LOCUS BPK1520 2280 bp DNA circular 20-MAY-2013 DEFINITION . ACCESSION urn.local...b-4oqvoi1 KEYWORDS . SOURCE ORGANISM . COMMENT Serial Cloner Genbank Format SerialCloner_Type=DNA SerialCloner_Comments=[DEFINITION]: - [LOCUS]: pUC19-U6-+103gRNA.seq SerialCloner_Ends=0,0,,0,. FEATURES Location/Qualifiers promoter 13..330 /SerialCloner_Color="&h0019C0" /SerialCloner_Show="True" /SerialCloner_Protect="True" /SerialCloner_Arrow="True" /modified_by="User" /label="U6 promoter" misc_feature complement(332..337) /Recognition_pattern="CGTCTC(1/5)" /annotation_group="CGTCTC" /label="BsmBI" /note="Geneious type: restriction site" misc_binding 332..350 /created_by="User" /label="BsmBI stuffer cassette" misc_feature 345..350 /Recognition_pattern="CGTCTC(1/5)" /annotation_group="CGTCTC" /label="BsmBI" /note="Geneious type: restriction site" misc_RNA 352..434 /SerialCloner_Color="&hC00D00" /SerialCloner_Show="True" /SerialCloner_Protect="True" /SerialCloner_Arrow="True" /modified_by="User" /label="SpgRNA" rep_origin complement(472..1145) /Transferred_From="pCRII-TOPO" /Transferred_Similarity="100.00%" /modified_by="User" /label="pUC origin" CDS complement(1290..2150) /created_by="User" /modified_by="User" /label="ampR" promoter complement(2192..2220) /Imported_from="Pla sMapper" /Transferred_From="amp prom" /Transferred_Similarity="100.00%" /modified_by="User" /label="amp promoter" ORIGIN 1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc 61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata 121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta 181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta 241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct 301 ttatatatct tgtggaaagg acgaaacacc ggagacgatt aatgcgtctc cgttttagag 361 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag 421 tcggtgcttt ttttaagctt gggccgctcg aggtacctct ctacatatga catgtgagca 481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 1021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 1081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 1141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 1321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 1441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 1801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 2041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 2101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 2161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 2221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct //