LOCUS       C234_pRhl17tet_A        5099 bp ds-DNA     linear       18-OCT-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2054..2148
                     /label=B0014terminator
                     /ApEinfo_fwdcolor=#808040
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3235..4109)
                     /label=SpecR: according to BBa_J23012
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#00ff40
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(866..871)
                     /label=SphI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(800..865)
                     /label=BCD
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1307..1323)
                     /label=TetO2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1903..1908
                     /label=XhoI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4373..4918
                     /label=p15A
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3229..3234
                     /label=NheI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(790..795)
                     /label=SpeI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1338..1902
                     /label=Spacer from T7
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(873..891)
                     /label=TetO2(1)
                     /ApEinfo_label=TetO2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2263..3141
                     /label=araC
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(922..927)
                     /label=-35
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2212..2261
                     /label=araC Promoter
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5094..5099
                     /label=SacI
                     /ApEinfo_fwdcolor=#f5010a
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(928..1013)
                     /label=pCin
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4..789)
                     /label=AiiA from  BT ssra
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(899..904)
                     /label=-10
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2153..2160
                     /label=NotI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(905..921)
                     /label=TetO2(2)
                     /ApEinfo_label=TetO2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(977..1013)
                     /label=Another binding site?
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#80ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ttattacgct gcaagggcgt aattttcgtc gttcgctgct atatattccg ggaacactct
       61 acaactcttt tcctgctcta tatcatgacc aaagaaaata attggtttct cttttttcac
      121 aacttctttt aaacgtttaa ttgaagataa agctaattct ggatcaaatc ctgcgaacgg
      181 cacttcatct tcaaaattct ctttcgtgta cgatgcatca atcgttaata aaactgaacc
      241 ggattgctcc gtctcaatga atagcgactg atggcctgga gaatgacctg gcgtatacaa
      301 taattgaaca cctggtacca cttcataatc cccttcaata attttgtagt tcaaatgcgg
      361 taatatacat tctttcatat attcttctct atgaagtgct gcctcatatt ccgttcgctg
      421 cacaataatt ggtgtatttg taaaagcacc gtttcctcct gcatgatcaa aatgtaagtg
      481 agaactaata atatataaaa ggtcgtccgg ctcataccct acacgcttta atatattcac
      541 gattctatct tcttcagtca ttttcggtaa gatctgtcct tcaacaaatg taccgttaaa
      601 aagcccttca ttattaactg cactttctgg catacctgtg tctactaaaa taggaccttc
      661 ttccgtctcc aaaagataac accacaccgg caagtttaat agtttccccg gtgttaacgc
      721 actgttaaca gacgaatgat ccaacatgca acgacctgct gggatgaaat aaagtttctt
      781 tactgtcata ctagtatttc tccttagcat gattaagatg tttcagtacg aaaattgctt
      841 tcattgttga tctccttttt aagtggcatg catctctatc actgataggg atGTGCTCAT
      901 TATCtctatc actgataggg aAGTAAAttc cctcagatag gcccccccag atgaggggga
      961 tcagagcatg tcgcgcaaaa gtgtgcagcg gttttgcgat aacgacatgc gccacgcaca
     1021 agagttccgt agctgttcaa gtttgtgttt caactgttct cgtcgtttcc gcaacaagtc
     1081 ctcttcagaa atgagctttt gctcctctgc ttggacggac aggatgtatg ctgtggcttt
     1141 tttaaggata actaccttgg gggccttttc attgttttcc aactccggga tctggtcacg
     1201 cagggcaaaa aagctccgtt ttagctcgtt cctcctctgg cgctccaaga cgttgtgtgt
     1261 tcgcctcttg acattctcct cggtgtccga gggccctgtg tgaatctcta tcactgatag
     1321 ggatccacac agacgtctta acttgcttcc tagtgaaacc gttcaggaca tctacgggat
     1381 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga
     1441 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac
     1501 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc
     1561 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga
     1621 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc
     1681 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt
     1741 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa
     1801 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt
     1861 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttCTCGAGta ataaAAGCTT
     1921 cccctacgtg ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga
     1981 tactatgact gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt
     2041 ccgcaccgct gtctcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     2101 agagagaata taaaaagcca gattattaat ccggcttttt tattatttcc tagcggccgc
     2161 ggtttggtta gcgagaagag ccagtaaaag acgcagtgac ggcaatgtct gatgcaatat
     2221 ggacaattgg tttcttctct gaatggcggg agtatgaaaa gtatggctga agcgcaaaat
     2281 gatcccctgc tgccgggata ctcgtttaat gcccatctgg tggcgggttt aacgccgatt
     2341 gaggccaacg gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt
     2401 ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtttgc
     2461 cgaccgggtg atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg
     2521 gaggctcgcg aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa
     2581 tggcttaact ggccgtcaat atttgccaat acggggttct ttcgcccgga tgaagcgcac
     2641 cagccgcatt tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc
     2701 tattcggagc tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg
     2761 attaacgagt cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc
     2821 agcgatcacc tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcttg
     2881 tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg
     2941 cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga gcaccacccg gatgcctatc
     3001 gccaccgtcg gtcgcaatgt tggttttgac gatcaactct atttctcgcg ggtatttaaa
     3061 aaatgcaccg gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat
     3121 gtagccgtca agttgtcata attggtaacg aatcagacaa ttgacggctt gacggagtag
     3181 catagggttt gcagaatccc tgcttcgtcc atttgacagg cacattatGC TAGCGATATC
     3241 CGTCGGCTTG AACGAATTGT TAGACATTAT TTGCCGACTA CCTTGGTGAT CTCGCCTTTC
     3301 ACGTAGTGGA CAAATTCTTC CAACTGATCT GCGCGCGAGG CCAAGCGATC TTCTTCTTGT
     3361 CCAAGATAAG CCTGTCTAGC TTCAAGTATG ACGGGCTGAT ACTGGGCCGG CAGGCGCTCC
     3421 ATTGCCCAGT CGGCAGCGAC ATCCTTCGGC GCGATTTTGC CGGTTACTGC GCTGTACCAA
     3481 ATGCGGGACA ACGTAAGCAC TACATTTCGC TCATCGCCAG CCCAGTCGGG CGGCGAGTTC
     3541 CATAGCGTTA AGGTTTCATT TAGCGCCTCA AATAGATCCT GTTCAGGAAC CGGATCAAAG
     3601 AGTTCCTCCG CCGCTGGACC TACCAAGGCA ACGCTATGTT CTCTTGCTTT TGTCAGCAAG
     3661 ATAGCCAGAT CAATGTCGTA CGTGGCTGGC TCGAAGATAC CTGCAAGAAT GTCATTGCGC
     3721 TGCCATTCTC CAAATTGCAG TTCGCGCTTA GCTGGATAAC GCCACGGAAT GATGTCGTCG
     3781 TGCACAACAA TGGTGACTTC TACAGCGCGG AGAATCTCGC TCTCTCCAGG GGAAGCCGAA
     3841 GTTTCCAAAA GGTCGTTGAT CAAAGCTCGC CGCGTTGTTT CATCAAGCCT TACGGTCACC
     3901 GTAACCAGCA AATCAATATC ACTGTGTGGC TTCAGGCCGC CATCCACTGC GGAGCCGTAC
     3961 AAATGTACGG CCAGCAACGT CGGTTCGAGA TGGCGCTCGA TGACGCCAAC TACCTCTGAT
     4021 AGTTGAGTCG ATACTTCGGC GATCACCGCT TCCCTCATGA TGTTTAACTT TGTTTTAGGG
     4081 CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTCCATAACA TCAAACATCG ACCCACGGCG
     4141 TAACGCGCTT GCTGCTTGGA TGCCCGAGGC ATAGACTGTA CCCCAAAAAA ACATGTCATA
     4201 ACAAGAAGCC ATGAAAACCG CCggatatat tccgcttcct cgctcactga ctcgctacgc
     4261 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     4321 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     4381 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     4441 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     4501 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     4561 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     4621 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     4681 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     4741 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     4801 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     4861 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     4921 agatcatctt attaatcaga taaaatattt gctcatgagc ccgaagtggc gagcccgatc
     4981 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     5041 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atcGAGCTC
//
