LOCUS       p189_pCCL-PGK-SPdCas9-BFP-DNMT3B     13037 bp    DNA     circular UNA 19-DEC-2012
DEFINITION  
ACCESSION   pCCL-PGK-eGFP
DBSOURCE    Private
COMMENT     This file is created by VectorDesigner
FEATURES             Location/Qualifiers
     misc_feature    108..679
                     /note="Oriinal location: 108..679"
                     /label="CMV Promoter"
     misc_feature    680..777
                     /note="Oriinal location: 680..777"
                     /label="R region of 5' LTR"
     misc_feature    778..860
                     /note="Oriinal location: 778..860"
                     /label="U5 region of 5' LTR"
     misc_feature    861..1011
                     /note="Oriinal location: 861..1011"
                     /label="psi packaging signal"
     misc_feature    1013..1015
                     /note="Oriinal location: 1013..1015"
                     /label="gag start codon"
     misc_feature    1076..1078
                     /note="Oriinal location: 1076..1078"
                     /label="gag stop codon"
     misc_feature    1375..1615
                     /note="Oriinal location: 1375..1615"
                     /label=RRE
     misc_feature    2101..2130
                     /note="Oriinal location: 2101..2130"
                     /label="Splice Acceptor"
     misc_feature    2167..2277
                     /note="Oriinal location: 2167..2277"
                     /label=cPPT
     misc_feature    2402..2890
                     /note="Oriinal location: 2402..2890"
                     /label="PGK Promoter"
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             2970..7076
                     /label=dCas9
     CDS             2970..7955
                     /label=dCas9-BFP-linker
     CDS             2970..8915
                     /label="fusion protein"
     Site            join(6458..6463,6460^6461)
                     /site_type="restriction site"
                     /note="Name: SmaI"
                     /note="Pattern: cccggg"
                     /note="cut_0_on_positive_strand: 2930^2931"
                     /note="cut_0_on_negative_strand: 2930^2931"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SmaI
     Site            join(6458..6463,6460^6461)
                     /site_type="restriction site"
                     /note="Name: SmaI"
                     /note="Pattern: cccggg"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 2930^2931"
                     /note="cut_0_on_negative_strand: 2930^2931"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SmaI
     misc_feature    7043..7062
                     /feature_type="DNA/RNA Features"
                     /label=Seq-1
     CDS             7077..7103
                     /label="HA tag"
     CDS             7104..7121
                     /label="GS linker"
     CDS             7122..7142
                     /label=NLS
     CDS             7149..7168
                     /label=NLS
     CDS             7169..7208
     Site            join(7203..7208,7203^7204)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 7203^7204"
                     /note="cut_0_on_negative_strand: 7207^7208"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(7203..7208,7203^7204)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 7203^7204"
                     /note="cut_0_on_negative_strand: 7207^7208"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             7209..7904
                     /label=BFP
     misc_feature    7726..7745
                     /feature_type="DNA/RNA Features"
                     /label=BFP-F
     CDS             7905..7949
                     /label=3XG4S
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     CDS             7956..8915
                     /label=DNMT3B
     Site            join(8613..8618,8613^8614)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 8613^8614"
                     /note="cut_0_on_negative_strand: 8617^8618"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(8919..8924,8923^8924)
                     /site_type="restriction site"
                     /note="Name: NsiI"
                     /note="Pattern: atgcat"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9400^9401"
                     /note="cut_0_on_negative_strand: 9396^9397"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NsiI
     Site            join(8919..8924,8923^8924)
                     /site_type="restriction site"
                     /note="Name: NsiI"
                     /note="Pattern: atgcat"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9400^9401"
                     /note="cut_0_on_negative_strand: 9396^9397"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NsiI
     Site            join(8925..8932,8930^8931)
                     /site_type="restriction site"
                     /note="Name: FseI"
                     /note="Pattern: ggccggcc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9407^9408"
                     /note="cut_0_on_negative_strand: 9403^9404"
                     /note="inhibited_by: 5': 5-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=FseI
     Site            join(8933..8940,8937^8938)
                     /site_type="restriction site"
                     /note="Name: PacI"
                     /note="Pattern: ttaattaa"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9414^9415"
                     /note="cut_0_on_negative_strand: 9412^9413"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=PacI
     Site            join(8941..8946,8941^8942)
                     /site_type="restriction site"
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 3679^3680"
                     /note="cut_0_on_negative_strand: 3683^3684"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Site            join(8941..8946,8941^8942)
                     /site_type="restriction site"
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 3679^3680"
                     /note="cut_0_on_negative_strand: 3683^3684"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Site            join(8947..8952,8947^8948)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 3685^3686"
                     /note="cut_0_on_negative_strand: 3689^3690"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(8947..8952,8947^8948)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 8947^8948"
                     /note="cut_0_on_negative_strand: 8951^8952"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(8954..8959,8954^8955)
                     /site_type="restriction site"
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 3692^3693"
                     /note="cut_0_on_negative_strand: 3696^3697"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
     misc_feature    8963..9552
                     /note="Oriinal location: 3677..4266"
                     /label=WPRE
     misc_feature    complement(8999..9019)
                     /feature_type="DNA/RNA Features"
                     /label=Seq-2
     misc_feature    9641..9695
                     /note="Oriinal location: 4355..4409"
                     /label="U3 region of 3' LTR (SIN)"
     misc_feature    9696..9791
                     /note="Oriinal location: 4410..4505"
                     /label="R region of 3' LTR"
     misc_feature    9792..9874
                     /note="Oriinal location: 4506..4588"
                     /label="U5 region of 3' LTR"
ORIGIN
        1 AAGCTTGGCC ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT
       61 GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
      121 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
      181 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
      241 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
      301 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
      361 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
      421 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
      481 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
      541 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA
      601 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
      661 GAGCTCGTTT AGTGAACCGG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
      721 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
      781 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
      841 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACC TGAAAGCGAA AGGGAAACCA
      901 GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG
      961 CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG AGATGGGTGC
     1021 GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT TCGGTTAAGG
     1081 CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA
     1141 CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA
     1201 CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA
     1261 GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC
     1321 AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC CGCTGATCTT
     1381 CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA AATATAAAGT
     1441 AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG TGGTGCAGAG
     1501 AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG
     1561 CACTATGGGC GCAGCCTCAA TGACGCTGAC GGTACAGGCC AGACAATTAT TGTCTGGTAT
     1621 AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG CAACAGCATC TGTTGCAACT
     1681 CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG GCTGTGGAAA GATACCTAAA
     1741 GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT
     1801 GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG ATTGGAATCA CACGACCTGG
     1861 ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA
     1921 TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT
     1981 TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA
     2041 GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT
     2101 AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC
     2161 AGGCCCGAAG GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA
     2221 GTGAACGGAT CTCGACGGTA TCGGTTAACT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC
     2281 AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA
     2341 AAACAAATTA CAAAAATTCA AAATTTTATC GATCACGAGA CTAGCCTCGA CGATGGTCGA
     2401 GTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC
     2461 GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG
     2521 TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT
     2581 AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT
     2641 AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG
     2701 CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG
     2761 AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG
     2821 GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC
     2881 CTCTTCCTCA TCTCCGGGCC TTTCGACCTC TAGCGGGATC CGGcgcgtct gcaggatatc
     2941 aagcttgcgg taccgcgggc ccgGCCACCA TGGACAAGAA GTACAGCATC GGCCTGGCCA
     3001 TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA
     3061 AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGCGCCC
     3121 TGCTGTTCGA CAGCGGAGAA ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA
     3181 GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG
     3241 CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA
     3301 AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA
     3361 AGTACCCCAC CATCTACCAC CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC
     3421 TGCGGCTGAT CTATCTGGCC CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG
     3481 AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA
     3541 CCTACAACCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA
     3601 TCCTGTCTGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG
     3661 GCGAGAAGAA GAATGGCCTG TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA
     3721 ACTTCAAGAG CAACTTCGAC CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT
     3781 ACGACGACGA CCTGGACAAC CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC
     3841 TGGCCGCCAA GAACCTGTCC GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG
     3901 AGATCACCAA GGCCCCCCTG AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG
     3961 ACCTGACCCT GCTGAAAGCT CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT
     4021 TCTTCGACCA GAGCAAGAAC GGCTACGCCG GCTACATCGA TGGCGGAGCC AGCCAGGAAG
     4081 AGTTCTACAA GTTCATCAAG CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG
     4141 TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC
     4201 CCCACCAGAT CCACCTGGGA GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC
     4261 CATTCCTGAA GGACAACCGG GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT
     4321 ACGTGGGCCC TCTGGCCAGG GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG
     4381 AAACCATCAC CCCCTGGAAC TTCGAGGAAG TGGTGGACAA GGGCGCCAGC GCCCAGAGCT
     4441 TCATCGAGCG GATGACCAAC TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC
     4501 ACAGCCTGCT GTACGAGTAC TTCACCGTGT ACAACGAGCT GACCAAAGTG AAATACGTGA
     4561 CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA GCGGCGAGCA GAAAAAAGCC ATCGTGGACC
     4621 TGCTGTTCAA GACCAACCGG AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA
     4681 AAATCGAGTG CTTCGACTCC GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC
     4741 TGGGCACATA CCACGATCTG CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG
     4801 AAAACGAGGA CATTCTGGAA GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA
     4861 TGATCGAGGA ACGGCTGAAA ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC
     4921 TGAAGCGGCG GAGATACACC GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC
     4981 GGGACAAGCA GTCCGGCAAG ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA
     5041 GAAACTTCAT GCAGCTGATC CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG
     5101 CCCAGGTGTC CGGCCAGGGC GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC
     5161 CCGCCATTAA GAAGGGCATC CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA
     5221 TGGGCCGGCA CAAGCCCGAG AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC
     5281 AGAAGGGACA GAAGAACAGC CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC
     5341 TGGGCAGCCA GATCCTGAAA GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC
     5401 TGTACCTGTA CTACCTGCAG AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA
     5461 ACCGGCTGTC CGACTACGAT GTGGACGCTA TCGTGCCTCA GAGCTTTCTG AAGGACGACT
     5521 CCATCGATAA CAAAGTGCTG ACTCGGAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC
     5581 CCTCCGAAGA GGTCGTGAAG AAGATGAAGA ACTACTGGCG CCAGCTGCTG AATGCCAAGC
     5641 TGATTACCCA GAGGAAGTTC GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC
     5701 TGGATAAGGC CGGCTTCATC AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG
     5761 TGGCACAGAT CCTGGACTCC CGGATGAACA CTAAGTACGA CGAGAACGAC AAACTGATCC
     5821 GGGAAGTGAA AGTGATCACC CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC
     5881 AGTTTTACAA AGTGCGCGAG ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG
     5941 CCGTCGTGGG AACCGCCCTG ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG
     6001 GCGACTACAA GGTGTACGAC GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA
     6061 AGGCTACCGC CAAGTACTTC TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA
     6121 CCCTGGCCAA CGGCGAGATC CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACAGGCG
     6181 AGATCGTGTG GGATAAGGGC CGGGACTTTG CCACCGTGCG GAAAGTGCTG TCTATGCCCC
     6241 AAGTGAATAT CGTGAAAAAG ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC
     6301 TGCCCAAGAG GAACAGCGAC AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT
     6361 ACGGCGGCTT CGACAGCCCC ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA
     6421 AGGGCAAGTC CAAGAAACTG AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA
     6481 GAAGCAGCTT CGAGAAGAAT CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA
     6541 AAAAGGACCT GATCATCAAG CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA
     6601 AGAGAATGCT GGCCTCTGCC GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA
     6661 AATATGTGAA CTTCCTGTAC CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG
     6721 ATAATGAGCA GAAACAGCTG TTTGTGGAAC AGCACAAACA CTACCTGGAC GAGATCATCG
     6781 AGCAGATCAG CGAGTTCTCC AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAGGTGC
     6841 TGAGCGCCTA CAACAAGCAC AGAGACAAGC CTATCAGAGA GCAGGCCGAG AATATCATCC
     6901 ACCTGTTTAC CCTGACCAAT CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA
     6961 TCGACCGGAA GAGGTACACC AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA
     7021 GCATCACCGG CCTGTACGAG ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACGCCTATC
     7081 CCTATGACGT GCCCGATTAT GCCAGCCTGG GCAGCGGCTC CCCCAAGAAA AAACGCAAGG
     7141 TGGAAGATCC TAAGAAAAAG CGGAAAGTGG ACGGCATTGG TAGTGGGAGC AACGGCAGCA
     7201 GCGGATCCAG CGAGCTGATT AAGGAGAACA TGCACATGAA GCTGTACATG GAGGGCACCG
     7261 TGGACAACCA TCACTTCAAG TGCACATCCG AGGGCGAAGG CAAGCCCTAC GAGGGCACCC
     7321 AGACCATGAG AATCAAGGTG GTCGAGGGCG GCCCTCTCCC CTTCGCCTTC GACATCCTGG
     7381 CTACTAGCTT CCTCTACGGC AGCAAGACCT TCATCAACCA CACCCAGGGC ATCCCCGACT
     7441 TCTTCAAGCA GTCCTTCCCT GAGGGCTTCA CATGGGAGAG AGTCACCACA TACGAAGACG
     7501 GGGGCGTGCT GACCGCTACC CAGGACACCA GCCTCCAGGA CGGCTGCCTC ATCTACAACG
     7561 TCAAGATCAG AGGGGTGAAC TTCACATCCA ACGGCCCTGT GATGCAGAAG AAAACACTCG
     7621 GCTGGGAGGC CTTCACCGAG ACGCTGTACC CCGCTGACGG CGGCCTGGAA GGCAGAAACG
     7681 ACATGGCCCT GAAGCTCGTG GGCGGGAGCC ATCTGATCGC AAACATCAAG ACCACATATA
     7741 GATCCAAGAA ACCCGCTAAG AACCTCAAGA TGCCTGGCGT CTACTATGTG GACTACAGAC
     7801 TGGAAAGAAT CAAGGAGGCC AACAACGAGA CCTACGTCGA GCAGCACGAG GTGGCAGTGG
     7861 CCAGATACTG CGACCTCCCT AGCAAACTGG GGCACAAGCT TAATggtgga ggaggttctg
     7921 gaggcggtgg aagtggtggc ggaggtagcG TCGACGTCCT GCGGCGCCGG AAGGACTGGA
     7981 ACGTGCGCCT GCAGGCCTTC TTCACCAGTG ACACGGGGCT TGAATAtGAA GCCCCCAAGC
     8041 TGTACCCTGC CATTCCCGCA GCCCGAAGGC GGCCCATTCG AGTCCTGTCA TTGTTTGATG
     8101 GCATCGCGAC AGGCTACCTA GTCCTCAAAG AGTTGGGCAT AAAGGTAGGA AAGTACGTCG
     8161 CTTCTGAAGT GTGTGAGGAG TCCATTGCTG TTGGAACCGT GAAGCACGAG GGGAATATCA
     8221 AATACGTGAA CGACGTGAGG AACATCACAA AGAAAAATAT TGAAGAATGG GGCCCATTTG
     8281 ACTTGGTGAT TGGCGGAAGC CCATGCAACG ATCTCTCAAA TGTGAATCCA GCCAGGAAAG
     8341 GCCTGTATGA GGGTACAGGC CGGCTCTTCT TCGAATTTTA CCACCTGCTG AATTACTCAC
     8401 GCCCCAAGGA GGGTGATGAC CGGCCGTTCT TCTGGATGTT TGAGAATGTT GTAGCCATGA
     8461 AGGTTGGCGA CAAGAGGGAC ATCTCACGGT TCCTGGAGTG TAATCCAGTG ATGATTGATG
     8521 CCATCAAAGT TTCTGCTGCT CACAGGGCCC GATACTTCTG GGGCAACCTA CCCGGGATGA
     8581 ACAGGCCCGT GATAGCATCA AAGAATGATA AACTCGAGCT GCAGGACTGC TTGGAATACA
     8641 ATAGGATAGC CAAGTTAAAG AAAGTACAGA CAATAACCAC CAAGTCGAAC TCGATCAAAC
     8701 AGGGGAAAAA CCAACTTTTC CCTGTTGTCA TGAATGGCAA AGAAGATGTT TTGTGGTGCA
     8761 CTGAGCTCGA AAGGATCTTT GGCTTTCCTG TGCACTACAC AGACGTGTCC AACATGGGCC
     8821 GTGGTGCCCG CCAGAAGCTG CTGGGAAGGT CCTGGAGCGT GCCTGTCATC CGACACCTCT
     8881 TCGCCCCTCT GAAGGACTAC TTTGCATGTG AATAGTAAAT GCATGGCCGG CCTTAATTAA
     8941 ACGCGTCTCG AGGGAATTCC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA
     9001 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT
     9061 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT
     9121 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG
     9181 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG
     9241 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC
     9301 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC
     9361 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT
     9421 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG
     9481 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG
     9541 CCGCCTCCCC GCATCGGGAA TTCGAGCTCG GTACCTTTAA GACCAATGAC TTACAAGGCA
     9601 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC
     9661 CAACGAAGAC AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA
     9721 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT
     9781 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC
     9841 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATCT AGCTAGAATT AATTCCGTGT
     9901 ATTCTATAGT GTCACCTAAA TCGTATGTGT ATGATACATA AGGTTATGTA TTAATTGTAG
     9961 CCGCGTTCTA ACGACAATAT GTACAAGCCT AATTGTGTAG CATCTGGCTT ACTGAAGCAG
    10021 ACCCTATCAT CTCTCTCGTA AACTGCCGTC AGAGTCGGTT TGGTTGGACG AACCTTCTGA
    10081 GTTTCTGGTA ACGCCGTCCC GCACCCGGAA ATGGTCAGCG AACCAATCAG CAGGGTCATC
    10141 GCTAGCCTAG GCTTTTGCGT CGAGACGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG
    10201 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
    10261 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
    10321 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCGAC GCGCCCTGTA
    10381 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA
    10441 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT
    10501 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC
    10561 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
    10621 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC
    10681 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC
    10741 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA
    10801 ACAAAATATT AACGTTTACA ATTTCCCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
    10861 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
    10921 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
    10981 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
    11041 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
    11101 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
    11161 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
    11221 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
    11281 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
    11341 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
    11401 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
    11461 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
    11521 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
    11581 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
    11641 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
    11701 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
    11761 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
    11821 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
    11881 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
    11941 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
    12001 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
    12061 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
    12121 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
    12181 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
    12241 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
    12301 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
    12361 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
    12421 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
    12481 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
    12541 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
    12601 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
    12661 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
    12721 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC
    12781 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA
    12841 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC
    12901 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
    12961 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA
    13021 ACAAAAGCTG GAGCTGC
//
