LOCUS       FLIP2_delCFPfiller        5431 bp ds-DNA   circular     31-AUG-2010
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    19..37
                     /note="T7 primer"
                     /label=T7 primer
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    111..128
                     /note="His tag"
                     /label=His tag
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    complement(3485..3502)
                     /note="T7 rev"
                     /label=T7 rev
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    3642..3704
                     /note="f1 ori repli"
                     /label=f1 ori repli
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    4195..4281
                     /note="Bla promoter"
                     /label=Bla promoter
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    4283..4903
                     /note="Ampicillin"
                     /label=Ampicillin
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    202..206
                     /label=XhoI
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
     misc_feature    3396..3425
                     /note="cMyc tag"
     misc_feature    894..899
                     /note="KpnI"
     misc_feature    906..1030
                     /note="attR1"
                     /label=attR1
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     gene            1139..1730
                     /note="CmR"
                     /label=CmR
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    1732..1798
                     /note="CmR"
                     /label=CmR(1)
                     /ApEinfo_label="CmR"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     exon            2198..2504
                     /note="ccdB"
                     /label=ccdB
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    2544..2668
                     /note="attR2"
                     /label=attR2
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ff00"
     misc_feature    207..893
                     /note="edAFPt9"
     gene            2682..3395
                     /note="edCer"
ORIGIN      
          1 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA
         61 GAaaTAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC
        121 ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG
        181 ATGACGATAA GGATCCGGGC CTCGAGGTTT CTAAAGGTGA AGAATTGTTT ACGGGCGTCG
        241 TCCCGATCCT CGTGGAACTC GACGGGGATG TTAACGGGCA TAAGTTTTCG GTCAGCGGGG
        301 AAGGGGAGGG GGACGCGACG TATGGGAAGC TCACTCTCAA GCTGATCTGT ACGACGGGGA
        361 AACTCCCGGT CCCGTGGCCG ACGCTGGTCA CGACGCTGGG ATACGGGCTC CAATGCTTTG
        421 CGAGGTATCC GGACCACATG AAACAGCATG ACTTTTTCAA ATCGGCGATG CCGGAGGGAT
        481 ACGTGCAGGA ACGGACGATC TTTTTCAAAG ACGATGGGAA CTATAAGACG CGGGCGGAAG
        541 TCAAGTTTGA AGGGGACACG CTCGTCAACC GGATCGAACT CAAGGGGATT GACTTCAAAG
        601 AGGATGGGAA CATACTCGGC CATAAGCTCG AATACAATTA CAACTCGCAT AACGTATACA
        661 TCACCGCGGA TAAGCAAAAG AACGGGATCA AAGCCAATTT CAAAATCCGG CATAACATAG
        721 AGGATGGGGG GGTCCAACTG GCGGATCACT ATCAGCAAAA CACGCCGATA GGGGATGGGC
        781 CGGTCCTCCT CCCGGATAAC CATTACCTCT CGTACCAAAG CGCGCTCttc AAGGACCCGA
        841 ATGAGAAACG GGACCACATG GTTCTCCTGG AGTTCctcAC GGCGGCGGGC ATAGGTACCG
        901 ATATCACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA
        961 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC
       1021 AGTCAcTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA
       1081 TAATGTGTGG ATTTTGAGTT AGGATCCGTC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT
       1141 GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA
       1201 TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT
       1261 TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA
       1321 CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA
       1381 GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC
       1441 GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC
       1501 GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA
       1561 TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC
       1621 CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA
       1681 CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTTTGTGATG gGCTTCCATG
       1741 TCGGCAGAAT GCTTAATGAA TTACACAGTA CTGCGATGAG TGGCAGGGCG GGGCGTAAAC
       1801 GCGTGGATCC GGCTTACTAA AAGCCAGATA ACAGTATGCG TATTTGCGCG CTGATTTTTG
       1861 CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG TCAAAAAGAG GTATGCTATG
       1921 AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC AGTTGCTCAA GGCATATATG
       1981 ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA TGAAGCCCGT CGTCTGCGTG
       2041 CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA GGTCGCCCGG TTTATTGAAA
       2101 TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA TGCAGTTTAA GGTTTACACC
       2161 TATAAAACTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG TTTACACCTA
       2221 TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA TTGACACGCC
       2281 CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA AAGTCTCCCG
       2341 TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA CCACCGATAT
       2401 GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC ACCGCGAAAA
       2461 TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG GCTCCCTTAT
       2521 ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG
       2581 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT
       2641 CTCGTTCAGC TTTCTTGTAC AAAGTGGTGA TATCGACTAG Tgtgagcaag ggcgaggagc
       2701 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
       2761 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
       2821 tctgcaccac cggtaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
       2881 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
       2941 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
       3001 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
       3061 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca
       3121 gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
       3181 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
       3241 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
       3301 tgTTCaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc CTGaccgccg
       3361 ccgggatcac tctcggcatg gacgagctgt acaagGAACA AAAATTGATA AGTGAGGAAG
       3421 ATTTATAAGG GCCCGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG
       3481 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT
       3541 TTTTGCTGAA AGGAGGAACT ATATCCGGAT CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
       3601 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC
       3661 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
       3721 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
       3781 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGAGCTTTAC GGCACCTCGA
       3841 CCGCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
       3901 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
       3961 AACAACACTC AACCCTATCG CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
       4021 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAATATTT AACGCGAATT TTAACAAAAT
       4081 ATTAACGTTT ACAATTTCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT
       4141 CACACCGCAT ACAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
       4201 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
       4261 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
       4321 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
       4381 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
       4441 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
       4501 GCTATGTGAT ACACTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
       4561 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
       4621 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
       4681 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
       4741 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
       4801 CGACGAGAGT GACACCACGA TGCCTGTAGC AATGCCAACA ACGTTGCGCA AACTATTAAC
       4861 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGAATGG AGGCGGATAA
       4921 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
       4981 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCG
       5041 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
       5101 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
       5161 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
       5221 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
       5281 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
       5341 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
       5401 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
       5461 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
       5521 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
       5581 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
       5641 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
       5701 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
       5761 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
       5821 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
       5881 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGGCTT
       5941 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
       6001 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
       6061 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
       6121 GCCGATTCAT TAATGCAGg
//

