LOCUS       Chk1                    6048 bp    DNA     linear       14-MAY-2015
FEATURES             Location/Qualifiers
     misc_feature    1342..2061
                     /note="GFP"
     misc_feature    106..1335
                     /note="chk1 cdna"
     misc_feature    100..105
                     /note="kozak"
     misc_feature    871..873
                     /note="S256"
     misc_feature    943..945
                     /note="S280"
     misc_feature    979..981
                     /note="T292"
     misc_feature    1006..1008
                     /note="S301"
     source          1..6048
                     /dnas_title="Chk1 GFP pCS2+ 82013"
ORIGIN      
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCa ggaccATGGC TGTGCCTTTT
      121 GTTAAAGACT GGGATGTGGT ACAAACTCTT GGAGAGGGAG CATATGGAGA GGTGCGACTG
      181 CTGGTCAACA AGAAAACAGA AGAGGCTGTG GCGGTGAAAG TTGTGGACAT GGCAAAAGCC
      241 AAGGATTGCA TCGAGAATGT GAAGAAGGAG GTCTGCATAT GCAAGATGCT TTCACACCCC
      301 AACATTGTAC GTTTCTTTGG GCACAGTGTT GGGATTACAC ATCGTGACAT AAAGCCTGAG
      361 AATATTCTTC TTGATGATAA AGATAATCTG AAGATCTCTG ACTTTGGCCT GGCTACCATG
      421 TTCAGGCACC GTGGCCGTGA GCGAGCTTTG AACCGTCTGT GTGGTACTCT GCCCTATGTT
      481 GCCCCAGAGT TGATGTCACG CTCATCTTTT AACGCTCAGC CTGCGGACAC TTGGGCTTGT
      541 GGCATTGTGC TCACTGCAAT GTTAGCTGGA GAGTTACCCT GGGATCAGCC GAGTGAAAAC
      601 TGTCAGGAAT ATTTGGACTG GCTGGAAAGA AAGACCTACC TTACACCCTG GAAGAAAATT
      661 GATGCGGTAC CCCTTAGTCT GTTGTCTAAG ATATTACTGC ACAATCCAGA AGACAGGTTC
      721 ACCATTCCTG AAATTAAGAA ACACCGCTGG TTTAGCAGAA GTTTCAAATC AGCAGTACAA
      781 CGTCAGGGCA TCACACCAGC AGCCAAGATT CAACGGGCTG ACTCTGAGCT GAGACGAAAA
      841 AACAGTGATG ACCGAGCGCA GATCTCCAGC TCTCAGCCTG AGCCACAGGG ATTATGGGAG
      901 GAGAAGGAGG TTACAGTTCA CACCGATGTC AGTGTCAGCT TTTCACAACC GACCTGTCCT
      961 GACCACATGC TGCTGGGCAC ACAACTGCTT GGCACACCGG GTGCAAGCCA GAGCCCCTGG
     1021 CAGCGTTTGG TGAGGAGAAT GACCAGGTTC TTTACTACAG AAAATACAGA GGCATCTTGC
     1081 ATTGCTCTCC GTGATTCTTG TATTGCTTTG GGTCACACAT GGAAACAGAG CTGTAATAAT
     1141 CAGGCGACAG TGTCTACAAT GGATCGTCGC AATAATAAGC TGATATTCAT AGTGCACTTC
     1201 CTGGAAATGG AGGAACGTAT TTTAGTTGAT TTCCGGCTGT CAAAGGGGGA CGGTTTGGAA
     1261 TTCAAGCGAA TATTCCTGAA ACTCAAACAG AAGCTGTCTG ACATTATTAG CAACCAAAAG
     1321 GTTTTGCCAT TGATTCTCGA GATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
     1381 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
     1441 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
     1501 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC
     1561 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
     1621 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
     1681 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
     1741 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
     1801 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG
     1861 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
     1921 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
     1981 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
     2041 ATGGACGAGC TGTACAAGTA ACCTCTAGAT TCTGCAGCCC TATAGTGAGT CGTATTACGT
     2101 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
     2161 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
     2221 CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA
     2281 GGTGTGGGAG GTTTTTTAAT TCGCGGCCGC GGCGCCAATG CATTGGGCCC GGTACCCAGC
     2341 TTTTGTTCCC TTTAGTGAGG GTTAATTGCG CGCTTGGCGT AATCATGGTC ATAGCTGTTT
     2401 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
     2461 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
     2521 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
     2581 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
     2641 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     2701 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     2761 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     2821 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     2881 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     2941 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     3001 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     3061 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     3121 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     3181 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
     3241 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     3301 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     3361 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     3421 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     3481 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3541 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     3601 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     3661 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
     3721 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3781 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3841 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3901 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3961 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     4021 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     4081 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     4141 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     4201 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     4261 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     4321 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     4381 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     4441 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     4501 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTA AATTGTAAGC GTTAATATTT
     4561 TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA
     4621 TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG
     4681 TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG
     4741 TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA
     4801 GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG
     4861 GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG
     4921 CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC
     4981 CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG
     5041 TGCGGGCCTC TTCGCTATTA CGCCAGTCGA CCATAGCCAA TTCAATATGG CGTATATGGA
     5101 CTCATGCCAA TTCAATATGG TGGATCTGGA CCTGTGCCAA TTCAATATGG CGTATATGGA
     5161 CTCGTGCCAA TTCAATATGG TGGATCTGGA CCCCAGCCAA TTCAATATGG CGGACTTGGC
     5221 ACCATGCCAA TTCAATATGG CGGACTTGGC ACTGTGCCAA CTGGGGAGGG GTCTACTTGG
     5281 CACGGTGCCA AGTTTGAGGA GGGGTCTTGG CCCTGTGCCA AGTCCGCCAT ATTGAATTGG
     5341 CATGGTGCCA ATAATGGCGG CCATATTGGC TATATGCCAG GATCAATATA TAGGCAATAT
     5401 CCAATATGGC CCTATGCCAA TATGGCTATT GGCCAGGTTC AATACTATGT ATTGGCCCTA
     5461 TGCCATATAG TATTCCATAT ATGGGTTTTC CTATTGACGT AGATAGCCCC TCCCAATGGG
     5521 CGGTCCCATA TACCATATAT GGGGCTTCCT AATACCGCCC ATAGCCACTC CCCCATTGAC
     5581 GTCAATGGTC TCTATATATG GTCTTTCCTA TTGACGTCAT ATGGGCGGTC CTATTGACGT
     5641 ATATGGCGCC TCCCCCATTG ACGTCAATTA CGGTAAATGG CCCGCCTGGC TCAATGCCCA
     5701 TTGACGTCAA TAGGACCACC CACCATTGAC GTCAATGGGA TGGCTCATTG CCCATTCATA
     5761 TCCGTTCTCA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCACTTG GCAGTACATC
     5821 AATATCTATT AATAGTAACT TGGCAAGTAC ATTACTATTG GAAGGACGCC AGGGTACATT
     5881 GGCAGTACTC CCATTGACGT CAATGGCGGT AAATGGCCCG CGATGGCTGC CAAGTACATC
     5941 CCCATTGACG TCAATGGGGA GGGGCAATGA CGCAAATGGG CGTTCCATTG ACGTAAATGG
     6001 GCGGTAGGCG TGCCTAATGG GAGGTCTATA TAAGCAATGC TCGTTTAG
//

