LOCUS       H14\pZDest\DF\Rosa26\Left\Gateway\CAGGS\LSLneo\rtTA\Luc\TdTomato       15320 bp    DNA     circular SYN 24-OCT-2014
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
COMMENT     ORIGDB|GenBank
            VNTDATE|596903900|
            VNTDBDATE|617392630|
            LSOWNER|
            VNTNAME|murRosa26_pZDonor|
            VNTAUTHORNAME|David Briner|
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|712687186|
COMMENT     LSOWNER|
COMMENT     VNTNAME|H14 pZDest DF Rosa26 Left Gateway CAGGS LSLneo rtTA Luc TdTomato|
COMMENT     VNTAUTHORNAME|mit|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                           "Required for secretion to the periplasm cleaved off to form the mature beta-lactamase protein." 
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COMMENT                            1 5 "ACCGGT" "TGGCCA") 
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FEATURES             Location/Qualifiers
     promoter        complement(109..137)
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="AmpR promoter"
     sig_peptide     179..247
                     /gene="bla"
                     /vntifkey="94"
                     /label=bla
                     /note="Required for secretion to the periplasm cleaved off to form the mature beta-lactamase protein."
     CDS             179..1039
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /vntifkey="4"
                     /label=bla\&\AmpR
                     /note="E-286"
     primer_bind     complement(205..225)
                     /vntifkey="28"
                     /label=Primer_Bind_1
                     /note="PCR LoxP F"
     primer_bind     complement(478..500)
                     /vntifkey="28"
                     /label=Primer_Bind_2
                     /note="EuMyc-2"
     rep_origin      1105..1803
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)"
     rep_origin      complement(1134..1816)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="ColE1-derived plasmid replication origin"
     repeat_region   complement(1147..1184)
                     /rpt_type=inverted
                     /vntifkey="34"
                     /label=Repeat_Region_1
                     /note="corresponds to one of the 38bp repeats found in Tn3 (bp 1-38 and complement (4920-4957))"
     conflict        1184..1857
                     /vntifkey="83"
                     /label=Conflict_1
                     /note="pUC origin"
     misc_feature    complement(1224..1859)
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="ori"
     misc_feature    complement(2156..2178)
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="Lac O"
     primer_bind     complement(2165..2187)
                     /vntifkey="28"
                     /label=Primer_Bind_3
                     /note="M13 pUC rev Primer"
     primer_bind     complement(2184..2204)
                     /vntifkey="28"
                     /label=Primer_Bind_4
                     /note="M13 Reverse"
     misc_recomb     2278..3075
                     /vntifkey="86"
                     /label=left\arm
                     /note="Left Homology Arm"
     primer_bind     2874..2894
                     /vntifkey="28"
                     /label=Primer_Bind_5
                     /note="Cre#1/LacZ#3"
     misc_feature    <3091..3106
                     /vntifkey="21"
                     /label=Misc_Feature_5
                     /note="Putative Primer Binding Area"
     misc_recomb     3107..3226
                     /vntifkey="86"
                     /label=attR1
                     /note="attR1"
     gene            3340..3999
                     /vntifkey="60"
                     /note="CMr"
     primer_bind     complement(3433..3452)
                     /vntifkey="28"
                     /label=Primer_Bind_6
                     /note="CMr_Seq(R)"
     gene            4341..4646
                     /vntifkey="60"
                     /note="ccdB"
     primer_bind     complement(4483..4502)
                     /vntifkey="28"
                     /label=Primer_Bind_7
                     /note="ccdBSeq(R)"
     misc_recomb     4687..4787
                     /vntifkey="86"
                     /label=attR2
                     /note="attR2"
     primer_bind     4810..4829
                     /vntifkey="28"
                     /label=Primer_Bind_8
                     /note="RosaDESTScrnR"
     enhancer        4868..5231
                     /vntifkey="9"
                     /label=CMV\immediate\early\region
                     /note="CMV immediate early region"
     primer_bind     5045..5067
                     /vntifkey="28"
                     /label=Primer_Bind_9
                     /note="caggsF1"
     promoter        5232..5513
                     /vntifkey="30"
                     /label=CAGGS
                     /note="CAGGS"
     primer_bind     complement(5250..5271)
                     /vntifkey="28"
                     /label=Primer_Bind_10
                     /note="caggsR1"
     primer_bind     5252..5274
                     /vntifkey="28"
                     /label=Primer_Bind_11
                     /note="caggsF2"
     primer_bind     complement(5449..5469)
                     /vntifkey="28"
                     /label=Primer_Bind_12
                     /note="caggsR2"
     intron          5514..6486
                     /vntifkey="15"
                     /label=Intron_1
                     /note="Intron_1; chicken beta-actin"
     intron          6487..6531
                     /vntifkey="15"
                     /label=Intron_2
                     /note="Intron_2; rabbit beta-globin"
     CDS             6622..6654
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     promoter        6675..7192
                     /vntifkey="30"
                     /label=PGK
                     /note="PGK"
     primer_bind     7148..7171
                     /vntifkey="28"
                     /label=Primer_Bind_13
                     /note="Reverse Primer"
     CDS             7194..7994
                     /vntifkey="4"
                     /label=NEO
                     /note="NEO"
     primer_bind     8402..8425
                     /vntifkey="28"
                     /label=Primer_Bind_14
                     /note="PCR for LoxP site"
     terminator      complement(8525..8746)
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="sv40 late pA"
     terminator      complement(8779..9000)
                     /vntifkey="43"
                     /label=Terminator_2
                     /note="sv40 late pA"
     terminator      complement(9033..9254)
                     /vntifkey="43"
                     /label=Terminator_3
                     /note="sv40 late pA"
     CDS             9288..9320
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     primer_bind     9351..9371
                     /vntifkey="28"
                     /label=Primer_Bind_15
                     /note="Kozak_rtTA3(F)"
     gene            9356..>10099
                     /vntifkey="60"
                     /note="rtTA3"
     primer_bind     complement(10148..10168)
                     /vntifkey="28"
                     /label=Primer_Bind_16
                     /note="T2a(r)"
     gene            10175..11602
                     /vntifkey="60"
                     /note="TdTomato"
     J_segment       <11618..11677
                     /vntifkey="16"
                     /label=2A
                     /note="E2a"
     CDS             11684..13513
                     /vntifkey="4"
                     /label=Luc2
                     /note="Luc2"
     primer_bind     13244..13263
                     /vntifkey="28"
                     /label=Primer_Bind_17
                     /note="Luc2-Seq"
     misc_signal     13546..13767
                     /vntifkey="87"
                     /label=sv40\late\pA
                     /note="SV40 late poly(A) signal"
     primer_bind     complement(13765..13791)
                     /vntifkey="28"
                     /label=Primer_Bind_18
                     /note="SV-Reverse (Phos)"
     misc_recomb     complement(13800..13833)
                     /vntifkey="86"
                     /label=Frt
                     /note="Frt"
     misc_recomb     13901..14712
                     /vntifkey="86"
                     /label=Right\Homology\Arm
                     /note="Right Homology Arm"
     primer_bind     complement(13922..13944)
                     /vntifkey="28"
                     /label=Primer_Bind_19
                     /note="CreER#2"
     primer_bind     complement(14275..14295)
                     /vntifkey="28"
                     /label=Primer_Bind_20
                     /note="LacZ#1"
     primer_bind     14839..14860
                     /vntifkey="28"
                     /label=Primer_Bind_21
                     /note="EuMyc-1"
     misc_feature    complement(14843..14911)
                     /vntifkey="21"
                     /label=Misc_Feature_12
                     /note="LacZ alpha"
     mat_peptide     14930..15236
                     /vntifkey="84"
                     /label=Mat_Peptide_1
                     /note="f1 Ori"
     CDS             9356..10099
                     /vntifkey="4"
                     /label=rtTA
     CDS             10175..11602
                     /vntifkey="4"
                     /label=TdTomato
     misc_feature    10115..10168
                     /vntifkey="21"
                     /label=2A
     CDS             4341..4646
                     /vntifkey="4"
                     /label=ccdb
     misc_feature    6622..9320
                     /vntifkey="21"
                     /label=LSL
     CDS             6543..6562
                     /vntifkey="4"
                     /label=pZ-F
     CDS             9462..9480
                     /vntifkey="4"
                     /label=pZ-rtTA-R
     primer          9351..9371
                     /vntifkey="27"
                     /label=pZ\15F
     primer          complement(4810..4829)
                     /vntifkey="27"
                     /label=pZ\8R
     misc_feature    6539..6558
                     /vntifkey="21"
                     /label=CAG-F
     primer          complement(10197..10216)
                     /vntifkey="27"
                     /label=14\TdTomato\Reverse
BASE COUNT     3502 a      4060 c      4192 g      3566 t 
ORIGIN
        1 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 
       61 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
      121 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
      181 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
      241 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
      301 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
      361 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
      421 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
      481 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
      541 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
      601 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
      661 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
      721 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
      781 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
      841 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
      901 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
      961 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     1021 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     1081 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     1141 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     1201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     1261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     1321 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 
     1381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     1441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     1501 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     1561 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     1621 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     1681 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     1741 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     1801 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 
     1861 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
     1921 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
     1981 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     2041 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
     2101 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
     2161 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 
     2221 aattaaccct cactaaaggg aacaaaagct ggagctctgt tttggttggc gtaaggcgcc 
     2281 tgtcagttaa cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg 
     2341 gggcgggagg taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg 
     2401 ggcgggggag gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct 
     2461 ccccttcctc tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc 
     2521 tctcggggcc cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat 
     2581 ccgccggcca gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc 
     2641 gcgccgcaga gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc 
     2701 ggggacgggc agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag 
     2761 ttgcctcaag aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga 
     2821 aggagcgagg gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg 
     2881 ctctgagttg ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct 
     2941 tctccggagg ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg 
     3001 gcttctgagg accgccctgg gcctgggaga atcccttccc cctcttccct cgtgatctgc 
     3061 aactccagtc tttctcccgg gagagtcgag gaattctgca gatatcacaa gtttgtacaa 
     3121 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 
     3181 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca 
     3241 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt 
     3301 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 
     3361 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 
     3421 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 
     3481 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 
     3541 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 
     3601 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 
     3661 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 
     3721 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 
     3781 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 
     3841 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 
     3901 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa 
     3961 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta 
     4021 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact 
     4081 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga 
     4141 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct 
     4201 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 
     4261 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 
     4321 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt 
     4381 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 
     4441 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 
     4501 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 
     4561 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 
     4621 ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc cagtctgcag 
     4681 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 
     4741 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg 
     4801 tacaaagtgg tgataaaccc gctgatcagc tcgacgttta aacctcgaga ttcgacattg 
     4861 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 
     4921 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 
     4981 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 
     5041 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 
     5101 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 
     5161 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 
     5221 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 
     5281 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 
     5341 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 
     5401 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 
     5461 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cggggagtcg 
     5521 ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg 
     5581 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc 
     5641 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt 
     5701 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt 
     5761 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg 
     5821 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt 
     5881 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 
     5941 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct 
     6001 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg 
     6061 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
     6121 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt 
     6181 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga 
     6241 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct 
     6301 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 
     6361 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg 
     6421 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc 
     6481 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc 
     6541 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattggccgc tctagaacta 
     6601 gtggatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc 
     6661 cctcgacctg caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 
     6721 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca 
     6781 cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 
     6841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 
     6901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 
     6961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 
     7021 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 
     7081 gggcgggcgc gaaggtcctc ccgaggcccg gcattctcgc acgcttcaaa agcgcacgtc 
     7141 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aatatgggat 
     7201 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 
     7261 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 
     7321 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 
     7381 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 
     7441 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 
     7501 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 
     7561 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 
     7621 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 
     7681 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 
     7741 acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 
     7801 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 
     7861 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 
     7921 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 
     7981 ttgacgagtt cttctgaggg gatccgctgt aagtctgcag aaattgatga tctattaaac 
     8041 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga 
     8101 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga 
     8161 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca 
     8221 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca 
     8281 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac 
     8341 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga 
     8401 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc 
     8461 catcagaagc ttgcagatct gcgactctag aggatctgcg actctagagg atcataatca 
     8521 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 
     8581 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 
     8641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     8701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta 
     8761 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 
     8821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 
     8881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     8941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     9001 gatctgcgac tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct 
     9061 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 
     9121 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     9181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     9241 tcttatcatg tctggatccc catcaagctg atccggaacc cttaatataa cttcgtataa 
     9301 tgtatgctat acgaagttat taggtccctc gacctgcagc ccaagctagg ccaccatgtc 
     9361 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat 
     9421 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta 
     9481 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag 
     9541 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa 
     9601 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg 
     9661 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca 
     9721 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact 
     9781 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac 
     9841 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc 
     9901 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa 
     9961 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc 
    10021 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga 
    10081 ccttgacatg ctccccgggg gaagtggagc tagggagggc agaggaagtc ttctaacatg 
    10141 cggtgacgtg gaggagaatc ccggccctgc tagaatggtg agcaagggcg aggaggtcat 
    10201 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga 
    10261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 
    10321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg 
    10381 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc 
    10441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac 
    10501 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa 
    10561 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga 
    10621 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa 
    10681 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca 
    10741 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta 
    10801 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg 
    10861 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc 
    10921 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga 
    10981 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 
    11041 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat 
    11101 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc 
    11161 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac 
    11221 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg 
    11281 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 
    11341 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa 
    11401 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc 
    11461 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga 
    11521 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta 
    11581 cggcatggac gagctgtaca agggaagtgg atctagccag tgtactaatt atgctctctt 
    11641 gaaattggct ggagatgttg agagcaaccc aggtccctct aggatggaag atgccaaaaa 
    11701 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct 
    11761 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca 
    11821 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc 
    11881 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt 
    11941 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa 
    12001 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt 
    12061 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat 
    12121 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac 
    12181 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt 
    12241 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa 
    12301 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat 
    12361 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg 
    12421 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta 
    12481 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct 
    12541 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct 
    12601 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc 
    12661 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac 
    12721 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt 
    12781 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca 
    12841 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga 
    12901 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg 
    12961 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg 
    13021 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga 
    13081 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt 
    13141 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt 
    13201 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact 
    13261 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg 
    13321 caagatcgcc gtgaattctg cttgcaagaa ctggttcagt agcttaagcc actttgtgat 
    13381 ccaccttaac agccacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc 
    13441 catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag 
    13501 gatcaacgtc taacctagaa ctagcttatc gatggccgct tcgagcagac atgataagat 
    13561 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 
    13621 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 
    13681 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 
    13741 gcaagtaaaa cctctacaaa tgtggtaaaa tcggatccac tagttctaga gcggccatcg 
    13801 aagttcctat actttctaga gaataggaac ttcggaatag taacttctcc ataagcttca 
    13861 gctgctcgag accggtggat ccgaattcag accgcggaga tgggcgggag tcttctgggc 
    13921 aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa 
    13981 aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg 
    14041 gcaaataagg aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag 
    14101 gttattattg cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc 
    14161 tcacccgagt tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc 
    14221 gcgagttaga ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc 
    14281 catttctccc gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc 
    14341 ccattttcat ttattatact ggcttatcca acccctagac agagcattgg cattttccct 
    14401 ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca 
    14461 aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt 
    14521 tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg 
    14581 gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag 
    14641 aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt 
    14701 ataaactgag ctggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 
    14761 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 
    14821 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 
    14881 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 
    14941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
    15001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
    15061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
    15121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
    15181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
    15241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
    15301 gttaaaaaat gagctgattt 
//
