LOCUS       p191_pCCL-PGK-SPdCas9-BFP-EGFP     12791 bp    DNA     circular UNA 19-DEC-2012
DEFINITION  
ACCESSION   pCCL-PGK-eGFP
DBSOURCE    Private
COMMENT     This file is created by VectorDesigner
FEATURES             Location/Qualifiers
     misc_feature    108..679
                     /note="Oriinal location: 108..679"
                     /label="CMV Promoter"
     misc_feature    680..777
                     /note="Oriinal location: 680..777"
                     /label="R region of 5' LTR"
     misc_feature    778..860
                     /note="Oriinal location: 778..860"
                     /label="U5 region of 5' LTR"
     misc_feature    861..1011
                     /note="Oriinal location: 861..1011"
                     /label="psi packaging signal"
     misc_feature    1013..1015
                     /note="Oriinal location: 1013..1015"
                     /label="gag start codon"
     misc_feature    1076..1078
                     /note="Oriinal location: 1076..1078"
                     /label="gag stop codon"
     misc_feature    1375..1615
                     /note="Oriinal location: 1375..1615"
                     /label=RRE
     misc_feature    2101..2130
                     /note="Oriinal location: 2101..2130"
                     /label="Splice Acceptor"
     misc_feature    2167..2277
                     /note="Oriinal location: 2167..2277"
                     /label=cPPT
     misc_feature    2402..2890
                     /note="Oriinal location: 2402..2890"
                     /label="PGK Promoter"
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(2916..2921,2916^2917)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 2916^2917"
                     /note="cut_0_on_negative_strand: 2920^2921"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             2970..7076
                     /label=dCas9
     CDS             2970..7955
                     /label=dCas9-BFP-linker
     CDS             2970..8666
                     /label="Fusion protein"
     Site            join(6458..6463,6460^6461)
                     /site_type="restriction site"
                     /note="Name: SmaI"
                     /note="Pattern: cccggg"
                     /note="cut_0_on_positive_strand: 2930^2931"
                     /note="cut_0_on_negative_strand: 2930^2931"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SmaI
     Site            join(6458..6463,6460^6461)
                     /site_type="restriction site"
                     /note="Name: SmaI"
                     /note="Pattern: cccggg"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 2930^2931"
                     /note="cut_0_on_negative_strand: 2930^2931"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SmaI
     misc_feature    7043..7062
                     /feature_type="DNA/RNA Features"
                     /label=Seq-1
     CDS             7077..7103
                     /label="HA tag"
     CDS             7104..7121
                     /label="GS linker"
     CDS             7122..7142
                     /label=NLS
     CDS             7149..7168
                     /label=NLS
     CDS             7169..7208
     Site            join(7203..7208,7203^7204)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 7203^7204"
                     /note="cut_0_on_negative_strand: 7207^7208"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(7203..7208,7203^7204)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 7203^7204"
                     /note="cut_0_on_negative_strand: 7207^7208"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     CDS             7209..7904
                     /label=BFP
     misc_feature    7726..7745
                     /feature_type="DNA/RNA Features"
                     /label=BFP-F
     CDS             7905..7949
                     /label=3XG4S
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(7950..7955,7950^7951)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7950^7951"
                     /note="cut_0_on_negative_strand: 7954^7955"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     CDS             7956..8669
                     /label=EGFP
     Site            join(8673..8678,8677^8678)
                     /site_type="restriction site"
                     /note="Name: NsiI"
                     /note="Pattern: atgcat"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9400^9401"
                     /note="cut_0_on_negative_strand: 9396^9397"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NsiI
     Site            join(8673..8678,8677^8678)
                     /site_type="restriction site"
                     /note="Name: NsiI"
                     /note="Pattern: atgcat"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9400^9401"
                     /note="cut_0_on_negative_strand: 9396^9397"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NsiI
     Site            join(8679..8686,8684^8685)
                     /site_type="restriction site"
                     /note="Name: FseI"
                     /note="Pattern: ggccggcc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9407^9408"
                     /note="cut_0_on_negative_strand: 9403^9404"
                     /note="inhibited_by: 5': 5-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=FseI
     Site            join(8687..8694,8691^8692)
                     /site_type="restriction site"
                     /note="Name: PacI"
                     /note="Pattern: ttaattaa"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 9414^9415"
                     /note="cut_0_on_negative_strand: 9412^9413"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=PacI
     Site            join(8695..8700,8695^8696)
                     /site_type="restriction site"
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="cut_0_on_positive_strand: 3679^3680"
                     /note="cut_0_on_negative_strand: 3683^3684"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Site            join(8695..8700,8695^8696)
                     /site_type="restriction site"
                     /note="Name: MluI"
                     /note="Pattern: acgcgt"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 3679^3680"
                     /note="cut_0_on_negative_strand: 3683^3684"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=MluI
     Site            join(8701..8706,8701^8702)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 3685^3686"
                     /note="cut_0_on_negative_strand: 3689^3690"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(8701..8706,8701^8702)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 8701^8702"
                     /note="cut_0_on_negative_strand: 8705^8706"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(8708..8713,8708^8709)
                     /site_type="restriction site"
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 3692^3693"
                     /note="cut_0_on_negative_strand: 3696^3697"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
     misc_feature    8717..9306
                     /note="Oriinal location: 3677..4266"
                     /label=WPRE
     misc_feature    complement(8753..8773)
                     /feature_type="DNA/RNA Features"
                     /label=Seq-2
     misc_feature    9395..9449
                     /note="Oriinal location: 4355..4409"
                     /label="U3 region of 3' LTR (SIN)"
     misc_feature    9450..9545
                     /note="Oriinal location: 4410..4505"
                     /label="R region of 3' LTR"
     misc_feature    9546..9628
                     /note="Oriinal location: 4506..4588"
                     /label="U5 region of 3' LTR"
ORIGIN
        1 AAGCTTGGCC ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT
       61 GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
      121 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
      181 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
      241 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
      301 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
      361 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
      421 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
      481 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
      541 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA
      601 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
      661 GAGCTCGTTT AGTGAACCGG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
      721 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
      781 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
      841 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACC TGAAAGCGAA AGGGAAACCA
      901 GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG
      961 CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG AGATGGGTGC
     1021 GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT TCGGTTAAGG
     1081 CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA
     1141 CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA
     1201 CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA
     1261 GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC
     1321 AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC CGCTGATCTT
     1381 CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA AATATAAAGT
     1441 AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG TGGTGCAGAG
     1501 AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG
     1561 CACTATGGGC GCAGCCTCAA TGACGCTGAC GGTACAGGCC AGACAATTAT TGTCTGGTAT
     1621 AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG CAACAGCATC TGTTGCAACT
     1681 CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG GCTGTGGAAA GATACCTAAA
     1741 GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT
     1801 GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG ATTGGAATCA CACGACCTGG
     1861 ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA
     1921 TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT
     1981 TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA
     2041 GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT
     2101 AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC
     2161 AGGCCCGAAG GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA
     2221 GTGAACGGAT CTCGACGGTA TCGGTTAACT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC
     2281 AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA
     2341 AAACAAATTA CAAAAATTCA AAATTTTATC GATCACGAGA CTAGCCTCGA CGATGGTCGA
     2401 GTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC
     2461 GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG
     2521 TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT
     2581 AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT
     2641 AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG
     2701 CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG
     2761 AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG
     2821 GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC
     2881 CTCTTCCTCA TCTCCGGGCC TTTCGACCTC TAGCGGGATC CGGcgcgtct gcaggatatc
     2941 aagcttgcgg taccgcgggc ccgGCCACCA TGGACAAGAA GTACAGCATC GGCCTGGCCA
     3001 TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA
     3061 AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGCGCCC
     3121 TGCTGTTCGA CAGCGGAGAA ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA
     3181 GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG
     3241 CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA
     3301 AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA
     3361 AGTACCCCAC CATCTACCAC CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC
     3421 TGCGGCTGAT CTATCTGGCC CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG
     3481 AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA
     3541 CCTACAACCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA
     3601 TCCTGTCTGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG
     3661 GCGAGAAGAA GAATGGCCTG TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA
     3721 ACTTCAAGAG CAACTTCGAC CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT
     3781 ACGACGACGA CCTGGACAAC CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC
     3841 TGGCCGCCAA GAACCTGTCC GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG
     3901 AGATCACCAA GGCCCCCCTG AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG
     3961 ACCTGACCCT GCTGAAAGCT CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT
     4021 TCTTCGACCA GAGCAAGAAC GGCTACGCCG GCTACATCGA TGGCGGAGCC AGCCAGGAAG
     4081 AGTTCTACAA GTTCATCAAG CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG
     4141 TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC
     4201 CCCACCAGAT CCACCTGGGA GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC
     4261 CATTCCTGAA GGACAACCGG GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT
     4321 ACGTGGGCCC TCTGGCCAGG GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG
     4381 AAACCATCAC CCCCTGGAAC TTCGAGGAAG TGGTGGACAA GGGCGCCAGC GCCCAGAGCT
     4441 TCATCGAGCG GATGACCAAC TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC
     4501 ACAGCCTGCT GTACGAGTAC TTCACCGTGT ACAACGAGCT GACCAAAGTG AAATACGTGA
     4561 CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA GCGGCGAGCA GAAAAAAGCC ATCGTGGACC
     4621 TGCTGTTCAA GACCAACCGG AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA
     4681 AAATCGAGTG CTTCGACTCC GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC
     4741 TGGGCACATA CCACGATCTG CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG
     4801 AAAACGAGGA CATTCTGGAA GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA
     4861 TGATCGAGGA ACGGCTGAAA ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC
     4921 TGAAGCGGCG GAGATACACC GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC
     4981 GGGACAAGCA GTCCGGCAAG ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA
     5041 GAAACTTCAT GCAGCTGATC CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG
     5101 CCCAGGTGTC CGGCCAGGGC GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC
     5161 CCGCCATTAA GAAGGGCATC CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA
     5221 TGGGCCGGCA CAAGCCCGAG AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC
     5281 AGAAGGGACA GAAGAACAGC CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC
     5341 TGGGCAGCCA GATCCTGAAA GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC
     5401 TGTACCTGTA CTACCTGCAG AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA
     5461 ACCGGCTGTC CGACTACGAT GTGGACGCTA TCGTGCCTCA GAGCTTTCTG AAGGACGACT
     5521 CCATCGATAA CAAAGTGCTG ACTCGGAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC
     5581 CCTCCGAAGA GGTCGTGAAG AAGATGAAGA ACTACTGGCG CCAGCTGCTG AATGCCAAGC
     5641 TGATTACCCA GAGGAAGTTC GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC
     5701 TGGATAAGGC CGGCTTCATC AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG
     5761 TGGCACAGAT CCTGGACTCC CGGATGAACA CTAAGTACGA CGAGAACGAC AAACTGATCC
     5821 GGGAAGTGAA AGTGATCACC CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC
     5881 AGTTTTACAA AGTGCGCGAG ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG
     5941 CCGTCGTGGG AACCGCCCTG ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG
     6001 GCGACTACAA GGTGTACGAC GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA
     6061 AGGCTACCGC CAAGTACTTC TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA
     6121 CCCTGGCCAA CGGCGAGATC CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACAGGCG
     6181 AGATCGTGTG GGATAAGGGC CGGGACTTTG CCACCGTGCG GAAAGTGCTG TCTATGCCCC
     6241 AAGTGAATAT CGTGAAAAAG ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC
     6301 TGCCCAAGAG GAACAGCGAC AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT
     6361 ACGGCGGCTT CGACAGCCCC ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA
     6421 AGGGCAAGTC CAAGAAACTG AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA
     6481 GAAGCAGCTT CGAGAAGAAT CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA
     6541 AAAAGGACCT GATCATCAAG CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA
     6601 AGAGAATGCT GGCCTCTGCC GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA
     6661 AATATGTGAA CTTCCTGTAC CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG
     6721 ATAATGAGCA GAAACAGCTG TTTGTGGAAC AGCACAAACA CTACCTGGAC GAGATCATCG
     6781 AGCAGATCAG CGAGTTCTCC AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAGGTGC
     6841 TGAGCGCCTA CAACAAGCAC AGAGACAAGC CTATCAGAGA GCAGGCCGAG AATATCATCC
     6901 ACCTGTTTAC CCTGACCAAT CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA
     6961 TCGACCGGAA GAGGTACACC AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA
     7021 GCATCACCGG CCTGTACGAG ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACGCCTATC
     7081 CCTATGACGT GCCCGATTAT GCCAGCCTGG GCAGCGGCTC CCCCAAGAAA AAACGCAAGG
     7141 TGGAAGATCC TAAGAAAAAG CGGAAAGTGG ACGGCATTGG TAGTGGGAGC AACGGCAGCA
     7201 GCGGATCCAG CGAGCTGATT AAGGAGAACA TGCACATGAA GCTGTACATG GAGGGCACCG
     7261 TGGACAACCA TCACTTCAAG TGCACATCCG AGGGCGAAGG CAAGCCCTAC GAGGGCACCC
     7321 AGACCATGAG AATCAAGGTG GTCGAGGGCG GCCCTCTCCC CTTCGCCTTC GACATCCTGG
     7381 CTACTAGCTT CCTCTACGGC AGCAAGACCT TCATCAACCA CACCCAGGGC ATCCCCGACT
     7441 TCTTCAAGCA GTCCTTCCCT GAGGGCTTCA CATGGGAGAG AGTCACCACA TACGAAGACG
     7501 GGGGCGTGCT GACCGCTACC CAGGACACCA GCCTCCAGGA CGGCTGCCTC ATCTACAACG
     7561 TCAAGATCAG AGGGGTGAAC TTCACATCCA ACGGCCCTGT GATGCAGAAG AAAACACTCG
     7621 GCTGGGAGGC CTTCACCGAG ACGCTGTACC CCGCTGACGG CGGCCTGGAA GGCAGAAACG
     7681 ACATGGCCCT GAAGCTCGTG GGCGGGAGCC ATCTGATCGC AAACATCAAG ACCACATATA
     7741 GATCCAAGAA ACCCGCTAAG AACCTCAAGA TGCCTGGCGT CTACTATGTG GACTACAGAC
     7801 TGGAAAGAAT CAAGGAGGCC AACAACGAGA CCTACGTCGA GCAGCACGAG GTGGCAGTGG
     7861 CCAGATACTG CGACCTCCCT AGCAAACTGG GGCACAAGCT TAATggtgga ggaggttctg
     7921 gaggcggtgg aagtggtggc ggaggtagcG TCGACAGCAA GGGCGAGGAG CTGTTCACCG
     7981 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
     8041 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
     8101 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT
     8161 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
     8221 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
     8281 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
     8341 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
     8401 TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA
     8461 ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
     8521 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG
     8581 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
     8641 CTCTCGGCAT GGACGAGCTG TACAAGTAAT AGATGCATGG CCGGCCTTAA TTAAACGCGT
     8701 CTCGAGGGAA TTCCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA
     8761 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC
     8821 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT
     8881 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG
     8941 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT
     9001 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT
     9061 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT
     9121 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT
     9181 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC
     9241 GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT
     9301 CCCCGCATCG GGAATTCGAG CTCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA
     9361 GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA
     9421 AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
     9481 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
     9541 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
     9601 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATCTAGCTAG AATTAATTCC GTGTATTCTA
     9661 TAGTGTCACC TAAATCGTAT GTGTATGATA CATAAGGTTA TGTATTAATT GTAGCCGCGT
     9721 TCTAACGACA ATATGTACAA GCCTAATTGT GTAGCATCTG GCTTACTGAA GCAGACCCTA
     9781 TCATCTCTCT CGTAAACTGC CGTCAGAGTC GGTTTGGTTG GACGAACCTT CTGAGTTTCT
     9841 GGTAACGCCG TCCCGCACCC GGAAATGGTC AGCGAACCAA TCAGCAGGGT CATCGCTAGC
     9901 CTAGGCTTTT GCGTCGAGAC GTACCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT
     9961 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC
    10021 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC
    10081 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CGACGCGCCC TGTAGCGGCG
    10141 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
    10201 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
    10261 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
    10321 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
    10381 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
    10441 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
    10501 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA
    10561 TATTAACGTT TACAATTTCC CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT
    10621 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA
    10681 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT
    10741 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA
    10801 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA
    10861 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT
    10921 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG
    10981 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA
    11041 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA
    11101 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT
    11161 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC
    11221 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA
    11281 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA
    11341 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC
    11401 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA
    11461 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA
    11521 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA
    11581 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT
    11641 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT
    11701 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
    11761 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
    11821 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
    11881 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
    11941 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
    12001 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
    12061 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
    12121 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
    12181 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
    12241 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
    12301 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
    12361 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
    12421 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA
    12481 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC
    12541 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG
    12601 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA
    12661 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
    12721 AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC CCTCACTAAA GGGAACAAAA
    12781 GCTGGAGCTG C
//
