LOCUS       BPK2301                 2329 bp    DNA     circular     20-MAY-2013
DEFINITION  .
ACCESSION   urn.local...e-4oqvoi4
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA
            SerialCloner_Comments=[DEFINITION]: - [LOCUS]:
            pUC19-U6-+103gRNA.seq SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     promoter        13..330
                     /SerialCloner_Color="&h0019C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /modified_by="User"
                     /label="U6 promoter"
     misc_binding    332..350
                     /created_by="User"
                     /Transferred_From="BPK1520"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="BsmBI stuffer cassette"
     misc_feature    complement(332..337)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    345..350
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_RNA        352..483
                     /created_by="User"
                     /modified_by="User"
                     /label="St1gRNA"
     rep_origin      complement(521..1194)
                     /created_by="User"
                     /label="pUC origin"
     cds             complement(1339..2199)
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="BPK1520"
                     /Transferred_Similarity="100.00%"
                     /label="ampR"
     promoter        complement(2241..2269)
                     /created_by="User"
                     /label="amp promoter"
ORIGIN      
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgagacgatt aatgcgtctc ggtttttgta
      361 ctctcaagat ttaagtaact gtacaacgaa acttacacag ttacttaaat cttgcagaag
      421 ctacaaagat aaggcttcat gccgaaatca acaccctgtc attttatggc agggtgtttt
      481 tttaagcttg ggccgctcga ggtacctctc tacatatgac atgtgagcaa aaggccagca
      541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacct
//
