LOCUS       pCfB2188\(X-2-loxP-KlURA3syn)        6049 bp    DNA     circular     21-SEP-2015
DEFINITION  PCR product of p0126 (pX-2).
KEYWORDS    PCR_PRODUCT.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|690734139|
COMMENT     VNTDBDATE|745580471|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2188 (X-2-loxP-KlURA3syn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 2440 0 5266 0 0 "") 
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COMMENT        #13=(CFSignal (CObList) "loxP" 21 0 0 1736 1789 0 (CStringList) 
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FEATURES             Location/Qualifiers
     CDS             complement(3969..4827)
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      3223..3866
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    1934..2423
                     /vntifkey="21"
                     /label=X-2\Down
     terminator      30..219
                     /vntifkey="43"
                     /label=T\CYC1
     terminator      complement(5855..6049)
                     /vntifkey="43"
                     /label=T\ADH1
     misc_feature    5286..5816
                     /vntifkey="21"
                     /label=X-2\UP
     gene            complement(3969..4827)
                     /vntifkey="60"
     terminator      311..433
                     /vntifkey="43"
                     /label=K.\lactis\URA3\terminator
     promoter        complement(1238..1735)
                     /vntifkey="29"
                     /label=K.\lactis\URA3\promoter
     misc_feature    1736..1789
                     /vntifkey="21"
                     /label=loxP
     CDS             complement(434..1237)
                     /gene="URA3"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="AAG34531.1"
                     /db_xref="GI:11344893"
                     /vntifkey="4"
                     /label=K.\lactis\URA3
     misc_feature    251..310
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
     misc_feature    1151..1151
                     /vntifkey="21"
                     /label=Point\mutation\II\to\eliminate\BbsI\restriction\site
     misc_feature    779..779
                     /vntifkey="21"
                     /label=Point\Mutation\I\to\eliminate\BbsI\site
BASE COUNT     1647 a      1386 c      1251 g      1765 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatcccaata caacagatca cgtgatcttt ttgtaagatg aagttgaagt 
      361 agtgttgcac cgtgccaatg caggtggcta ttagattaaa tatgtgattt gttctattaa 
      421 gtttcctgta taattaatgg ggagcgctga ttctcttttg gtacgcttcc catccagcat 
      481 ttctgtatct ttcaccttca accttaggat ctctaccctt ggcgaaaagt cctctgccaa 
      541 caatgatgat atctgatcca ccacttacaa cttcgtcgac ggttctgtac tgctgaccca 
      601 atgcatcgcc tttgtcgtct aaacctacac ctggggtcat gattagccaa tcaaaccctt 
      661 cttctcttcc tcccatatcg ttctgagcaa tgaacccaat aacgaaatct ttatcactct 
      721 ttgcaatatc aacggtaccc ttagtatatt caccgtgtgc tagagaaccc ttggaagata 
      781 attcagcaag catcaataat ccccttggtt ctttggtgac ctcttgcgca ccttgtttca 
      841 agccagcaac aataccagca ccagtaaccc cgtgggcgtt ggtgatatca gaccattctg 
      901 cgatacggta aacgcccgat gtatattgta atttgactgt gttaccgata tcggcgaatt 
      961 ttctgtcctc aaatatcaag aacttgtatt tctctgccaa tgctttcaat ggaacgacag 
     1021 taccctcata actgaaatca tccaagatat caacgtgtgt tttcaaaagg caaatgtatg 
     1081 gacccaacgt ttcaacaagt ttcaatagct catcagtcga acgaacgtca agagaagcac 
     1141 acaaattggt tttcttttca tccattaaac gtaaaagttt cgatgcaacc ggacttgcat 
     1201 gagtctcagc tctactggta tatgattttg tggacatggt gcaactaatt gacgggagtg 
     1261 tattgacgct ggcgtactgg ctttcacaaa atggcccaat cacaaccaca tcttagatag 
     1321 ttgaaatgac tttagataac atcaattgag atgagcttaa tcatgtcaaa gctaaaagtg 
     1381 tcaccatgaa cgacaattct taagcaaatc acgtgatata gatccacgaa taaccaccat 
     1441 ttgatgctcg aggcaagtaa tgtgtgtaaa aaaatgcgtt accaccatcc aatgcagacc 
     1501 gatcttctac ccagaatcac atatatttat gtaccgagta ccttttttct atcttccaat 
     1561 tgcttctccc atatgattgt ctccgtaagc tcgaaatttc taagttggat tttaatcttc 
     1621 acgcaggatg acagttcgat gagcttctga ggagtgttta gaacataatc atttatccat 
     1681 ggtctatctc ttcttgtcgc tttttctcct cgatagaacc taaataaaac gagctctcga 
     1741 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt gatatcagat 
     1801 ccactagtgg cctatgcacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 
     1861 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccctgc aggactagtg 
     1921 ctgaggcatt aatcctgcat aatcggcctc acagagggat cccgttaccc atctatgctg 
     1981 aagatttatc atactattcc tccgctcgtt tcttttttca gtgaggtgtg tcgtgaaaga 
     2041 aaacccacaa ttaaactttc aacaaccggg cgactaggaa gagagtagtg ggcgcggatg 
     2101 acgaaggcta aggtcacttc ttcgtttcct ttattggggt ttccgtgtag ccttcccctg 
     2161 aatagtgtgg gacgttttat gagaagccgt aagaaatagg caaattgagt tatgacaagt 
     2221 agacatgatg ccgcagcctt gcctgacttt acgtctcctt catgaataag tttttctatc 
     2281 gagttctttt ccttttttcg ccttaattag ctcaattaag cctgtcctca ctacttttct 
     2341 ttttcttatc ggctttgtgc cacacctaac cttcgaatgc tgttttattc cgttcttaca 
     2401 tgggatggta atgccttggc gagattaaga cctcagcgcg gccgcaaatt taaataaaat 
     2461 gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc gaataagggc 
     2521 gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag tttgtattat 
     2581 attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta 
     2641 ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa 
     2701 atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta 
     2761 tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca 
     2821 agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca 
     2881 taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc 
     2941 agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac agaaagagtt 
     3001 tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat gcctggcagt 
     3061 tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     3121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     3181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     3241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     3301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     3361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     3421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     3481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     3541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     3601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     3661 ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 
     3721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     3781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     3841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgcgcgta 
     3901 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct 
     3961 ctgcttttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     4021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     4081 tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg atttatcagc 
     4141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     4201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     4261 gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     4321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     4381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     4441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     4561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     4621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     4681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     4741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4801 ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt gaagcattta 
     4861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     4921 aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc 
     4981 tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg 
     5041 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc 
     5101 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 
     5161 gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag tgaagttcct 
     5221 attctctaga aagtatagga acttctgaag tggggattta aatgcggccg cgctgagggt 
     5281 ttaatcgtct atgaggagac tgttagttgg atatcagtaa tgagacgaaa aagctcgaaa 
     5341 tgaatggata tattcttttt gctactggca actgttgaat atttaatgtt aaaacaaact 
     5401 aactgaggta tattcgtatc tgtatgtaca catatactat atacaggaaa agataagcaa 
     5461 gagagaggat atcaactacg agagcgatcg attatatatc aaaagctgtc cgctttgcca 
     5521 cccataatcg gcgcttagtt tcggagttca atcataattc taccacctta cactcaactt 
     5581 actctttaac tcctatagta taatatcgcc actgacccca tattaaaaaa tttttttgct 
     5641 cgatcttcta tcctctttag gttaattgtc gctgttattg tctagatttt ttctcggaga 
     5701 tggcgcatct atttgccgtc aaaagatcct ctcataccat attaagtaaa ttgcctccat 
     5761 ttctttttcc tcgggcagag aaactcgcag gcaacttgct ctcgaagtgg tcacgtatta 
     5821 agtcctcagc gagctcgcat ggaatgcgtg cgatgagcga cctcatgcta tacctgagaa 
     5881 agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa 
     5941 gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc 
     6001 ataaatcata agaaattcgc ttatttagaa gtgtcaacaa cgtatctac 
//
