LOCUS Exported 6997 bp ds-DNA circular SYN 01-APR-2014 DEFINITION PCR product of p0384 pXI-2-URA-DR. ACCESSION . VERSION . KEYWORDS pCfB2190 (XI-2-loxP-KlLEU2syn) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6997) AUTHORS Irina Borodina TITLE Direct Submission JOURNAL Exported 17. jun 2015 from SnapGene 2.6.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6997 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..29 /note="USER cassette (AsiSI/Nb.BsmI)" terminator 30..219 /note="T CYC1" misc_feature 251..310 /note="loxP" terminator complement(311..453) /note="T LEU2" CDS complement(454..1542) /gene="LEU2" /note="KL.LEU2" promoter complement(1543..2572) /note="P LEU2" misc_feature 2573..2626 /note="loxP" misc_feature 2771..3333 /note="XI-2 Down" rep_origin complement(4133..4776) /direction=LEFT /note="pUC ori" CDS complement(4879..5737) /note="AmpR" gene complement(4879..5737) /note="Gene_1" misc_feature 6196..6764 /note="XI-2 UP" terminator complement(6803..6997) /note="T ADH1" ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatccgcagg ctaaccggaa cctgtattat ttagtttatg ctacgttaaa 361 taaagacctt tcgttcacat aactgaatgt gtaatggcct tgagatttca agcataccaa 421 gttggtggag acggggtcgt tacaaaagac tctttaagcc aagatttcct tgacagcctt 481 ggcgatagca tcgccaacct cagtggtaga gttagaacca ccaaggtcac cggttctgac 541 acctgcatcc aagacatttc taacagcttc ttcaagagcc ctaccttctt caaccaaatc 601 caaggataac ttcaacatca tagctgcaga taagatggta gcaattgggt taaccttgtt 661 tgctggtaaa tctggggcag aaccatgaca tggttcgtac aaaccgaatg ccttgttagt 721 gtcaggtagg gaagctagag atgcagaagg taataaaccc aaagaacctg gaataacaga 781 ggcttcatcg gagataatat caccaaacat gttgttggta ataacaacac cgtttagctt 841 agttggtgat ttaaccaaaa tcatagcagc agagtcgatc aattggtgct gaacagttaa 901 ttgtgggaac tcagtcttga tggtttcttc aacagtcttt ctccacaatc tggaagaggc 961 aagcacgtta gccttgtcaa gtgaccagat tggtaatggt gggttttgtt gcaatgccaa 1021 gaaagcagcc attcttgtaa ttctttgaac ttcaggaaca ctgtatttct cagagtccca 1081 agcaactccg tcaccttcat cttcttttct ttcaccaaag tagataccac caaccaattc 1141 tctaacgacg acgaaatcgg taccctttgc atattcaggc ttcaaaggag aaagatctag 1201 taaagaatca gaagcaaagt tacatggtct taagttggcg tatagaccca attcctttct 1261 gatcttcaat agaccttgtt ctggtctaac tgcgcccgta ccccattttg gaccaccaac 1321 agcacctagt aagacagcat cggctttctt agaggcttct agagcttcat ctggtaaagg 1381 agtgccagtg gcatcgatgg cagcaccccc gatcaagtga tgttggaaat tgaacttaat 1441 ttctggacgg acttcagcaa tggcattcaa gaccttaata gcttcgtcag taacttcttt 1501 accgacgtga tcacccggta ggacaacgat attcttagac atggtataat ctgtgtagtg 1561 tgggatactt tttacttctt caaataggta tcaacaataa aaataaatcg aagcaaatgt 1621 aggaatgcgt taaagcagat gtacttactt agagtacata gatatattta tataattcaa 1681 tatataaaag tatatgaagc atctgatgtt gaacctgtca tgactctaaa ctggaatggg 1741 tagttatgct tctgaaggtt ttccgttaga aaatgagtgc agggatcagg cccggaaccg 1801 gttttagcct gaaaggaaaa gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa 1861 ttggaatcca ccacaagtac agcgaatggc aagagggaag gggaatatat tgtgcctggc 1921 ggtagtctgc tatatcaaat gttaaacata actaaatgca gtagtgaata gagtaatgta 1981 tttctacata tgtgggtagg gccaatggga gcccgatgtc tattgtacag atattttcct 2041 tttatagtta atcaattcaa cttggaaatc attgagtcga tatctacttg gataactgga 2101 gccgtggcag cgtttgaaat ttgacgagtc tccttgtttt gttcgcgcat tttcagttta 2161 ttcaaacggg agatggcacc gaatccactg acttctctgt tcaatttgaa tggtaatagg 2221 aagaaagtac atttcgagaa attttcgccc aacgtcgaaa tctcatggat gatatcctcg 2281 atacaaatga caccttcatc acctaatttc tcttcaacga tattgttgtc attcaagatt 2341 atctcttttg gttctttatc ttctggcctt tgccacatta ttctagatct cttttggatt 2401 aaagaacgga tagatgcaag tgaaggttgt ccgataacaa tatatggtga tacaagtttc 2461 aacaatgggt acacgtcctt ggtaagtttg atgaaaacac cagtgttcaa ctcaacaagt 2521 ctcaaaagag ataacacttt gtaagccttt gctgggatct tcacagcgag ctctcgagaa 2581 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtgat atcagatcca 2641 ctagtggcct atgcacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 2701 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccctgcagg actagtgctg 2761 aggcattaat cacacgacta gcgctttcag atattaaaaa gtttagatgt aggttttagc 2821 ggtaacagtt atataaatcg tgtttcttct cttgatgaaa caaaaaaatg ctagaaaaac 2881 tttgtcgttt cttacttttg gtgcgctttg cagttttcgt ggctagactt agaatcattt 2941 ctcctcagat ttcttgatta aagtttggtg cgaagcccta ctctaacatt ggtgttcttc 3001 ttttcattca cgcaagttaa gtccaggaag gtgagcaaat gctcatcctt ctgttcatgc 3061 gtgacggctg aattatcctt atctggcgta cccgtgcagc cgtttccgtg cctcggttcc 3121 tccgagatat ccttagggac cgccagggac catgattgcg tcaactgttg tcaccgctcc 3181 agaggatcct ctgtaacctt ttcaaccata aaaatagaaa gggcacaggg ttggggtatt 3241 tgaatttttt ttttgggttt tttcgtatta cttattactt accttctttt ctatataatt 3301 ttgtttttcc ctggtagagc ggaatcttcc cacattaaga cctcagcgcg gccgcaaatt 3361 taaataaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc 3421 gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag 3481 tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt 3541 ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta 3601 tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa 3661 aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc 3721 tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa 3781 actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt 3841 actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac 3901 agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat 3961 gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt 4021 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 4081 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 4141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 4201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 4261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 4321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 4381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 4441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 4501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 4561 tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat 4621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 4801 cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca 4861 gcgtaatgct ctgcttttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4981 gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg 5041 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5101 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5161 ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt 5221 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 5281 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 5341 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5401 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5461 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5521 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5581 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5641 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5701 agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt 5761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5821 ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc 5881 catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg 5941 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg 6001 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc 6061 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag 6121 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg 6181 cgctgagggt ttaatgagga ttttcgatgg agcaggatga ggagaaatag taccacatgt 6241 atatatccat tacaaaaagg tttatataca attacaatag acccttgttg gggtttctga 6301 aaaaagaagt agtcgatgcc atcggcaata atacggaatt acgagaaaca caatcccgat 6361 ccttttttgg gtaattactt caccgattct accgatttat catgccaaaa aaaattcacc 6421 gtgggttcta gaagtgccct ttgaggattg tagccactct aacccacacg gcctccttac 6481 tagctgacta aggtgacaaa accgcaagga ctggaaagtc gccactcatc tgaaaattct 6541 caagtttttc actactgagt ttatgctttc gaattttttt gttcggtaat agcacggcgg 6601 ttcgattcaa ttccgccgct ccgagcgatg ctccgcaaaa ctcagtaata agctttctga 6661 tggttcaccc cttttttagc acgcggggtg taactcaaca gaaaaatgtg ccatagaaca 6721 agactaggca aaagccaagg agcgtttgcc atgaacttcc acaaattaag tcctcagcga 6781 gctcgcatgg aatgcgtgcg atgagcgacc tcatgctata cctgagaaag caacctgacc 6841 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa 6901 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag 6961 aaattcgctt atttagaagt gtcaacaacg tatctac //