LOCUS       Exported                9662 bp ds-DNA     circular SYN 12-JUN-2015
DEFINITION  Conjugally transferable red recombinase vector pMJH46, complete 
            sequence.
ACCESSION   JQ070344
VERSION     .
KEYWORDS    JQ070344.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Hossain MJ, Liles MR.
  TITLE     Highly Efficient Markerless Deletion of Edwardsiella ictaluri genes 
            by Recombineering
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Hossain MJ, Liles MR.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2011) Biological Sciences, Auburn University, 120 
            W Samford Avenue, Auburn, AL 36830, USA
REFERENCE   3  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2015 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Conjugally transferable red recombinase vector 
                     pMJH46"
                     /lab_host="Escherichia coli SM10lambdapir"
                     /mol_type="other DNA"
                     /db_xref="taxon:1162228"
     CDS             complement(11..889)
                     /codon_start=1
                     /transl_table=11
                     /product="AraC protein"
                     /note="AraC protein"
                     /db_xref="GI:386436494"
                     /protein_id="AFJ11900.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        916..1200
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1244..1660
                     /codon_start=1
                     /transl_table=11
                     /product="Gam protein"
                     /note="Gam protein"
                     /note="recombination;138"
                     /db_xref="GI:386436495"
                     /protein_id="AFJ11901.1"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1666..2451
                     /codon_start=1
                     /transl_table=11
                     /product="Bet protein"
                     /note="Bet protein"
                     /note="recombination;261"
                     /db_xref="GI:386436496"
                     /protein_id="AFJ11902.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2448..3128
                     /codon_start=1
                     /transl_table=11
                     /product="Exonuclease"
                     /note="Exonuclease"
                     /note="exo"
                     /db_xref="GI:386436497"
                     /protein_id="AFJ11903.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     misc_feature    3118..3387
                     /note="tL3"
     CDS             3125..3307
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /note="hypothetical protein"
                     /note="orf60a protein"
                     /db_xref="GI:386436498"
                     /protein_id="AFJ11904.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEEI
                     NNKRGAVCTKHLLLS"
     terminator      3129..3373
                     /note="lambda tL3 terminator"
                     /note="transcription terminator tL3 from phage lambda"
     oriT            4236..4345
                     /note="incP origin of transfer"
     CDS             4378..4749
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome component TraJ"
                     /note="conjugal transfer relaxosome component TraJ"
                     /note="involved in conjugative DNA transfer; initiation of 
                     relaxosome assembly; recognition and binding to the oriT"
                     /db_xref="GI:386436499"
                     /protein_id="AFJ11905.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     promoter        5795..5897
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5898..6557
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /note="chloramphenicol acetyltransferase"
                     /note="cat; chloramphenicol resistance"
                     /db_xref="GI:386436500"
                     /protein_id="AFJ11906.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(6829..7779)
                     /codon_start=1
                     /transl_table=11
                     /gene="repA101"
                     /product="RepA101 protein"
                     /note="repA101"
                     /db_xref="GI:386436501"
                     /protein_id="AFJ11907.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            complement(6829..7779)
                     /gene="repA101"
                     /note="repA101"
     rep_origin      7783..8523
                     /note="oriR101"
     rep_origin      7827..8049
                     /note="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(8671..9531)
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /note="beta-lactamase"
                     /db_xref="GI:386436502"
                     /protein_id="AFJ11908.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9532..9636)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
     1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
     1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
     1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
     1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
     1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
     1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
     1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
     1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
     1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
     1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
     1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
     1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
     1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
     2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
     2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
     2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
     2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
     2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
     2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
     2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
     2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
     2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
     2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
     2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
     2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
     2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
     2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
     2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
     2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
     3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
     3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
     3121 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt
     3181 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca
     3241 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt
     3301 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg
     3361 ccaataccag tagaaacaga cgaagaatcc atgatcaccg ctaacctgtc ttttaacctg
     3421 cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac
     3481 cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc
     3541 ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca
     3601 ggggatctcg agggatcctt tttgtccggt gttgggttga aggtgaagcc ggtcggggcc
     3661 gcagcggggg ccggcttttc agccttgccc ccctgcttcg gccgccgtgg ctccggcgtc
     3721 ttgggtgccg gcgcgggttc cgcagccttg gcctgcggtg cgggcacatc ggcgggcttg
     3781 gccttgatgt gccgcctggc gtgcgagcgg aacgtctcgt aggagaactt gaccttcccc
     3841 gtttcccgca tgtgctccca aatggtgacg agcgcatagc cggacgctaa cgccgcctcg
     3901 acatccgccc tcaccgccag gaacgcaacc gcagcctcat cacgccggcg cttcttggcc
     3961 gcgcgggatt caacccactc ggccagctcg tcggtgtagc tctttggcat cgtctctcgc
     4021 ctgtcccctc agttcagtaa tttcctgcat ttgcctgttt ccagtcggta gatattccac
     4081 aaaacagcag ggaagcagcg cttttccgct gcataaccct gcttcggggt cattatagcg
     4141 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     4201 gtagactttc cttggtgtac ccaacggcgt cagccgggca ggataggtga agtaggccca
     4261 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     4321 aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg
     4381 gctgatgaaa ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca
     4441 gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac
     4501 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc
     4561 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc
     4621 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag
     4681 atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca
     4741 gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt
     4801 ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga
     4861 gcaaggcaag accgagcgcc tgggtcacgt gcgcgtcacg aactgcgagg caatcaccct
     4921 gcccgctgtc atggccgagg tgatggcgac ccagcacggc aacacccgtt ccgaggccga
     4981 caagacctat cacctgctgg ttagcttccg cgcgggagag aagcccgacg cggagacgtt
     5041 gcgcgcgatt gaggaccgca tctgcgctgg gcttggcttc gccgagcatc agcgcgtcag
     5101 tgccgtgcat cacgacaccg acaacctgca catccatatc gccatcaaca agattcaccc
     5161 gacccgaaac accatccatg agccgtatcg ggcctaccgc gccctcgctg acctctgcgc
     5221 gacgctcgaa cgggactacg ggcttgagcg tgacaatcac gaaacgcggc agcgcgtttc
     5281 cgagaaccgc gcgaacgaca tggagcggca cgcgggcgtg gaaagcctgg tcggctgctc
     5341 gagggcttcg ccctgtcgct cgactgcggc gagcactact ggctgtaaaa ggacagacca
     5401 catcattatc gtgactgact gctgcgtgta gacttccgtt gaactgatgg acttatgtcc
     5461 catcaggctt tgcagaactt tcagcggtat accggcatac agcatgtgca tcgcatagga
     5521 atggcggaac gtatgtggtg tgaccggaac agagaacgtc acaccgtcag cagcagcggc
     5581 ggcaaccgcc tccccaatcc aggtcctgac cgttctgtcc gtcacttccc agatccgcgc
     5641 tttctctgtc cttcctgtgc gacggttacg ccgctccatg agcttatcgc gaataaatac
     5701 ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga
     5761 agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact
     5821 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     5881 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     5941 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     6001 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     6061 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     6121 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     6181 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     6241 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     6301 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     6361 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     6421 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     6481 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     6541 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct
     6601 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgatgata agctgtcaaa
     6661 catgagaatt ggtcgacggc ccgggcggcc gcaaggggtt cgcgttggcc gattcattag
     6721 atcatgggta tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg
     6781 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     6841 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     6901 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     6961 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttac gtaggtagga
     7021 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     7081 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     7141 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     7201 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     7261 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     7321 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     7381 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     7441 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     7501 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     7561 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg
     7621 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag
     7681 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     7741 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     7801 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
     7861 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
     7921 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
     7981 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
     8041 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt
     8101 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc
     8161 ggttgcggcc gcaatcgggc aaatcgctga atattccttt tgtctccgac catcaggcac
     8221 ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat
     8281 gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact acccataata
     8341 caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct
     8401 atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg
     8461 attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc
     8521 aacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9661 tg
//
