LOCUS       213-petDuet1/GBD-LUMP--cdc42    6596 bp      DNA     circular   5/11/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1624..1642
                     /label=T7 terminal-reverse
                     /SerialCloner_Color=&h34C08A
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    71..202
                     /label=GBD(ACK)->cdc42
                     /SerialCloner_Color=&hA610C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNG" \ 
     misc_feature    203..217
                     /label=Linker
                     /SerialCloner_Color=&hC07518
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="SGSAS" \ 
     misc_feature    218..772
                     /label=LUMP
                     /SerialCloner_Color=&h1EC0C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="FRGIVQGRGVIRSISKSEDSQRHGIAFPEGMFQLVDVDTVMLVNGCSLTVVRILGDMVYFDIDQALGTTTFDGLKEGDQVNLEIHPKFGEVVGRGGLTGNIKGTALVAAIEENDAGFSVLIDIPKGLAENLTVKDDIGIDGISLPITDMSDSIITLNYSRDLLASTNIASLAKDVKVNVEILNEW" \ 
     misc_feature    945..1517
                     /label=Cdc42
                     /SerialCloner_Color=&hC00624
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL" \ 
     primer_bind     859..878
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     6580..3
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      3304..3932
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(1727..2167)
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      1722..2177
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    3..25
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    878..900
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(2295..2954)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5110..6066)
                     /label=LacI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(3195..3223)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1542..1586
                     /label=S-tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 
       61 gagatataCC ATGGGCCTGA GCGCACAGGA CATCAGCCAG CCGCTGCAGA ACAGCTTCAT 
      121 CCATACCGGC CACGGCGACA GCGATCCTCG CCATTGCTGG GGCTTTCCGG ACCGCATCGA 
      181 CGAGCTGTAC CTGGGCAACG GCAGCGGCAG CGCTAGCTTT AGAGGTATTG TTCAAGGTCG 
      241 TGGTGTTATT CGATCCATTT CAAAAAGTGA AGATAGTCAG CGACATGGGA TTGCATTCCC 
      301 TGAGGGTATG TTTCAACTTG TTGATGTAGA TACTGTAATG TTAGTAAATG GTTGTTCATT 
      361 GACAGTTGTC CGTATTTTAG GCGATATGGT GTACTTTGAT ATCGACCAAG CATTGGGTAC 
      421 GACAACATTT GATGGATTAA AAGAAGGCGA TCAGGTAAAC CTAGAGATCC ACCCTAAATT 
      481 TGGTGAAGTG GTTGGCCGTG GTGGATTAAC AGGAAATATT AAAGGCACTG CGCTGGTCGC 
      541 AGCAATAGAA GAGAATGACG CAGGTTTTTC TGTACTGATT GATATCCCTA AAGGGTTAGC 
      601 TGAAAACTTA ACAGTGAAAG ATGATATTGG AATTGATGGG ATCTCTTTGC CTATCACTGA 
      661 TATGTCAGAC AGCATTATTA CATTGAATTA CTCGCGTGAT TTGTTAGCGA GTACTAACAT 
      721 TGCATCGTTA GCAAAAGATG TGAAAGTGAA CGTTGAAATT TTAAATGAAT GGTAGGCGGC 
      781 CGCACTCAAG CTTgcggccg cataatgctt aagtcgaaca gaaagtaatc gtattgtaca 
      841 cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt 
      901 ccccatctta gtatattagt taagtataag aaggagatat aCATATGCAG ACAATTAAGT 
      961 GTGTTGTTGT GGGCGATGGT GCTGTTGGTA AAACATGTCT CCTGATATCC TACACAACAA 
     1021 ACAAATTTCC ATCGGAATAT GTACCGACTG TTTTTGACAA CTATGCAGTC ACAGTTATGA 
     1081 TTGGTGGAGA ACCATATACT CTTGGACTTT TTGATACTGC AGGGCAAGAG GATTATGATA 
     1141 GATTACGACC GCTGAGTTAT CCACAAACAG ATGTATTTCT AGTCTGTTTT TCAGTGGTCT 
     1201 CTCCATCTTC ATTTGAAAAC GTGAAAGAAA AGTGGGTGCC TGAGATAACT CACCACTGTC 
     1261 CAAAGACTCC TTTCTTGCTT GTTGGGACTC AAATTGATCT CAGAGATGAC CCCTCTACTA 
     1321 TTGAGAAACT TGCCAAGAAC AAACAGAAGC CTATCACTCC AGAGACTGCT GAAAAGCTGG 
     1381 CCCGTGACCT GAAGGCTGTC AAGTATGTGG AGTGTTCTGC ACTTACACAG AAAGGCCTAA 
     1441 AGAATGTATT TGACGAAGCA ATATTGGCTG CCCTGGAGCC TCCAGAACCG AAGAAGAGCC 
     1501 GCAGGTGTGT GCTGCTATGA GCGGCCGCAC TCGAGtctgg taaagaaacc gctgctgcga 
     1561 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg 
     1621 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 
     1681 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc 
     1741 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     1801 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     1861 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     1921 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     1981 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     2041 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     2101 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     2161 attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag gatcttcacc 
     2221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     2281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     2341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     2401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     2461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     2521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     2581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     2641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     2701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     2761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     2821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     2881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     2941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     3001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     3061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     3121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc atgattgaag 
     3181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     3241 acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     3301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     3361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     3421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 
     3481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     3541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     3601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     3661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     3721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     3781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     3841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     3901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     3961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     4021 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 
     4081 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
     4141 cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 
     4201 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 
     4261 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 
     4321 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 
     4381 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca 
     4441 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg 
     4501 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt 
     4561 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact 
     4621 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg 
     4681 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta 
     4741 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag 
     4801 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt 
     4861 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt 
     4921 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg 
     4981 agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct 
     5041 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg 
     5101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5161 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac 
     5221 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa 
     5281 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg 
     5341 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac 
     5401 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac 
     5461 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga 
     5521 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata 
     5581 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag 
     5641 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc 
     5701 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg 
     5761 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat 
     5821 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac 
     5881 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt 
     5941 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat 
     6001 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc 
     6061 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac 
     6121 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac 
     6181 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg 
     6241 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct 
     6301 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg 
     6361 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca 
     6421 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt 
     6481 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc 
     6541 cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actata
//