAGAGGA TGGGGGGGTC CAACTGGCGG
       1141 ATCACTATCA GCAAAACACG CCGATAGGGG ATGGGCCGGT CCTCCTCCCG GATAACCATT
       1201 ACCTCTCGTA CCAAAGCGCG CTCttcAAGG ACCCGAATGA GAAACGGGAC CACATGGTTC
       1261 TCCTGGAGTT CctcACGGCG GCGGGCATAG GTACCGATAT CACAAGTTTG TACAAAAAAG
       1321 CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA
       1381 AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC AcTATGGCGG CCGCATTAGG
       1441 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA
       1501 TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC
       1561 CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC
       1621 TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA
       1681 GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC
       1741 TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA
       1801 CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA
       1861 CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA
       1921 AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC
       1981 CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC
       2041 CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT
       2101 TCAGGTTCAT CATGCCGTTT GTGATGgGCT TCCATGTCGG CAGAATGCTT AATGAATTAC
       2161 ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT GGATCCGGCT TACTAAAAGC
       2221 CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT ATAAGAATAT ATACTGATAT
       2281 GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC AGCGTATTAC AGTGACAGTT
       2341 GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT CAATATCTCC GGTCTGGTAA
       2401 GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA ACGCTGGAAA GCGGAAAATC
       2461 AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA CGGCTCTTTT GCTGACGAGA
       2521 ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA AAACTTTTGC TGACGAGAAC
       2581 AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT
       2641 GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT
       2701 GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT
       2761 CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT
       2821 CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT
       2881 GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC
       2941 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
       3001 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG
       3061 TGGTGATATC GACTAGTgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       3121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
       3181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggt aagctgcccg
       3241 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc
       3301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
       3361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
       3421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
       3481 acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat atcaccgccg
       3541 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
       3601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
       3661 tgcccgacaa ccactacctg agcacccagt ccgccctgTT Caaagacccc aacgagaagc
       3721 gcgatcacat ggtcctgctg gagttcCTGa ccgccgccgg gatcactctc ggcatggacg
       3781 agctgtacaa gGAACAAAAA TTGATAAGTG AGGAAGATTT ATAAGGGccg ggctgcagga
       3841 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
       3901 cctatagtga gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt
       3961 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
       4021 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
       4081 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
       4141 tgactcttag gttttaaaac garaattctt attcttgagt aactctttcc tgtaggtcag
       4201 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
       4261 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
       4321 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
       4381 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
       4441 ctccttacgc atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc
       4501 attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc
       4561 tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg
       4621 agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct
       4681 gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt
       4741 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
       4801 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
       4861 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
       4921 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
       4981 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
       5041 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
       5101 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
       5161 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
       5221 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
       5281 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
       5341 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
       5401 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
       5461 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
       5521 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
       5581 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
       5641 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
       5701 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
       5761 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
       5821 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
       5881 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
       5941 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
       6001 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
       6061 gatcgatccg atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat
       6121 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
       6181 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
       6241 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
       6301 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
       6361 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
       6421 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
       6481 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
       6541 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
       6601 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
       6661 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
       6721 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
       6781 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
       6841 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
       6901 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
       6961 atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg
       7021 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
       7081 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
       7141 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta
       7201 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       7261 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
       7321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
       7381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
       7441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
       7501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
       7561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
       7621 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
       7681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
       7741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
       7801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
       7861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
       7921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
       7981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
       8041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
       8101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
       8161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
       8221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
       8281 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
       8341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
       8401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
       8461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
       8521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
       8581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
       8641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
       8701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
       8761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       8821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
       8881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
       8941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
       9001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
       9061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
       9121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
       9181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
       9241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
       9301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
       9361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
       9421 acagctatga ccatgattac gccaagcttc ctgaaacgga gaaacataaa caggcattgc
       9481 tgggatcacc catacatcac tctgttttgc ctgacctttt ccggtaattt gaaaacaaac
       9541 ccggtctcga agcggagatc cggcgataat taccgcagaa ataaacccat acacgagacg
       9601 tagaaccagc cgcacatggc cggagaaact cctgcgagaa tttcgtaaac tcgcgcgcat
       9661 tgcatctgta tttcctaatg cggcacttcc aggcctcgat cgagaccgtt tatccattgc
       9721 ttttttgttg tctttttccc tcgttcacag aaagtctgaa gaagctata
//

