LOCUS Exported 6554 bp ds-DNA circular SYN 17-AUG-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMAL-c5X-ELP[V-60] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6554) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Aug 17, 2015 from SnapGene 2.8.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6554 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 3..80 /label=lacIq promoter /note="lacIq promoter" CDS 81..1163 /label=lacI /note="lacI" promoter 1406..1411 /label=tac promoter /note="tac promoter" promoter 1412..1427 /label=tac promoter /note="tac promoter" promoter 1428..1434 /label=tac promoter /note="tac promoter" protein_bind 1442..1458 /label=lac operator /note="lac operator" CDS 1528..2628 /label=MBP /note="MBP" CDS 2677..2688 /label=Factor Xa site /note="Factor Xa site" CDS 2698..3597 /label=ELP[V-60] /note="ELP[V-60]" misc_feature 3607..3624 /label=His6 tag /note="His6 tag" terminator 3640..3726 /label=rrnB T1 terminator /note="rrnB T1 terminator" terminator 3818..3845 /label=rrnB T2 terminator /note="rrnB T2 terminator" promoter 3873..3963 /label=AmpR promoter /note="AmpR promoter" CDS 3964..4032 /label=AmpR /note="AmpR" CDS 4033..4824 /label=AmpR /note="AmpR" rep_origin 4912..5500 /label=ori /note="ori" CDS complement(5870..6061) /label=rop /note="rop" ORIGIN 1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcaattgacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg 1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat 1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt 1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac 1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat 1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt 1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa 1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac 1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag 2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg 2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac 2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg 2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc 2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt 2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg 2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag 2461 ttggtgaaag atccgcgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg 2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc 2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg 2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg aaggcaggtc ttccggtggc 2701 gttggggtac ccggggtagg tgttcccggt gttggagttc ccggggtcgg tgtcccaggg 2761 gtcggagtgc ctggcgtggg tgtacccggt gtcggggttc caggtgtcgg tgtgcctgga 2821 gtaggtgtcc ctggcgttgg cgtcccagga gtgggcgtcc ctggggttgg tgttcctgga 2881 gttggcgtac cgggggtggg ggtacctgga gtgggagtgc cgggtgttgg tgtgcccggt 2941 gtgggtgtcc ccggagttgg ggttcctggt gttggcgtgc ctggtgtcgg cgttccgggt 3001 gtgggggtcc ctggtgtggg cgttcccggc gtaggtgtac cgggtgtcgg agttccggga 3061 gtaggcgtac ctggggtcgg ggtaccgggc gttggtgtac caggagttgg agtgccaggc 3121 gtcggagtac ccggagtcgg agtcccgggt gtaggtgtgc cgggggttgg cgttcctggg 3181 gtgggcgtac caggggtggg agtacctggt gtaggcgtgc caggtgtggg agttcctggc 3241 gtcggtgttc cgggcgtagg ggtgcctggg gtaggggtac caggtgttgg ggtgccggga 3301 atgggtgttc caggcgtggg ggtgcccggg gttggggtcc caggtgtagg ggtccccggg 3361 gtgggtgtgc caggagtcgg ggtgccaggg gttggagtac caggcgttgg agtccctgga 3421 gtcggcgtac ccggcgtggg agtcccaggc gtaggcgtcc ccggtgtagg agtaccggga 3481 gttggtgtcc cgggagtcgg tgtacctggc gtaggagttc caggggtagg cgttccagga 3541 gtaggagtgc ccggagtagg ggttcccgga gtgggggttc cgggggtagg agtccccggc 3601 tcgggacatc atcaccacca tcattaataa tgaaagcttc aaataaaacg aaaggctcag 3661 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg 3721 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca 3781 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc 3841 ctttttgcgt ttctacaaac tctttcggtc cgttgtttat ttttctaaat acattcaaat 3901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 3961 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 4021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 4081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 4141 cccgaagaac gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 4201 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 4261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 4321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 4381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 4441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 4501 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 4561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 4621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 4681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 4741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 4801 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 4861 gatttcctta ggactgagcg tcaaccccgt agaaaagatc aaaggatctt cttgagatcc 4921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 4981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5041 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 5101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 5161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 5221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 5581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 5641 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 5701 tctccttacg catctgtgcg gtatttcaca ccgcatataa ggtgcactgt gactgggtca 5761 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 5821 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 5881 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca 5941 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg 6001 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca 6061 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag 6121 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg 6181 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat 6241 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga 6301 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa 6361 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt 6421 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc 6481 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa 6541 cgctgcccga aatt //