LOCUS       MSP712                  8068 bp    DNA     circular UNA 22-AUG-2002
DEFINITION  pACYCDUET-1.
ACCESSION   urn.local...h-4oqvoi6
VERSION     urn.local...h-4oqvoi6
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     protein_bind    5..25
                     /vntifkey=31
                     /label="lacO"
     RBS             58..63
                     /vntifkey=32
                     /label="RBS-1"
     CDS             71..4174
                     /%_Identity=100
                     /Motif="ATGGATAAAAAGTATTCTATTGGTTTAGACATCGGCACTAATTCCGTTGG
                     ATGGGCTGTCATAACCGATGAATACAAAGTACCTTCAAAGAAATTTAAGGTGTTGGGG
                     AACACAGACCGTCATTCGATTAAAAAGAATCTTATCGGTGCCCTCCTATTCGATAGTG
                     GCGAAACGGCAGAGGCGACTCGCCTGAAACGAACCGCTCGGAGAAGGTATACACGTCG
                     CAAGAACCGAATATGTTACTTACAAGAAATTTTTAGCAATGAGATGGCCAAAGTTGAC
                     GATTCTTTCTTTCACCGTTTGGAAGAGTCCTTCCTTGTCGAAGAGGACAAGAAACATG
                     AACGGCACCCCATCTTTGGAAACATAGTAGATGAGGTGGCATATCATGAAAAGTACCC
                     AACGATTTATCACCTCAGAAAAAAGCTAGTTGACTCAACTGATAAAGCGGACCTGAGG
                     TTAATCTACTTGGCTCTTGCCCATATGATAAAGTTCCGTGGGCACTTTCTCATTGAGG
                     GTGATCTAAATCCGGACAACTCGGATGTCGACAAACTGTTCATCCAGTTAGTACAAAC
                     CTATAATCAGTTGTTTGAAGAGAACCCTATAAATGCAAGTGGCGTGGATGCGAAGGCT
                     ATTCTTAGCGCCCGCCTCTCTAAATCCCGACGGCTAGAAAACCTGATCGCACAATTAC
                     CCGGAGAGAAGAAAAATGGGTTGTTCGGTAACCTTATAGCGCTCTCACTAGGCCTGAC
                     ACCAAATTTTAAGTCGAACTTCGACTTAGCTGAAGATGCCAAATTGCAGCTTAGTAAG
                     GACACGTACGATGACGATCTCGACAATCTACTGGCACAAATTGGAGATCAGTATGCGG
                     ACTTATTTTTGGCTGCCAAAAACCTTAGCGATGCAATCCTCCTATCTGACATACTGAG
                     AGTTAATACTGAGATTACCAAGGCGCCGTTATCCGCTTCAATGATCAAAAGGTACGAT
                     GAACATCACCAAGACTTGACACTTCTCAAGGCCCTAGTCCGTCAGCAACTGCCTGAGA
                     AATATAAGGAAATATTCTTTGATCAGTCGAAAAACGGGTACGCAGGTTATATTGACGG
                     CGGAGCGAGTCAAGAGGAATTCTACAAGTTTATCAAACCCATATTAGAGAAGATGGAT
                     GGGACGGAAGAGTTGCTTGTAAAACTCAATCGCGAAGATCTACTGCGAAAGCAGCGGA
                     CTTTCGACAACGGTAGCATTCCACATCAAATCCACTTAGGCGAATTGCATGCTATACT
                     TAGAAGGCAGGAGGATTTTTATCCGTTCCTCAAAGACAATCGTGAAAAGATTGAGAAA
                     ATCCTAACCTTTCGCATACCTTACTATGTGGGACCCCTGGCCCGAGGGAACTCTCGGT
                     TCGCATGGATGACAAGAAAGTCCGAAGAAACGATTACTCCATGGAATTTTGAGGAAGT
                     TGTCGATAAAGGTGCGTCAGCTCAATCGTTCATCGAGAGGATGACCAACTTTGACAAG
                     AATTTACCGAACGAAAAAGTATTGCCTAAGCACAGTTTACTTTACGAGTATTTCACAG
                     TGTACAATGAACTCACGAAAGTTAAGTATGTCACTGAGGGCATGCGTAAACCCGCCTT
                     TCTAAGCGGAGAACAGAAGAAAGCAATAGTAGATCTGTTATTCAAGACCAACCGCAAA
                     GTGACAGTTAAGCAATTGAAAGAGGACTACTTTAAGAAAATTGAATGCTTCGATTCTG
                     TCGAGATCTCCGGGGTAGAAGATCGATTTAATGCGTCACTTGGTACGTATCATGACCT
                     CCTAAAGATAATTAAAGATAAGGACTTCCTGGATAACGAAGAGAATGAAGATATCTTA
                     GAAGATATAGTGTTGACTCTTACCCTCTTTGAAGATCGGGAAATGATTGAGGAAAGAC
                     TAAAAACATACGCTCACCTGTTCGACGATAAGGTTATGAAACAGTTAAAGAGGCGTCG
                     CTATACGGGCTGGGGACGATTGTCGCGGAAACTTATCAACGGGATAAGAGACAAGCAA
                     AGTGGTAAAACTATTCTCGATTTTCTAAAGAGCGACGGCTTCGCCAATAGGAACTTTA
                     TGCAGCTGATCCATGATGACTCTTTAACCTTCAAAGAGGATATACAAAAGGCACAGGT
                     TTCCGGACAAGGGGACTCATTGCACGAACATATTGCGAATCTTGCTGGTTCGCCAGCC
                     ATCAAAAAGGGCATACTCCAGACAGTCAAAGTAGTGGATGAGCTAGTTAAGGTCATGG
                     GACGTCACAAACCGGAAAACATTGTAATCGAGATGGCACGCGAAAATCAAACGACTCA
                     GAAGGGGCAAAAAAACAGTCGAGAGCGGATGAAGAGAATAGAAGAGGGTATTAAAGAA
                     CTGGGCAGCCAGATCTTAAAGGAGCATCCTGTGGAAAATACCCAATTGCAGAACGAGA
                     AACTTTACCTCTATTACCTACAAAATGGAAGGGACATGTATGTTGATCAGGAACTGGA
                     CATAAACCGTTTATCTGATTACGACGTCGATCACATTGTACCCCAATCCTTTTTGAAG
                     GACGATTCAATCGACAATAAAGTGCTTACACGCTCGGATAAGAACCGAGGGAAAAGTG
                     ACAATGTTCCAAGCGAGGAAGTCGTAAAGAAAATGAAGAACTATTGGCGGCAGCTCCT
                     AAATGCGAAACTGATAACGCAAAGAAAGTTCGATAACTTAACTAAAGCTGAGAGGGGT
                     GGCTTGTCTGAACTTGACAAGGCCGGATTTATTAAACGTCAGCTCGTGGAAACCCGCC
                     AAATCACAAAGCATGTTGCACAGATACTAGATTCCCGAATGAATACGAAATACGACGA
                     GAACGATAAGCTGATTCGGGAAGTCAAAGTAATCACTTTAAAGTCAAAATTGGTGTCG
                     GACTTCAGAAAGGATTTTCAATTCTATAAAGTTAGGGAGATAAATAACTACCACCATG
                     CGCACGACGCTTATCTTAATGCCGTCGTAGGGACCGCACTCATTAAGAAATACCCGAA
                     GCTAGAAAGTGAGTTTGTGTATGGTGATTACAAAGTTTATGACGTCCGTAAGATGATC
                     GCGAAAAGCGAACAGGAGATAGGCAAGGCTACAGCCAAATACTTCTTTTATTCTAACA
                     TTATGAATTTCTTTAAGACGGAAATCACTCTGGCAAACGGAGAGATACGCAAACGACC
                     TTTAATTGAAACCAATGGGGAGACAGGTGAAATCGTATGGGATAAGGGCCGGGACTTC
                     GCGACGGTGAGAAAAGTTTTGTCCATGCCCCAAGTCAACATAGTAAAGAAAACTGAGG
                     TGCAGACCGGAGGGTTTTCAAAGGAATCGATTCTTCCAAAAAGGAATAGTGATAAGCT
                     CATCGCTCGTAAAAAGGACTGGGACCCGAAAAAGTACGGTGGCTTCGATAGCCCTACA
                     GTTGCCTATTCTGTCCTAGTAGTGGCAAAAGTTGAGAAGGGAAAATCCAAGAAACTGA
                     AGTCAGTCAAAGAATTATTGGGGATAACGATTATGGAGCGCTCGTCTTTTGAAAAGAA
                     CCCCATCGACTTCCTTGAGGCGAAAGGTTACAAGGAAGTAAAAAAGGATCTCATAATT
                     AAACTACCAAAGTATAGTCTGTTTGAGTTAGAAAATGGCCGAAAACGGATGTTGGCTA
                     GCGCCGGAGAGCTTCAAAAGGGGAACGAACTCGCACTACCGTCTAAATACGTGAATTT
                     CCTGTATTTAGCGTCCCATTACGAGAAGTTGAAAGGTTCACCTGAAGATAACGAACAG
                     AAGCAACTTTTTGTTGAGCAGCACAAACATTATCTCGACGAAATCATAGAGCAAATTT
                     CGGAATTCAGTAAGAGAGTCATCCTAGCTGATGCCAATCTGGACAAAGTATTAAGCGC
                     ATACAACAAGCACAGGGATAAACCCATACGTGAGCAGGCGGAAAATATTATCCATTTG
                     TTTACTCTTACCAACCTCGGCGCTCCAGCCGCATTCAAGTATTTTGACACAACGATAG
                     ATCGCAAACGATACACTTCTACCAAGGAGGTGCTAGACGCGACACTGATTCACCAATC
                     CATCACGGGATTATATGAAACTCGGATAGATTTGTCACAGCTTGGGGGTGACGGATCC
                     CCCAAGAAGAAGAGGAAAGTCTCGAGCGACTACAAAGACCATGACGGTGATTATAAAG
                     ATCATGACATCGATTACAAGGATGACGATGACAAGTGA"
                     /annotation_group="CRISPR_motif: 1,769 -> 5,974"
                     /modified_by="User"
                     /label="Sp-dCas9"
     orf             4181..4201
                     /created_by="User"
                     /Transferred_From="BPK2101, BPK2139, BPK764 "
                     /Transferred_Similarity="100.00%"
                     /label="SV40 NLS"
     orf             4208..4273
                     /created_by="User"
                     /Transferred_From="BPK2101, BPK2139, BPK764 "
                     /Transferred_Similarity="100.00%"
                     /label="3xFLAG"
     primer          4318..4337
                     /vntifkey=27
                     /label="DuetUP2\Primer\#71180-3"
     primer          complement(4318..4337)
                     /vntifkey=27
                     /label="DuetDOWN1\Primer\#71179-3"
     promoter        4343..4359
                     /vntifkey=30
                     /label="T7\promoter"
     misc_binding    4362..4429
                     /created_by="User"
                     /modified_by="User"
                     /label="BsaI stuffer cassette"
     misc_rna        4430..4506
                     /created_by="User"
                     /Transferred_From="BPK764 "
                     /Transferred_Similarity="100.00%"
                     /label="SpCas9 gRNA"
     misc_difference 4431..4434
                     /created_by="User"
                     /label="different 3' overhang than BPK764"
     primer          complement(4507..4525)
                     /vntifkey=27
                     /label="T7\Terminator\Primer\#69337-3"
     terminator      4521..4568
                     /vntifkey=43
                     /label="T7\Terminator"
     CDS             complement(4789..5448)
                     /vntifkey=4
                     /label="CAT"
     rep_origin      5810..6722
                     /vntifkey=33
                     /label="P15A\ori"
     CDS             complement(6846..7928)
                     /vntifkey=4
                     /note="lacI gene"
                     /label="lacI"
     primer          8009..8028
                     /vntifkey=27
                     /label="ACYCDuetUP1\Primer\#71178-3"
     promoter        8052..8068
                     /vntifkey=30
                     /label="T7\Promoter"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggataaaa agtattctat tggtttagcc atcggcacta attccgttgg
      121 atgggctgtc ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa
      181 cacagaccgt cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga
      241 aacggcagag gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa
      301 ccgaatatgt tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt
      361 ctttcaccgt ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc
      421 catctttgga aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca
      481 cctcagaaaa aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc
      541 tcttgcccat atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga
      601 caactcggat gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga
      661 agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc
      721 taaatcccga cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt
      781 gttcggtaac cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga
      841 cttagctgaa gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa
      901 tctactggca caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag
      961 cgatgcaatc ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt
     1021 atccgcttca atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc
     1081 cctagtccgt cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa
     1141 cgggtacgca ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa
     1201 acccatatta gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga
     1261 tctactgcga aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg
     1321 cgaattgcat gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg
     1381 tgaaaagatt gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg
     1441 agggaactct cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccctggaa
     1501 ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa
     1561 ctttgacaag aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta
     1621 tttcacagtg tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc
     1681 cgcctttcta agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg
     1741 caaagtgaca gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc
     1801 tgtcgagatc tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct
     1861 cctaaagata attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga
     1921 agatatagtg ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa
     1981 aacatacgct cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac
     2041 gggctgggga cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa
     2101 aactattctc gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat
     2161 ccatgatgac tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg
     2221 ggactcattg cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat
     2281 actccagaca gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga
     2341 aaacattgta atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag
     2401 tcgagagcgg atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa
     2461 ggagcatcct gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca
     2521 aaatggaagg gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga
     2581 cgtcgatgcc attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct
     2641 tacacgctcg gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa
     2701 gaaaatgaag aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt
     2761 cgataactta actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat
     2821 taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc
     2881 ccgaatgaat acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac
     2941 tttaaagtca aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga
     3001 gataaataac taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact
     3061 cattaagaaa tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga
     3121 cgtccgtaag atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt
     3181 cttttattct aacattatga atttctttaa gacggaaatc actctggcaa acggagagat
     3241 acgcaaacga cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg
     3301 ccgggacttc gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa
     3361 aactgaggtg cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga
     3421 taagctcatc gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc
     3481 tacagttgcc tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact
     3541 gaagtcagtc aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa
     3601 ccccatcgac ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa
     3661 actaccaaag tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc
     3721 cggagagctt caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta
     3781 tttagcgtcc cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact
     3841 ttttgttgag cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag
     3901 taagagagtc atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca
     3961 cagggataaa cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa
     4021 cctcggcgct ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac
     4081 ttctaccaag gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga
     4141 aactcggata gatttgtcac agcttggggg tgacggatcc cccaagaaga agaggaaagt
     4201 ctcgagcgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     4261 tgacgatgac aagtgaagcg gccgcataat gcttaagtcg aacagaaagt aatcgtattg
     4321 tacacggccg cataatcgaa attaatacga ctcactatag ggagacccat gccatagcgt
     4381 tgttcggaat atgaattttt gaacagattc accaacacct agtggtctcg ttttagagct
     4441 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4501 ggtgctccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     4561 gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca
     4621 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     4681 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
     4741 ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     4801 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     4861 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     4921 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     4981 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     5041 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     5101 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     5161 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     5221 tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     5281 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     5341 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     5401 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     5461 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     5521 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     5581 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     5641 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
     5701 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
     5761 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga
     5821 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     5881 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     5941 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     6001 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     6061 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     6121 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg
     6181 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     6241 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     6301 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     6361 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     6421 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     6481 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     6541 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     6601 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     6661 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     6721 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     6781 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     6841 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     6961 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     7021 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     7081 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     7141 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     7201 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     7261 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     7321 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     7381 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7441 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7501 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7561 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     7621 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     7681 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     7741 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     7801 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     7861 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     7921 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     7981 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     8041 cattaggaaa ttaatacgac tcactata
//
