LOCUS pCfB2226\(X-4-loxP-SpHIS5syn) 5864 bp DNA circular 1-APR-2014 DEFINITION PCR product of p2070 (pX-4_no_BsaI). KEYWORDS PCR_PRODUCT. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|692965812| COMMENT VNTDBDATE|694086164| COMMENT LSOWNER| COMMENT VNTNAME|pCfB2226 (X-4-loxP-SpHIS5syn)| COMMENT VNTAUTHORNAME|Irina Borodina| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT (CWidget 2 (1 #3# 2) 1 2 0 0 #114# 0 100) COMMENT (LOGPEN 0 8 10053171) 8 1 0.829079 1.9 COMMENT 0.082322 1) COMMENT #186=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #144# 1701 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 700 255 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.833333 255 COMMENT "{\\i Not}I (5090)" "@N (@S)" 5 -12.6246 COMMENT 8.71165 0 -10 6.86018 1.46528 #185#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #187=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1702195557 100) COMMENT (CObjectList) (CObjectList)) COMMENT #188=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1702050409 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 87688576 COMMENT (CStringList) 87672616 87672736 (CObList))) FEATURES Location/Qualifiers CDS complement(3793..4651) /vntifkey="4" /label=AmpR rep_origin 3047..3690 /vntifkey="33" /label=pUC\ori misc_feature 1797..2247 /vntifkey="21" /label=X-4\Down terminator 30..219 /vntifkey="43" /label=T\CYC1 terminator complement(5670..5864) /vntifkey="43" /label=T\ADH1 misc_feature 5110..5631 /vntifkey="21" /label=X-4\UP gene complement(3793..4651) /vntifkey="60" misc_feature 267..300 /vntifkey="21" /label=loxP promoter 301..698 /vntifkey="30" /label=P\TEF CDS 699..1352 /gene="HIS5" /codon_start=1 /transl_table=11 /product="imidazoleglycerol-phosphate dehydratase" /protein_id="AAG34536.1" /db_xref="GI:11344900" /vntifkey="4" /label=HIS5 terminator 1353..1614 /vntifkey="43" /label=T\TEF misc_feature 1615..1648 /vntifkey="21" /label=loxP misc_feature 1..29 /vntifkey="21" /label=USER\cassette\(AsiSI/Nb.BsmI) BASE COUNT 1619 a 1323 c 1298 g 1624 t ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag 721 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac 781 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag 841 aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat catgcactgg 901 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc 961 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta 1021 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gctctttcta 1081 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg 1141 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag 1201 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg 1261 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt 1321 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt 1381 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 1441 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 1501 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 1561 gattcgatac taacgccgcc atccagttta aacgagctct cgagaaccct taatataact 1621 tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc 1681 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 1741 tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc attaataaca 1801 ggcatgggaa gattcgcttt ttttttttga attacaatag tatgtctgat gtctgcaaga 1861 agtaacaggc gtgtgcacaa gaatacgtgt gtgtgcgtaa gcgtatgcac tggtggcata 1921 acttatctaa gaagtatata tcactgacat agaaatgtag atatacaggt atttttctcg 1981 ataatcgata aaaatctcgt cgcgctgaac caaacttggt ggttacggag agtttttctc 2041 tcatcattac tgtctttcgc attgatttcc cctttgaccg ataaaatccc ttggattcat 2101 aagattaaac aaagaggtga tcaaagagaa ccctgtgaaa gtttatgttt ataaccgggc 2161 ataaagtgaa ctagacactt tcaagaagcc aaccaaagca tgagtaacga agcttaccag 2221 catgatcata ccgtaaatcc tcaccagatt aagacctcag cgcggccgca aatttaaata 2281 aaatgaagtg aagttcctat actttctaga gaataggaac ttctatagtg agtcgaataa 2341 gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt atagtttgta 2401 ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt 2461 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca 2521 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa 2581 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat 2641 atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc ctaaactccc 2701 cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac gattactcgt 2761 tatcagaacc gcccaggggg cccgagctta agactggccg tcgttttaca acacagaaag 2821 agtttgtaga aacgcaaaaa ggccatccgt caggggcctt ctgcttagtt tgatgcctgg 2881 cagttcccta ctctcgcctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 2941 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3001 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3061 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 3301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3481 gttcttgaag tggtgggcta actacggcta cactagaaga acagtatttg gtatctgcgc 3541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgcg 3721 cgtaactcac gttaagggat tttggtcatg agcttgcgcc gtcccgtcaa gtcagcgtaa 3781 tgctctgctt ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 3841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 3901 catctggccc cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat 3961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 4021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4081 gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta 4141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 4321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 4381 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 4441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 4561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 4621 taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca 4681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 4741 aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta 4801 tacctgaata tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca 4861 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact 4921 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4981 ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt 5041 tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga 5101 gggtttaatc ccaaagctaa gagtcccatt ttattcttct atatgtatat tttcgatact 5161 ctaaaccacc ctacaatgta gccctatact aaatctgctc aattttcagc ttctacaagt 5221 gactcgaggc cacgtggaaa gatccaacta ctccagcaca acgattcaat ataatcgatt 5281 gctccactca taagaggcaa gaacaagctt caacttttgg taagccgccg tttataaaca 5341 gggaagatgt cctttgtcaa gggaggcaca gagcatggcc aatttggcaa attgcaggtt 5401 tttctgagtg aaaaatgaaa aagcattgta gtagagtcgg ctcactgaaa aaccggggag 5461 gacgaaaagg tttccagcca cagttgtagt cacgtgcgcg ccatgctgac taatggcagc 5521 cgtcgttggg cagaagagaa ttagtatggt acaggatacg ctaattgcgc tccaactacc 5581 aaggttgttg agggaacact ggggcaatag gctgtcgcca ttcaagagca gattaagtcc 5641 tcagcgagct cgcatggaat gcgtgcgatg agcgacctca tgctatacct gagaaagcaa 5701 cctgacctac aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc 5761 actttaaaat ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa 5821 tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctac //