LOCUS       3130_pLV_hPGK_As        7779 bp ds-DNA     circular     05-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        4622..5137
                     /vntifkey="29"
                     /label=hPGK
                     /ApEinfo_fwdcolor=#804000
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4459..4582
                     /vntifkey="21"
                     /label=PPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             6520..6753
                     /vntifkey="19"
                     /label=dR3RU5
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3734..3974
                     /vntifkey="4"
                     /label=RRE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     insertion_seq   3976..4443
                     /vntifkey="14"
                     /label=denvRF2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7321..7779
                     /vntifkey="21"
                     /label=phageF1\origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_region   7160..7320
                     /vntifkey="34"
                     /label=pUC19
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1..1999
                     /vntifkey="88"
                     /label=pUC19(1)
                     /ApEinfo_label=pUC19
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2217..2889
                     /vntifkey="30"
                     /label=CMV\IE-I\prom
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     LTR             2891..3072
                     /vntifkey="19"
                     /label=RU5
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     insertion_seq   3588..3733
                     /vntifkey="14"
                     /label=denvRF1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3224..3588
                     /vntifkey="21"
                     /label=SL4\mgag
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3073..3223
                     /vntifkey="4"
                     /label=PBS\SL123
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        6954..7159
                     /vntifkey="30"
                     /label=SV40polyA\oriR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer          2989..3008
                     /vntifkey="27"
                     /label=SK-LTR4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer          6671..6690
                     /vntifkey="27"
                     /label=SK-LTR4(1)
                     /ApEinfo_label=SK-LTR4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5884..6395
                     /vntifkey="21"
                     /note="86bp downstream SacII-site removed by linker insert
                     (partial homology retained though)"
                     /label=WPRE*
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer          6247..6266
                     /vntifkey="27"
                     /label=WPREsense
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer          complement(6467..6486)
                     /vntifkey="27"
                     /label=#1000ASMIRTseq
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5161..5856
                     /label=ms Ascl1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5224..5224
                     /label=a in seq
                     /ApEinfo_fwdcolor=#54ceff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5556..5556
                     /label=c in seq
                     /ApEinfo_fwdcolor=#42bfff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct
     3121 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3181 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3241 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3301 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3361 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3421 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3481 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3541 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3601 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3661 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3721 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3781 cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3841 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3901 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3961 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     4021 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4081 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4141 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4201 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4261 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4321 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4381 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4441 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     4501 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     4561 tacaaaaatt caaaatttta tcgatcacga gactagcctc gagaagcttg atatcgaatt
     4621 cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     4681 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     4741 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     4801 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     4861 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     4921 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcggg gcgcgccgag
     4981 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     5041 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     5101 tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccggt gctagccacc
     5161 atggagagct ctggcaagat ggagagtgga gccggccagc agccgcagcc cccgcagccc
     5221 ttcctgcctc ccgcagcctg cttctttgcg accgcggcgg cggcggcagc ggcggcggcc
     5281 gcggcagctc agagcgcgca gcagcaacag ccgcaggcgc cgccgcagca ggcgccgcag
     5341 ctgagcccgg tggccgacag ccagccctca gggggcggtc acaagtcagc ggccaagcag
     5401 gtcaagcgcc agcgctcgtc ctctccggaa ctgatgcgct gcaaacgccg gctcaacttc
     5461 agcggcttcg gctacagcct gccacagcag cagccggccg ccgtggcgcg ccgcaacgag
     5521 cgcgagcgca accgggtcaa gttggtcaac ctgggttttg ccaccctccg ggagcatgtc
     5581 cccaacggcg cggccaacaa gaagatgagc aaggtggaga cgctgcgctc ggcggtcgag
     5641 tacatccgcg cgctgcagca gctgctggac gagcacgacg cggtgagcgc tgcctttcag
     5701 gcgggcgtcc tgtcgcccac catctccccc aactactcca acgacttgaa ctctatggcg
     5761 ggttctccgg tctcgtccta ctcctccgac gagggatcct acgaccctct tagcccagag
     5821 gaacaagagc tgctggactt taccaactgg ttctgagaat tcgatatcaa gcttatcgat
     5881 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5941 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6001 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6061 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6121 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6181 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6241 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6301 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6361 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctcta gagcctcttc
     6421 cgcgtcttcg ccttcccggg tcgagctcgg tacctttaag accaatgact tacaaggcag
     6481 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6541 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6601 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6661 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6721 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6781 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6901 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6961 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7021 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7081 aggaggcttt tttggaggcc taggcttttg cgtcgagacg tacccaattc gccctatagt
     7141 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7201 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7261 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     7321 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7381 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7621 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7681 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7741 aacgcgaatt ttaacaaaat attaacgttt acaatttcc  
//
