LOCUS pCfB2226\(X-4-loxP-SpHIS5syn) 5864 bp DNA circular 1-APR-2014
DEFINITION PCR product of p2070 (pX-4_no_BsaI).
KEYWORDS PCR_PRODUCT.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
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COMMENT VNTDBDATE|694086164|
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COMMENT VNTNAME|pCfB2226 (X-4-loxP-SpHIS5syn)|
COMMENT VNTAUTHORNAME|Irina Borodina|
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FEATURES Location/Qualifiers
CDS complement(3793..4651)
/vntifkey="4"
/label=AmpR
rep_origin 3047..3690
/vntifkey="33"
/label=pUC\ori
misc_feature 1797..2247
/vntifkey="21"
/label=X-4\Down
terminator 30..219
/vntifkey="43"
/label=T\CYC1
terminator complement(5670..5864)
/vntifkey="43"
/label=T\ADH1
misc_feature 5110..5631
/vntifkey="21"
/label=X-4\UP
gene complement(3793..4651)
/vntifkey="60"
misc_feature 267..300
/vntifkey="21"
/label=loxP
promoter 301..698
/vntifkey="30"
/label=P\TEF
CDS 699..1352
/gene="HIS5"
/codon_start=1
/transl_table=11
/product="imidazoleglycerol-phosphate dehydratase"
/protein_id="AAG34536.1"
/db_xref="GI:11344900"
/vntifkey="4"
/label=HIS5
terminator 1353..1614
/vntifkey="43"
/label=T\TEF
misc_feature 1615..1648
/vntifkey="21"
/label=loxP
misc_feature 1..29
/vntifkey="21"
/label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT 1619 a 1323 c 1298 g 1624 t
ORIGIN
1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga
61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg
241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag
721 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac
781 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag
841 aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat catgcactgg
901 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc
961 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta
1021 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gctctttcta
1081 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg
1141 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag
1201 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg
1261 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt
1321 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt
1381 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
1441 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
1501 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
1561 gattcgatac taacgccgcc atccagttta aacgagctct cgagaaccct taatataact
1621 tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc
1681 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
1741 tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc attaataaca
1801 ggcatgggaa gattcgcttt ttttttttga attacaatag tatgtctgat gtctgcaaga
1861 agtaacaggc gtgtgcacaa gaatacgtgt gtgtgcgtaa gcgtatgcac tggtggcata
1921 acttatctaa gaagtatata tcactgacat agaaatgtag atatacaggt atttttctcg
1981 ataatcgata aaaatctcgt cgcgctgaac caaacttggt ggttacggag agtttttctc
2041 tcatcattac tgtctttcgc attgatttcc cctttgaccg ataaaatccc ttggattcat
2101 aagattaaac aaagaggtga tcaaagagaa ccctgtgaaa gtttatgttt ataaccgggc
2161 ataaagtgaa ctagacactt tcaagaagcc aaccaaagca tgagtaacga agcttaccag
2221 catgatcata ccgtaaatcc tcaccagatt aagacctcag cgcggccgca aatttaaata
2281 aaatgaagtg aagttcctat actttctaga gaataggaac ttctatagtg agtcgaataa
2341 gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt atagtttgta
2401 ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt
2461 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca
2521 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa
2581 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat
2641 atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc ctaaactccc
2701 cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac gattactcgt
2761 tatcagaacc gcccaggggg cccgagctta agactggccg tcgttttaca acacagaaag
2821 agtttgtaga aacgcaaaaa ggccatccgt caggggcctt ctgcttagtt tgatgcctgg
2881 cagttcccta ctctcgcctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
2941 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
3001 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
3061 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
3121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
3181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
3301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
3421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
3481 gttcttgaag tggtgggcta actacggcta cactagaaga acagtatttg gtatctgcgc
3541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
3661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgcg
3721 cgtaactcac gttaagggat tttggtcatg agcttgcgcc gtcccgtcaa gtcagcgtaa
3781 tgctctgctt ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
3841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
3901 catctggccc cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat
3961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
4021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
4081 gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta
4141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
4201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
4261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
4321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
4381 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
4441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
4501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
4561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
4621 taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca
4681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
4741 aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta
4801 tacctgaata tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca
4861 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact
4921 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
4981 ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt
5041 tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga
5101 gggtttaatc ccaaagctaa gagtcccatt ttattcttct atatgtatat tttcgatact
5161 ctaaaccacc ctacaatgta gccctatact aaatctgctc aattttcagc ttctacaagt
5221 gactcgaggc cacgtggaaa gatccaacta ctccagcaca acgattcaat ataatcgatt
5281 gctccactca taagaggcaa gaacaagctt caacttttgg taagccgccg tttataaaca
5341 gggaagatgt cctttgtcaa gggaggcaca gagcatggcc aatttggcaa attgcaggtt
5401 tttctgagtg aaaaatgaaa aagcattgta gtagagtcgg ctcactgaaa aaccggggag
5461 gacgaaaagg tttccagcca cagttgtagt cacgtgcgcg ccatgctgac taatggcagc
5521 cgtcgttggg cagaagagaa ttagtatggt acaggatacg ctaattgcgc tccaactacc
5581 aaggttgttg agggaacact ggggcaatag gctgtcgcca ttcaagagca gattaagtcc
5641 tcagcgagct cgcatggaat gcgtgcgatg agcgacctca tgctatacct gagaaagcaa
5701 cctgacctac aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc
5761 actttaaaat ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa
5821 tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctac
//