LOCUS       PMD-19-T                5363 bp    DNA     circular     22-NOV-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|571500692|
COMMENT     VNTDBDATE|571524626|
COMMENT     LSOWNER|
COMMENT     VNTNAME|PMD-19-T|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "PMD-19-T" 0 5363 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AgeI" "KpnI" "SalI" "ScaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 432 3816 1 5363 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 2430 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 413 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3107 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4857 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "P2A" 21 0 0 1712 1777 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1712..1777") 
COMMENT        #5=(CFSignal (CObList) "Gal4-VP16" 21 0 0 1778 2428 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "Gal4-R1" 21 0 0 2108 2130 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "th left arm" 21 0 0 414 1711 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "414..1711") 
COMMENT        #8=(CFSignal (CObList) "th right arm" 21 0 0 2435 3105 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2435..3105") 
COMMENT        #9=(CFSignal (CObList) "th sgRNA target" 21 0 0 641 660 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "PAM" 21 0 0 638 640 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #11=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #12=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "PMD-19-T" 2) 5 "" 
COMMENT                   0 4) 
COMMENT              #13=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'PMD-19-T'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/22/10 02:11\317\302\316\347" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/22/10 08:50\317\302\316\347" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5363 bp" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 22-NOV-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #24=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #25=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #28=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 414 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #29=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 414   End: 1711" 1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   414..1711" 1))) "th left arm") 
COMMENT                            #32=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 638 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 638   End: 640 " 1))) "PAM") 
COMMENT                            #34=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 641 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 641   End: 660 " 1))) 
COMMENT                                 "th sgRNA target") 
COMMENT                            #36=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1712 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1712  End: 1777" 1) 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1712..1777" 1))) "P2A") 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1778 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1778  End: 2428" 1))) 
COMMENT                                 "Gal4-VP16") 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2108 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2108  End: 2130" 1))) "Gal4-R1") 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2435 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2435  End: 3105" 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2435..3105" 1))) "th right arm"))) 
COMMENT                          "Misc. Feature (7 total)"))) "Feature Map") 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #49=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 164556280 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #50=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2430 " 1))))) "AgeI: 1 site") 1 5 
COMMENT                          "ACCGGT" "TGGCCA") 
COMMENT                     #52=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 164556344 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #53=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 413 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
COMMENT                     #55=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 164556440 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #56=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3107 " 1))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
COMMENT                     #58=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 164530296 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #59=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 4857 " 1))))) "ScaI: 1 site") 3 3 
COMMENT                          "AGTACT" "TCATGA"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #61=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #62=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #63=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #64=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #65=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT               (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #66=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT               (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #67=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT               (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #68=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #69=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #70=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #71=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #72=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 
COMMENT               2 "?" 0) 
COMMENT          #73=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) 
COMMENT          #74=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 1 
COMMENT               65535 "@N (@H)" 0) 
COMMENT          #75=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #76=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT               "Fragment of @N" 0) 
COMMENT          #77=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #78=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT               0 0) 
COMMENT          #79=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT               0) 
COMMENT          #80=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT               (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #81=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #82=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #83=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #84=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #85=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #86=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #87=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #88=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 
COMMENT               3 "@T @N " 0) 
COMMENT          #89=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #90=(CWidgetStyle "Annotation" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 
COMMENT               0) 
COMMENT          #91=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #92=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 
COMMENT               0 0 0) 
COMMENT          #93=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #94=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 65535 "@N" 0) 
COMMENT          #95=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT               "@N" 0) 
COMMENT          #96=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT               (LOGBRUSH 0 16777113 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT               (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #97=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT               1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #98=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 1 
COMMENT               1 "@N" 0) 
COMMENT          #99=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #100=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 45.0425 45.0425 39 39 0.1 
COMMENT         -2693) 1 1 0 1 1 
COMMENT        (mapper: 13.1335 -12.4897 45.0425 45.0425 0.01 10 -1 5363 5363 1 0 0) 
COMMENT        #101=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 757089353 100) 
COMMENT              (CObjectList 
COMMENT               #102=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 1919885412 100) 
COMMENT                     (CObjectList 
COMMENT                      #103=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #67# 163827068 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27535 6.27335 1 0.0214037) 0.116972) 
COMMENT                      #104=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #64# 1953789025 100) 
COMMENT                            (LOGPEN 1668573027 1633902945 1668573027) 1952937057 
COMMENT                            (LOGFONT 65 24 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "PMD-19-T" "@N" 1 0 0.871165 0 
COMMENT                            -10 5.3061 1.44308 Nil) 
COMMENT                      #105=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #63# 1966751424 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "5363 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.97497 0.954654 Nil)) (CObjectList)) 
COMMENT               #106=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -431 100) 
COMMENT                     (CObjectList 
COMMENT                      #107=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -824 100) 
COMMENT                            (CObjectList 
COMMENT                             #108=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #66# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 4.1946 4.2718 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #109=(CLabel (CWidget 0 (0 0) 1 2 0 0 #62# 8 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "P2A" "@N" 1 
COMMENT                                   11.8977 -5.22699 0 -10 1.7539 1.17667 #108#) 
COMMENT                             #110=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #66# 1869505646 
COMMENT                                     100) (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 3.43312 4.1946 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #111=(CLabel (CWidget 0 (0 0) 1 2 0 0 #62# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Gal4-VP16" "@N" 
COMMENT                                   1 10.9494 -8.71165 0 -10 4.24044 1.17667 
COMMENT                                   #110#) 
COMMENT                             #112=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #70# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.80859 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #113=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #62# 1667719521 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Gal4-R1" "@N" 1 
COMMENT                                   9.7647 -10.454 0 -10 3.24138 1.17667 #112#) 
COMMENT                             #114=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #66# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 4.2718 5.79009 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #115=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #62# 1734828899 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "th left arm" 
COMMENT                                   "@N" 1 13.7242 3.48466 0 -10 4.04063 1.17667 
COMMENT                                   #114#) 
COMMENT                             #116=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #66# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.64122 3.4261 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #117=(CLabel (CWidget 0 (0 0) 1 2 0 0 #62# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "th right arm" 
COMMENT                                   "@N" 1 -4.55628 -12.1963 0 -10 4.64006 
COMMENT                                   1.17667 #116#) 
COMMENT                             #118=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #70# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.52457 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #119=(CLabel (CWidget 0 (0 0) 1 2 0 0 #62# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "th sgRNA target" "@N" 1 13.4725 6.96932 0 
COMMENT                                   -10 6.39396 1.17667 #118#) 
COMMENT                             #120=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #70# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.52808 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #121=(CLabel (CWidget 0 (0 0) 1 2 0 0 #62# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "PAM" "@N" 1 
COMMENT                                   9.75586 8.71165 0 -10 2.02031 1.17667 #120#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #122=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 1768117093 100) 
COMMENT                     (CObjectList 
COMMENT                      #123=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -980 100) 
COMMENT                            (CObjectList 
COMMENT                             #124=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #69# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 3.43195 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #125=(CLabel (CWidget 0 (0 0) 1 2 0 0 #71# 8 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Age}I (2430)" "@N (@S)" 5 6.35497 
COMMENT                                   -12.1963 0 -10 4.50685 0.954654 #124#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #126=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -980 100) 
COMMENT                            (CObjectList 
COMMENT                             #127=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #69# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.79126 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #128=(CLabel (CWidget 0 (0 0) 1 2 0 0 #71# 429 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Kpn}I (413)" "@N (@S)" 5 8.39007 10.454 
COMMENT                                   0 -10 4.08503 0.954654 #127#)) (CObjectList)) 
COMMENT                      #129=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 1768117093 100) 
COMMENT                            (CObjectList 
COMMENT                             #130=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #69# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.64005 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #131=(CLabel (CWidget 0 (0 0) 1 2 0 0 #71# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Sal}I (3107)" "@N (@S)" 5 -8.44234 
COMMENT                                   -10.454 0 -10 4.15163 0.954654 #130#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #132=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil 1919885412 100) 
COMMENT                            (CObjectList 
COMMENT                             #133=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #69# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 0.593046 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #134=(CLabel (CWidget 0 (0 0) 1 2 0 0 #71# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 43 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Sca}I (4857)" "@N (@S)" 5 -9.39769 
COMMENT                                   10.454 0 -10 4.37365 0.954654 #133#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #135=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1701672291 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #136=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1701672291 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1734435171 
COMMENT       (CStringList) 1952540513 1952539508 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    1712..1777
                     /vntifkey="21"
                     /label=P2A
     misc_feature    414..1711
                     /vntifkey="21"
                     /label=th\left\arm
     misc_feature    2435..3105
                     /vntifkey="21"
                     /label=th\right\arm
     misc_feature    1778..2428
                     /vntifkey="21"
                     /label=Gal4-VP16
     misc_feature    2108..2130
                     /vntifkey="21"
                     /label=Gal4-R1
     misc_feature    641..660
                     /vntifkey="21"
                     /label=th\sgRNA\target
     misc_feature    638..640
                     /vntifkey="21"
                     /label=PAM
BASE COUNT     1575 a      1165 c      1155 g      1468 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgcgatta 
      421 catggcgtag tacgctattt atattctcct ggatattgta taagtgtggt ggtgcttgct 
      481 tctgattttt atataatcca ttaacatatt tatacacata gctaggcctg tttataaact 
      541 tttatgttca aaaaagcttg tacacatgga tgtattaaaa gaacttttat tacctgctgc 
      601 cttttcttcc gtttacacag tttttttttt actttctccc ctttatgagt gtaacaaccc 
      661 ctgggtagtc taggggagtg gggttgcaca cttgaccgaa gattttgttc aaataaacgc 
      721 tcaaagaaaa ttgtactcaa agtaatgacc acatacaaga attgctgtgc aaataaagtg 
      781 atttattaca ggtggtgata agtgaagcag tgagtgagct atttacaata tttacaattc 
      841 acaaccaaaa caaaacataa attagaaaga ctggctatgg gtggcgctaa agcaaagaaa 
      901 caatcaaaac caaacagtct tactaatatc aaatccctgc taatctagtc taataaacaa 
      961 taatgtgaaa aataaatctt gattgtccaa gatgtcttac ctaaactacc taaaaacgtt 
     1021 tggaagtcta taaaagttgt tcagcatttc ttattttgat caatttaaac aattataaag 
     1081 aacacaaaac ataaacattt tgatgttatg atagcaattc caatgtagaa gaatcacttt 
     1141 ctgccctcca tacacaaata aattcagatg gaggctccat ctgaattttg tgcgtcaaca 
     1201 atgacgtcat gtgaaaacaa cctattgcat gcatttggaa atgtacaata acattgcaac 
     1261 aaccaacaca acaacaacca caattataat aataatatca gcgataataa caataataaa 
     1321 aaatatagat tttattctta ttaaaaatag ttttaaaatc acaaagcaaa aagatgcgat 
     1381 aatgagataa aactttctga agttttcaca gtatgtcaac tatcattttt ttgaactagc 
     1441 ttttaaaaaa atgcaattaa ctttttttaa agacatatcc cttaaaacag catgaattga 
     1501 ctaatcacat ttttatttct taaatgtttt ttatttgtta attattattt acagaacata 
     1561 tgtgacccgc atcaagcgac cgttttctgt gcggttcgat ccgtacacgg acagcataga 
     1621 agtgctggac aacccactga agatccagaa aggcctggag accataaaag atgaactgaa 
     1681 gattctcaca gatgccctga atgtgttggc tggatccgga gctactaatt tctccttgct 
     1741 taagcaagct ggtgatgttg aagaaaatcc tggtcctatg aagctactgt cttctatcga 
     1801 acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg 
     1861 cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc 
     1921 gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt 
     1981 tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga 
     2041 tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac 
     2101 agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag 
     2161 tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgtc 
     2221 gagatcgacc ccgggaattc agatctctcg agccgccccc ccgaccgatg tcagcctggg 
     2281 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga 
     2341 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga 
     2401 ctccgccccc tacggcgctc tggatatgac cggttaattc tgcttaatca ttcctctatg 
     2461 cttcatgtgt gacggtctca ttgttatgct gtgcttctat gtctactgta catctgctgc 
     2521 tatagacatt gttgcttcta aaatactgaa tgaaaaactt taaaccttca gcgttaaata 
     2581 atgtatccaa aaatgaaagt tcaccactca aactcacatt acagtagaat atgataatag 
     2641 agatagagaa agagagagac agagagagac ttttatttct gaatgaactt tttttatttt 
     2701 tttttttaag tcatatatga tcaaaattta aaatgataac attttccgct ataattttca 
     2761 ttttggggta aattataact cgtctaaaca gaaatttctg atatgacatt taattaattt 
     2821 atgtacagga tttatttata tttcagatag acgtaccctc attattaaag ctttttaagt 
     2881 caaaaggtat atatgaaata ttaccagagg tcatattcac aatacatatt aagcctaaaa 
     2941 gcagatccta agtttacaat atatatactc caacatacaa ataaacatac aaaatctgtc 
     3001 actgaggtgg tactttttca aaaaatacat ttttgtaaaa taaagatgga cattagaatc 
     3061 tttagggtac acttttgtac cttaaaggta ctaggaggtt cccatgtcga cctgcaggca 
     3121 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     3181 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     3241 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     3301 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     3481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     4021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     4081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     4141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     4201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     4261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     4321 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     4381 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     4441 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     4501 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     4561 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     4621 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     4681 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     4741 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     4801 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     4861 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     4921 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     4981 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     5041 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     5101 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     5161 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     5221 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     5281 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     5341 gcgtatcacg aggccctttc gtc 
//
