LOCUS       130_pSKB3/LUMP-P.leioghnati    5878 bp      DNA     circular   5/11/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=protein before TEV cleavage: - MW: 22976.9 - pI: 4.78 - AA: 
            211 - Ext coeff: 11460 -  -  - protein after TEV cleavage: -
             MW: 20191 - pI: 4.51 - AA: 188 - Ext coeff: 8480 -  -  - 
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             4835..4852
                     /label=6His
                     /SerialCloner_Color=&hDB0D15
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             4874..4894
                     /label=TEV site
                     /SerialCloner_Color=&hF1881F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     4736..4755
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      1227..1855
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5647..209)
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      5642..219
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    4755..4777
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(315..1127)
                     /label=KanR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(3267..4358)
                     /label=lacI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4898..5455
                     /label=LUMP-P.Leioghnati
                     /SerialCloner_Color=&h1A6AC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MFRGIVQGRGVIRSISKSEDSQRHGIAFPEGMFQLVDVDTVMLVNGCSLTVVRILGDMVYFDIDQALGTTTFDGLKEGDQVNLEIHPKFGEVVGRGGLTGNIKGTALVAAIEENDAGFSVLIDIPKGLAENLTVKDDIGIDGISLPITDMSDSIITLNYSRDLLASTNIASLAKDVKVNVEILNEW" \ 
ORIGIN
        1 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
       61 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      181 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa 
      241 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
      301 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca 
      361 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg 
      421 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca 
      481 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca 
      541 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt 
      601 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc 
      661 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa 
      721 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct 
      781 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt 
      841 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc 
      901 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca 
      961 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct 
     1021 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa 
     1081 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta 
     1141 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt 
     1201 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     1261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     1321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     1381 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     1441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     1501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     1561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     1621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     1681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     1741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     1801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     1861 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     1921 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     1981 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 
     2041 ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc 
     2101 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 
     2161 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 
     2221 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 
     2281 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg 
     2341 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta 
     2401 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg 
     2461 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga 
     2521 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg 
     2581 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc 
     2641 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc 
     2701 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac 
     2761 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc 
     2821 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc 
     2881 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg 
     2941 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag 
     3001 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc 
     3061 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca 
     3121 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg 
     3181 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 
     3241 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     3301 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc 
     3361 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 
     3421 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 
     3481 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact 
     3541 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc 
     3601 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc 
     3661 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 
     3721 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 
     3781 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 
     3841 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 
     3901 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 
     3961 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 
     4021 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 
     4081 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 
     4141 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 
     4201 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 
     4261 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 
     4321 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 
     4381 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 
     4441 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag 
     4501 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac 
     4561 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg 
     4621 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 
     4681 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata 
     4741 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aaTTTTGTTT 
     4801 AACTTTAAGA AGGAGATATA CCATGGGCAG CAGCCATCAT CATCATCATC ACGATTACGA 
     4861 TATCCCAACG ACCGAAAACC TTTACTTCCA GGGCCATATG TTTAGAGGTA TTGTTCAAGG 
     4921 TCGTGGTGTT ATTCGATCCA TTTCAAAAAG TGAAGATAGT CAGCGACATG GGATTGCATT 
     4981 CCCTGAGGGT ATGTTTCAAC TTGTTGATGT AGATACTGTA ATGTTAGTAA ATGGTTGTTC 
     5041 ATTGACAGTT GTCCGTATTT TAGGCGATAT GGTGTACTTT GATATCGACC AAGCATTGGG 
     5101 TACGACAACA TTTGATGGAT TAAAAGAAGG CGATCAGGTA AACCTAGAGA TCCACCCTAA 
     5161 ATTTGGTGAA GTGGTTGGCC GTGGTGGATT AACAGGAAAT ATTAAAGGCA CTGCGCTGGT 
     5221 CGCAGCAATA GAAGAGAATG ACGCAGGTTT TTCTGTACTG ATTGATATCC CTAAAGGGTT 
     5281 AGCTGAAAAC TTAACAGTGA AAGATGATAT TGGAATTGAT GGGATCTCTT TGCCTATCAC 
     5341 TGATATGTCA GACAGCATTA TTACATTGAA TTACTCGCGT GATTTGTTAG CGAGTACTAA 
     5401 CATTGCATCG TTAGCAAAAG ATGTGAAAGT GAACGTTGAA ATTTTAAATG AATGGTAGGC 
     5461 GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA 
     5521 GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 
     5581 TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT TGGCGAATGG 
     5641 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     5701 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     5761 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     5821 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTG
//