LOCUS       282_GFP_C1_SEC_3       10476 bp ds-DNA     circular     01-JUN-2015
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to mNG,
            or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    404..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2767..2907,2955..3788)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6097..7122
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1983..2012
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8657..9209)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9099..9206
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4161..4402,4454..4824,4876..5291))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8657..9245)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9063..9068
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9240..9245)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1929..1939
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9085..9090
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1940..1973
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9219..9224)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9419..10078)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1980..1982
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3018..3018
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9416..10276)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2016..2396
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1974..1979
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8251..8271)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2681..2708,2765..2766)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1902..1922
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1038..1340,1389..1463,1515..1634,1686..1901)
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff33
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8639..9321)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3789..3825
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5292..5691)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7606..7632
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2397..2766
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7579..7605
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7540..7551
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7552..7578
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3826..4157)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7534..7539
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7148..7499
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7500..7533
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5692..6054
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8240..8246
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7633..8239
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7677..7982)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7680..7889)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG AGTAAAGGAG AAGAATTGTT CACTGGAGTT GTCCCAATCC
     1081 TCGTCGAGCT CGACGGAGAC GTCAACGGAC ACAAGTTCTC CGTCTCCGGA GAGGGAGAGG
     1141 GAGACGCCAC CTACGGAAAG CTCACCCTCA AGTTCATCTG CACCACCGGA AAGCTCCCAG
     1201 TCCCATGGCC AACCCTCGTC ACCACCTTCT GCTACGGAGT CCAATGCTTC TCCCGTTACC
     1261 CAGACCACAT GAAGCGTCAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA TACGTCCAAG
     1321 AGCGTACCAT CTTCTTCAAG gtaagtttaa acatatatat actaactact gattatttaa
     1381 attttcagGA CGACGGAAAC TACAAGACCC GTGCCGAGGT CAAGTTCGAG GGAGACACCC
     1441 TCGTCAACCG TATCGAGCTC AAGgtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagGGAATC GACTTCAAGG AGGACGGAAA CATCCTCGGA CACAAGCTCG
     1561 AGTACAACTA CAACTCCCAC AACGTCTACA TCATGGCCGA CAAGCAAAAG AACGGAATCA
     1621 AGGTCAACTT CAAGgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1681 ttcagATCCG TCACAACATC GAGGACGGAT CCGTCCAACT CGCCGACCAC TACCAACAAA
     1741 ACACCCCAAT CGGAGACGGA CCAGTCCTCC TCCCAGACAA CCACTACCTC TCCACCCAAT
     1801 CCGCCCTCTC CAAGGACCCA AACGAGAAGC GTGACCACAT GGTCCTCCTC GAGTTCGTCA
     1861 CCGCCGCCGG AATCACCCAC GGAATGGACG AGCTCTACAA GGAGAATCTG TACTTTCAAT
     1921 CCGGAAAGgt aagtttaaaA TAACTTCGTA TAGCATACAT TATACGAAGT TATtttcagG
     1981 GAGAGCAAAA GCTTATCTCC GAGGAGGACC TCTAAGGATG ATCGACGCCa ACGTCGTTGA
     2041 ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT ATTATTTATT TATTCTCTTT
     2101 GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA AATCTAAaca tttttttttc
     2161 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2221 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2281 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2341 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aactagctgc
     2401 cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct
     2461 ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc
     2521 ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt
     2581 ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg
     2641 aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg
     2701 aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt
     2761 tcagagATGT CTGTAAAACT TGCGTGTTAT GTGACGGCTT CGGTCACTGT CGCCACTCTT
     2821 ATGGTATGCT TCATGACCAT GTCAACCATC TACTCGGAGG TTGATGGATT CAGAGAGAAG
     2881 CTTGATACCG AGATGAATGT GTTCAGAgtg agttaaatta tattttgaat tttaaataat
     2941 ttttaatttt ctagCAATCT ACCAATGGAT TGTGGAAGGA CATAGTTGTC ATCGGAAGAT
     3001 CTAGCAAGCG TGTCCGTTGT CAATATGAAG AGACCAACGC TACCCCAACT CCACATGCTG
     3061 ATGGATCCCC ATCTGCTCCA CCAGGTCAAC CACCAGCAGT TCCACCAGTC TTCAACCAGC
     3121 CAAAGACTCC AAATGGAGCC AATGGAAATG GACCAACCTG CAACTGCAAT GCTGATAACA
     3181 AGTGCCCAGC TGGACCATCC GGACCAAAGG GAGTTCCAGG AGTTCCAGGA CTCGACGGAG
     3241 TTCCAGGACT TGACGGTGTT CCAGGAGTTG GAGCTGATGA TATCGCTCCA CAACGCGAGT
     3301 CTGTCGGATG CTTCACTTGC CCACAAGGAC CAGTTGGACC ACCAGGAGCT CTTGGAAGAC
     3361 CAGGACCACG TGGACTTCCA GGACCAAGAG GACAAAATGG AAACCCAGGA AGAGATGGAC
     3421 AACCAGGACA TCCAGGAGAG CAAGGATCAT CCGGTCAAAT CGGAAAGATC GGAGAGCCAG
     3481 GACCACCAGG AGAGAAGGGA CGCGACGCCG AGCATCCAAT CGGAAGACCA GGACCAAAGG
     3541 GACCAAGAGG AGATCAAGGA CCAACAGGAC CAGCTGGACA GAACGGTCTT CACGGACCAC
     3601 CAGGAGAGCC AGGAACCGTT GGACCAGAAG GACCATCTGG AAAGCAAGGA CGTCAAGGAC
     3661 CAGACGGAAC CCAGGGAGAG ACTGGACCAG ACGGAAGACC AGGAAAGGAT GCCGAGTACT
     3721 GCCAGTGCCC AGACAAGTCT CCACCATCAG AGGCTGTCAA CGCCAACCGT GGATACAGAA
     3781 ATATCTAAat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt
     3841 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt
     3901 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaatg gaattgaaga
     3961 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg
     4021 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt
     4081 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa
     4141 aggattttgc atttatctta ATCACCGTCT TCGAGAAGAC GAACCATGGC GCCAGTTTCA
     4201 GAATCCAAGT TTCTAATATA GTTCATCACA ATGTTGACGT TGGTCCAACC TCCGGCTTGC
     4261 ATAATCTCTG GGATGGAAAC TCCAGCGCGA GCCATGTCAC GGGCAGCTCC GACACGGGCG
     4321 GAATGTCCAG ACCAAGCCAA GTAACGTTGT CCGGAATCAT CCTTGGCTCC GTAGATGAGA
     4381 CGGTGGGTAG CTTCGAAGAT TCctgaaaat ttattttcag cttattctta gctaatatgt
     4441 tgtttaaact tacCTTCAAG AGCTCGAGTC GAGAGTTGTG ATGTTGCACT CGGGGCAGCA
     4501 ACTCCGTTCT TGCGAACACG ACAGAACAGG TAGTTGTTTG GATCATCGGC GACTCCGGAA
     4561 ACCGAAATCC AGCGCTCAAC GAGCTTGGTA ACTCCAAGGG AGAGCGCTTT TTCGACTCCG
     4621 GCAGTCGAGA CCAGCGTTTT TGTACGTCCG ATGTGGATGA GCATTCTTCC TCCGTCTGTG
     4681 CGACTAATAT CCTTGACGCG AATGCGGGCA ATTTCGGCGA TACGGAGAAG TGTGTTGTAA
     4741 GCAATTCCGA GGAAGGCGAG GTTTCGGATG TCCTGGCAAC GATCGGAATT TTCCATAAGA
     4801 GAACGGACCT GATCGAAGTC GGTTctgaaa atttcgtcaa gtcttaggat tgaatacatt
     4861 aatatttaaa cttacCTTTC GAAGGCGAGG GCTTGCTTGG CTCTCTCTCC AGCATCAACA
     4921 TTTTCCTTGC GGATACGTCT CATTACGAGA GATACGGCAT TAGAATCTGA TGGGCGTGGA
     4981 AGTCCAGATC GGCGATGGAG CATATTGAGC TGTCCGAGAT GTTGTTGGAT CGTCTTCACC
     5041 GCCAATCCAC GAGCTTGGAG GTAGAGCAAG TAGTCACGAA CGTCTTCTGG CTCGGCTGGG
     5101 AACCACTTGC GGTTGTTAAG CTTGCACCAG GCAGCCCAAG AACGGCACAC TGAGAGAAGC
     5161 ATCTTCCAGG TGTGCTCGGA GAAGGCTTGT CTGTCACGGA ACATATCCAT GAGGTTCTTG
     5221 CGAACTTCAT CACTTGTCGC GTCAACTGGA AGTGCTGGAA GATTTTGATG AACAGTGAGA
     5281 AGATTTGACA Tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc
     5341 tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc
     5401 tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta
     5461 tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt
     5521 ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa
     5581 aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca
     5641 tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat cATTTTTGCT
     5701 TTCGTCGTAA ATCTACACAC GCGTCTCTTC CGTGCGAGAG TCCAAGCCAG CAGCCAAATT
     5761 CGTTGACTGA GTATTCAACG TTTATACGTT GTCGGCAACG AGAAATAGGA AAATGCATCG
     5821 GGAAATGTTC TTTTTTCGAT TTTTTCCAAG GTTTTGACAA ATTTTACCAC GAATTTTGCT
     5881 ATGTTTTCAA TTAAAAAATA TGTTATTCAA CTGTTTCTAT GAGGAAAATA AGGCTTTGCA
     5941 TGTAATTTTC TTATTCAGCA TAATTTTTAA TTAATTTGAA TTTTCTGTCC TAACGTTTAT
     6001 TTTGTTTTCT TGGTTATGAC TGATCTGAAA TTAATTTTTG AATTTTAAGG TAATATGTCA
     6061 GGCGGTGCCG CAAGTTTGTA CAAAAAAGCA GGCTCCATGA AAAAGCCTGA ACTCACCGCG
     6121 ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC
     6181 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG
     6241 CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA
     6301 TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC
     6361 TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG
     6421 CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC
     6481 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT
     6541 GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC
     6601 ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC
     6661 CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT
     6721 GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG
     6781 GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA GACGCGCTAC
     6841 TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC TCCGGGCGTA TATGCTCCGC
     6901 ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA ATTTCGATGA TGCAGCTTGG
     6961 GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA
     7021 ATCGCCCGCA GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT
     7081 GGAAACCGAC GCCCCAGCAC TCGTCCGAGG GCAAAGGAAT AGACCCAGCT TTCTTGTACA
     7141 AAGTGGGTCC AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC
     7201 CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG
     7261 TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC
     7321 GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG
     7381 TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA
     7441 AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAA
     7501 TAACTTCGTA TAGCATACAT TATACGAAGT TATtttcagG GAGCCGGATC TGATTATAAA
     7561 GACGATGACG ATAAGCGTGA CTACAAGGAC GACGACGACA AGCGTGATTA CAAGGATGAC
     7621 GATGACAAGA GActagtTCG ACCTGCAGAC TGGCTGTGTA TAAGGGAGCC TGACATTTAT
     7681 ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT TTTCGCGGTG GCTGAGATCA
     7741 GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA TATCGGTGGT CATCATGCGC
     7801 CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC GGGGGACTTT ATCTGACAGC
     7861 AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG GCGTGTCAAT AATATCACTC
     7921 TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT AGGTGTAAAC CTTAAACTGC
     7981 ATTTCACCAG CCCCTGTTCT CGTCGGCAAA AGAGCCGTTC ATTTCAATAA ACCGGGCGAC
     8041 CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG GCACGCAGAC GACGGGCTTC
     8101 ATTCTGCATG GTTGTGCTTA CCGAACCGGA GATATTGACA TCATATATGC CTTGAGCAAC
     8161 TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT TCATAGCATA CCTCTTTTTG
     8221 ACATACTTCG GGTtcctagg aaatcgataa catggtcata gctgtttcct gtgtgaaatt
     8281 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8341 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8401 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8461 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8521 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8581 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8641 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8701 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8761 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8821 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8881 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8941 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9001 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9061 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9121 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9181 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9241 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9301 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9361 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9421 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9481 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9541 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9601 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9661 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9721 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9781 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9841 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9901 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9961 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10021 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10081 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10141 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10201 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10261 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10321 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10381 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10441 cctataaaaa taggcgtatc acgaggccct ttcgtc  
//
