LOCUS pCfB2227\(XI-3-loxP-SpHIS5syn) 6002 bp DNA circular 1-APR-2014 DEFINITION PCR product of p2118 (pXI-3_no_PacI_NheI). KEYWORDS PCR_PRODUCT. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|692976233| COMMENT VNTDBDATE|694086183| COMMENT LSOWNER| COMMENT VNTNAME|pCfB2227 (XI-3-loxP-SpHIS5syn)| COMMENT VNTAUTHORNAME|Irina Borodina| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCfB2227 (XI-3-loxP-SpHIS5syn)" 0 6002 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray 21 4) (CWordArray) COMMENT (CStringList "BbsI" "BsaI" "NheI" "NotI" "PacI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 6002 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "BbsI" "gaagac" 9 13 0 0 "") COMMENT #1=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 0 0 "") COMMENT #2=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") COMMENT #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 2349 0 5175 0 0 "") COMMENT #4=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "")) COMMENT (CObList COMMENT #5=(CFSignal (CObList) "AmpR" 4 0 1 3878 4736 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1..859)") COMMENT #6=(CFSignal (CObList) "pUC ori" 33 0 0 3132 3775 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(5388..6031)") COMMENT #7=(CFSignal (CObList) "XI-3 Down" 21 0 0 1797 2332 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4053..4588") COMMENT #8=(CFSignal (CObList) "T CYC1" 43 0 0 30 219 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2155..2344") COMMENT #9=(CFSignal (CObList) "T ADH1" 43 0 1 5808 6002 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1931..2125)") COMMENT #10=(CFSignal (CObList) "MCS 1" 20 0 0 5783 5794 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1906..1917") COMMENT #11=(CFSignal (CObList) "XI-3 UP" 21 0 0 5195 5769 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1318..1892") COMMENT #12=(CFSignal (CObList) "loxP" 21 0 0 267 300 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "41..74") COMMENT #13=(CFSignal (CObList) "P TEF" 30 0 0 301 698 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "75..472") COMMENT #14=(CFSignal (CObList) "HIS5" 4 0 0 699 1352 0 (CStringList) COMMENT (CStringList "/gene=\"HIS5\"" "/codon_start=1" "/transl_table=11" COMMENT "/product=\"imidazoleglycerol-phosphate dehydratase\"" COMMENT "/protein_id=\"AAG34536.1\"" "/db_xref=\"GI:11344900\"") 1 1 1 1 COMMENT "473..1126") COMMENT #15=(CFSignal (CObList) "T TEF" 43 0 0 1353 1614 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1127..1388") COMMENT #16=(CFSignal (CObList) "loxP" 21 0 0 1615 1648 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1389..1422") COMMENT #17=(CFSignal (CObList) "USER cassette (AsiSI/Nb.BsmI)" 21 0 0 1 29 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #18=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #19=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "pCfB2227 (XI-3-loxP-SpHIS5syn)" 2) 5 "" 0 4) COMMENT #20=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pCfB2227 (XI-3-loxP-SpHIS5syn)'" 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "PCR product of p2118 (pXI-3_no_PacI_NheI)" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 03/20/14 01:23" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 04/01/14 09:43" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6002 bp" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #29=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Keywords: PCR_PRODUCT" 1))) "Standard Fields") COMMENT #31=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #35=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 699 (3 #14# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #36=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 699 End: 1352" 1) COMMENT #37=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 473..1126" 1) COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"HIS5\"" 1) COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /codon_start=1" 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /transl_table=11" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /product=\"imidazoleglycerol-phosphate dehydratase\"" COMMENT 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /protein_id=AAG34536.1" COMMENT 1) COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /db_xref=GI:11344900" COMMENT 1))) "HIS5") COMMENT #46=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3878 (3 #5# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3878 End: 4736 (Complementary)" COMMENT 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1..859)" 1))) "AmpR"))) COMMENT "CDS (2 total)") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 20 (7 20 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #51=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5783 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5783 End: 5794" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Oriinal location: 1329..1371" 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1906..1917" 1))) "MCS 1"))) COMMENT "Misc. Binding Site (1 total)") COMMENT #56=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 29 " 1))) COMMENT "USER cassette (AsiSI/Nb.BsmI)") COMMENT #59=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 267 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 267 End: 300 " 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 41..74" 1))) "loxP") COMMENT #63=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1615 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1615 End: 1648" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1389..1422" 1))) "loxP") COMMENT #67=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1797 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1797 End: 2332" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4053..4588" 1))) "XI-3 Down") COMMENT #71=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5195 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5195 End: 5769" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1318..1892" 1))) "XI-3 UP"))) COMMENT "Misc. Feature (5 total)") COMMENT #75=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 301 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 301 End: 698 " 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 75..472" 1))) "P TEF"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #80=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #81=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3132 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3132 End: 3775" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(5388..6031)" 1))) COMMENT "pUC ori"))) "Replication Origin (1 total)") COMMENT #85=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 30 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 30 End: 219 " 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2155..2344" 1))) "T CYC1") COMMENT #90=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1353 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1353 End: 1614" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1127..1388" 1))) "T TEF") COMMENT #94=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5808 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5808 End: 6002 (Complementary)" COMMENT 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1931..2125)" 1))) COMMENT "T ADH1"))) "Terminator (3 total)"))) COMMENT "Feature Map") COMMENT #98=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #99=(CFolderPar COMMENT (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #100=(CFolderPar COMMENT (CGroupPar (CParagraph 3878 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3878 End: 4736 (Complementary)" COMMENT 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1..859)" 1))) "Gene_1"))) COMMENT "Gene (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #104=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #105=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 87245376 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BbsI: 0 sites") 8 12 COMMENT "GAAGACNNNNNNN" "CTTCTGNNNNNNN") COMMENT #106=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 87256640 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BsaI: 0 sites") 7 11 COMMENT "GGTCTCNNNNNN" "CCAGAGNNNNNN") COMMENT #107=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 87235552 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" COMMENT "CGATCG") COMMENT #108=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 87253376 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #109=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 2349 " 1) COMMENT #111=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 5175 " 1))))) "NotI: 2 sites") COMMENT 2 6 "GCGGCCGC" "CGCCGGCG") COMMENT #112=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 87253120 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" COMMENT "AATTAATT"))) "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #113=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #114=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #115=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #116=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #117=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #118=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #119=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #120=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #121=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #122=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #123=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #124=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #125=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #126=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #127=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #128=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #129=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #130=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #131=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #132=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #133=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #134=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #135=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #136=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #137=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #138=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #139=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #140=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #141=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #142=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #143=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #144=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #145=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #146=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #147=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #148=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #149=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #150=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #151=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #152=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 68 COMMENT (CShapeMapEntry 0 "RSite" 1 "Label 3") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 60.0567 60.0567 168.129 COMMENT 168.129 0.1 -6002) 1 1 0 1 1 COMMENT (mapper: 17.9934 -15.6658 60.0567 60.0567 0.01 10 -1 6002 6002 1 0 0) COMMENT #153=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1937009781 100) COMMENT (CObjectList COMMENT #154=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -566 100) COMMENT (CObjectList COMMENT #155=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #119# 85799924 0) COMMENT (LOGPEN 0 2 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27523 6.27323 1 0.0214037) 0.104518) COMMENT #156=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #116# 63638240 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pCfB2227 (XI-3-loxP-SpHIS5syn)" COMMENT "@N" 1 0 0.871165 0 -10 18.0996 1.46528 Nil) COMMENT #157=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #115# 63633912 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "6002 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.18033 0.932452 Nil)) (CObjectList)) COMMENT #158=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1508 100) COMMENT (CObjectList COMMENT #159=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -973 100) COMMENT (CObjectList COMMENT #160=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 1.3232 2.22101 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #161=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63639180 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -13.215 -1.74233 0 -10 2.6808 1.11561 #160#) COMMENT #162=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #117# 1585 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 4.8601 5.54365 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #163=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63639388 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "HIS5" "@N" 1 COMMENT 11.9848 5.22699 0 -10 2.03141 1.11561 #162#)) COMMENT (CObjectList)) COMMENT #164=(CGroupWidget COMMENT (CWidget 20 (7 20 0) 1 2 0 0 Nil -450 100) COMMENT (CObjectList COMMENT #165=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #120# 1316 100) COMMENT (LOGPEN 0 8 6723840) 8 1 0.22994 1.9 0.082322 COMMENT 1) COMMENT #166=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63628024 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "MCS 1" "@N" 1 COMMENT -4.8806 12.1963 0 -10 2.7474 1.11561 #165#)) COMMENT (CObjectList)) COMMENT #167=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -241 100) COMMENT (CObjectList COMMENT #168=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.83582 4.39604 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #169=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63641824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "XI-3 Down" "@N" COMMENT 1 12.4218 -6.96932 0 -10 4.32924 1.11561 COMMENT #168#) COMMENT #170=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #118# 819 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.243528 COMMENT 0.844507 1 0.082322) 0.8 1.8 0) COMMENT #171=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63641404 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "XI-3 UP" "@N" 1 COMMENT -8.39768 10.454 0 -10 3.14703 1.11561 #170#) COMMENT #172=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #122# 86296992 COMMENT 100) (LOGPEN 0 12 13395507) 10 1 5.99517 1.9 COMMENT 0.082322 1) COMMENT #173=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63640032 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT 7.45792 10.454 0 -10 1.91486 1.11561 #172#) COMMENT #174=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #122# 86823232 COMMENT 100) (LOGPEN 0 12 13395507) 10 1 4.58626 1.9 COMMENT 0.082322 1) COMMENT #175=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63628920 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT 12.8814 -1.74233 0 -10 1.91486 1.11561 #174#) COMMENT #176=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #122# 85964928 COMMENT 100) (LOGPEN 0 12 13395507) 10 1 6.27319 1.9 COMMENT 0.082322 1) COMMENT #177=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63642048 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "USER cassette (AsiSI/Nb.BsmI)" "@N" 1 COMMENT 7.54386 13.9386 0 -10 12.9711 1.11561 #176#)) COMMENT (CObjectList)) COMMENT #178=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -136 100) COMMENT (CObjectList COMMENT #179=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #130# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 5.54365 5.95963 1 0.082322) 0.8 1.8 0) COMMENT #180=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63640240 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "P TEF" "@N" 1 COMMENT 9.99107 8.71165 0 -10 2.56424 1.11561 #179#)) COMMENT (CObjectList)) COMMENT #181=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -136 100) COMMENT (CObjectList COMMENT #182=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 2.32762 3.00072 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #183=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63553888 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pUC ori" "@N" 1 COMMENT -8.12872 -10.454 0 -10 3.26358 1.11561 #182#)) COMMENT (CObjectList)) COMMENT #184=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -1601 100) COMMENT (CObjectList COMMENT #185=(CLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #122# 571466689 COMMENT 100) (LOGPEN 0 12 13395507) 10 0.835356 COMMENT 6.04429 6.24287) COMMENT #186=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63628408 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T CYC1" "@N" 1 COMMENT 4.0266 12.1963 0 -10 3.21363 1.11561 #185#) COMMENT #187=(CLine COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #122# 2082537730 COMMENT 100) (LOGPEN 0 12 13395507) 10 0.835356 COMMENT 6.27319 0.203811) COMMENT #188=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63631320 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T ADH1" "@N" 1 COMMENT -3.66943 13.9386 0 -10 3.26358 1.11561 #187#) COMMENT #189=(CLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #122# 0 100) COMMENT (LOGPEN 0 12 13395507) 10 0.835356 4.58626 COMMENT 4.8601) COMMENT #190=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 63639180 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T TEF" "@N" 1 COMMENT 13.2563 0 0 -10 2.51429 1.11561 #189#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #191=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1078 100) COMMENT (CObjectList COMMENT #192=(CGroupWidget COMMENT (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -346 100) COMMENT (CObjectList COMMENT #193=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #121# 86294552 COMMENT 100) (LOGPEN 0 8 10053171) 8 1 3.8191 1.9 COMMENT 0.082322 1) COMMENT #194=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #151# 63617584 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 700 255 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.833333 255 COMMENT "{\\i Not}I (2349)" "@N (@S)" 5 12.0122 COMMENT -8.71165 0 -10 6.86018 1.46528 #193#) COMMENT #195=(CScratch COMMENT (CWidget 2 (1 #3# 2) 1 2 0 0 #121# 86822616 COMMENT 100) (LOGPEN 0 8 10053171) 8 1 0.865411 1.9 COMMENT 0.082322 1) COMMENT #196=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 700 255 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.833333 255 COMMENT "{\\i Not}I (5175)" "@N (@S)" 5 -13.3854 COMMENT 6.96932 0 -10 6.86018 1.46528 #195#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #197=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -346 100) COMMENT (CObjectList) (CObjectList)) COMMENT #198=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1392 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 87688576 COMMENT (CStringList) 87672616 87672736 (CObList))) FEATURES Location/Qualifiers CDS complement(3878..4736) /vntifkey="4" /label=AmpR rep_origin 3132..3775 /vntifkey="33" /label=pUC\ori misc_feature 1797..2332 /vntifkey="21" /label=XI-3\Down terminator 30..219 /vntifkey="43" /label=T\CYC1 terminator complement(5808..6002) /vntifkey="43" /label=T\ADH1 misc_binding 5783..5794 /vntifkey="20" /label=MCS\1 /note="Oriinal location: 1329..1371" misc_feature 5195..5769 /vntifkey="21" /label=XI-3\UP gene complement(3878..4736) /vntifkey="60" misc_feature 267..300 /vntifkey="21" /label=loxP promoter 301..698 /vntifkey="30" /label=P\TEF CDS 699..1352 /gene="HIS5" /codon_start=1 /transl_table=11 /product="imidazoleglycerol-phosphate dehydratase" /protein_id="AAG34536.1" /db_xref="GI:11344900" /vntifkey="4" /label=HIS5 terminator 1353..1614 /vntifkey="43" /label=T\TEF misc_feature 1615..1648 /vntifkey="21" /label=loxP misc_feature 1..29 /vntifkey="21" /label=USER\cassette\(AsiSI/Nb.BsmI) BASE COUNT 1661 a 1348 c 1259 g 1734 t ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag 721 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac 781 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag 841 aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat catgcactgg 901 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc 961 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta 1021 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gctctttcta 1081 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg 1141 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag 1201 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg 1261 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt 1321 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt 1381 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 1441 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 1501 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 1561 gattcgatac taacgccgcc atccagttta aacgagctct cgagaaccct taatataact 1621 tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc 1681 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 1741 tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc attaatttac 1801 gtggattgag ccagcaatac agatcattat taaactgttt tgtacatgat gttagtatat 1861 aatcgtaaag cttttctaat atgtatacct tatacatgga actccacaga acttgcaaac 1921 ataccaaaaa tcctttattc ttgttcactc attttacatc aaaaaataat atttcagtta 1981 ttaaggaaaa taaaaaaata gattagagaa gcattttgaa gaaatagtat attcttttat 2041 tgaacctaag agcgtgatat ttttactcga aataaaatac gaaaaatcta tacactcatc 2101 tttccgacta ctattggctc ctgctcaaaa aaagagggaa aaaaagctcc aaaattctat 2161 cttttcctat cgctcctgtc ctatccttat tacgttcatt actattttaa tactatccat 2221 tcttttattt tcagtctaaa aaaaacattt ctcataacgg gaaaagcaaa aaaatgtcaa 2281 gcttatacat caaaacacca ctgcatgcat tatctgctgg tccggattct caattaagac 2341 ctcagcgcgg ccgcaaattt aaataaaatg aagtgaagtt cctatacttt ctagagaata 2401 ggaacttcta tagtgagtcg aataagggcg acacaaaatt tattctaaat gcataataaa 2461 tactgataac atcttatagt ttgtattata ttttgtatta tcgttgacat gtataatttt 2521 gatatcaaaa actgattttc cctttattat tttcgagatt tattttctta attctcttta 2581 acaaactaga aatattgtat atacaaaaaa tcataaataa tagatgaata gtttaattat 2641 aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat 2701 ttataaggtt aaataattct catatatcaa gcaaagtgac aggcgccctt aaatattctg 2761 acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt 2821 tatttgcgga ttaacgatta ctcgttatca gaaccgccca gggggcccga gcttaagact 2881 ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg 2941 gccttctgct tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca 3001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta 3601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3781 ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt 3841 gcgccgtccc gtcaagtcag cgtaatgctc tgcttttacc aatgcttaat cagtgaggca 3901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 3961 ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat accgcgagaa 4021 ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag ggccgagcgc 4081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 4141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc tacaggcatc 4201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 4261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 4321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 4381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 4441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 4501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 4561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 4621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 4681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatattc 4741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4801 tttgaatgta tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa ttaaccaatt 4861 ctgaacatta tcgcgagccc atttatacct gaatatggct cataacaccc cttgtttgcc 4921 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg 4981 tagcgccgat ggtagtgtgg ggactcccca tgcgagagta gggaactgcc aggcatcaaa 5041 taaaacgaaa ggctcagtcg aaagactggg cctttcgccc gggctaatta tggggtgtcg 5101 cccttattcg actctatagt gaagttccta ttctctagaa agtataggaa cttctgaagt 5161 ggggatttaa atgcggccgc gctgagggtt taatagttac ttgctctatg cgtttgcgca 5221 tcctcttttt actttttttt tttcagtaaa gcctaagcat aaatcgtttt atacgtacga 5281 cacgttcaac ttttcttggt tagtagtggc aatctctgca atacatacag ggagtcatgg 5341 tctatcatct tgtccaatca aagaagcatc ggttcagatc gagcaaactg tagggagaaa 5401 ggaaagtaga aatgcagagt gtgctatatg tccaatctcg gttttgtagt ttggatgtca 5461 ttagagatct accacccaac cggctgcttt catgtggaac agaaaagaaa tcggggcgct 5521 tcctcttctg tattccttta gttaacgttt ttattcagcc atctaaccat cataccccca 5581 tacggtaaca aaacctcttc taagaaaaga agtctctgct cctccgccat cttattttta 5641 ttcgctgcgc gcgtttattg tcgcatcgct ggccagcaaa aaattggttg ccttttttta 5701 cctaaaaaag acacatctaa ctgattagtt ttccgtttta ggatattgac gccaagcgtg 5761 cgtctgatta ttaagtcctc agcgagctcg catggaatgc gtgcgatgag cgacctcatg 5821 ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata agaattttcg 5881 ttttaaaacc taagagtcac tttaaaattt gtatacactt atttttttta taacttattt 5941 aataataaaa atcataaatc ataagaaatt cgcttattta gaagtgtcaa caacgtatct 6001 ac //