LOCUS       Exported                5178 bp ds-DNA     circular SYN 31-AUG-1994
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    pCellFree_G09
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Dejan Gagoski
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 18, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..25
                     /note="T7_promoter"
     misc_feature    37..102
                     /note="PolyUA"
     misc_feature    103..156
                     /note="SITS"
     misc_feature    169..192
                     /note="8xHis"
     CDS             199..912
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    913..936
                     /note="Precission CS"
     misc_feature    937..1061
                     /note="attR1"
     misc_feature    complement(1108..1413)
                     /note="ccdB"
     CDS             complement(1755..2414)
                     /codon_start=1
                     /note="ORF frame 3"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(1755..2414)
                     /gene="CAT/CamR"
                     /note="CAT/CamR"
     misc_feature    2659..2679
                     /note="attR2*"
     terminator      2908..2943
                     /note="T7_terminator"
     promoter        complement(3023..3041)
                     /note="M13_reverse_primer"
     misc_feature    3040..3062
                     /note="M13_pUC_rev_primer"
     promoter        complement(3076..3105)
                     /note="lac_promoter"
     CDS             complement(4188..5048)
                     /codon_start=1
                     /note="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERTRQIAEIGAS
                     LIKHW"
     gene            complement(4188..5048)
                     /gene="Ampicillin"
                     /note="Ampicillin"
     promoter        complement(5090..5118)
                     /note="AmpR_promoter"
ORIGIN
        1 gacgtctaat acgactcact atagggacat cttaagttta ttttatttta ttttatttta
       61 ttttatttta ttttatttta ttttatttta ttttatttaa ccatgacagt aatgtataaa
      121 gtctgtaaag acattaaaca cgtaagtgaa accatgggca cctctggtca ccaccatcac
      181 caccatcacc acggcacaat gagcaaagga gaagaacttt tcactggagt tgtcccaatt
      241 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa
      301 ggtgatgcta caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct
      361 gttccgtggc caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat
      421 ccggatcaca tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      481 gaacgcacta tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt
      541 gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga
      601 aacattcttg gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca
      661 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt
      721 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      781 ttaccagaca accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag
      841 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
      901 gagctctaca aactggaggt gctcttccag ggtccgacaa gtttgtacaa aaaagctgaa
      961 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1021 actacataat actgtaaaac acaacatatc cagtcactat ggggcccgtc gacctgcaga
     1081 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
     1141 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
     1201 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
     1261 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
     1321 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
     1381 ctctctttta taggtgtaaa ccttaaactg catttcacca gtccctgttc tcgtcagcaa
     1441 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
     1501 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
     1561 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
     1621 aatacgctgc ttcatagcac acctcttttt gacatacttc gggtatacat atcagtatat
     1681 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
     1741 ccggatccac gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1801 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
     1861 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1921 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1981 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2041 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2101 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2161 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     2221 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2281 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2341 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2401 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     2461 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     2521 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     2581 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     2641 gatgctgcca acttagcaag ctttcttgta caaagtggtg taagcggccg ccctcctcct
     2701 cctttcttgt tcctttcacg tcgccttctc ggttgtagct ggcagacgac gagtcttact
     2761 tttacgtgta cttctctata gatgatgtat gatctctctg catgcgtgtt cgtgcatgtg
     2821 tccgtgtgtt gggtacgcgt ggtaccctgc aggaaggaag ctgagttggc tgctgccacc
     2881 gctgagcaat aactagtaat tactagcata accccttggg gcctctaaac gggtcttgag
     2941 ggggtttttt gctgaaagga ggacagctga tgattgtcat gcttgccatc tgttttcttg
     3001 caaggtcaga ggaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3061 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3121 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3181 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3241 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3421 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3841 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagag
     4021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4201 atcagtgagg cacctatctc agcgatctgt ctagttcgtt catccatagt tgcctgactc
     4261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5161 ccccgaaaag tgccacct
//
