LOCUS       pCfB2229\(XI-5-loxP-CaLYS5syn)        6233 bp    DNA     circular      1-APR-2014
DEFINITION  PCR product of p0387 (pXI-5-URA3-DR).
KEYWORDS    PCR_PRODUCT.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|692976636|
COMMENT     VNTDBDATE|694086334|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2229 (XI-5-loxP-CaLYS5syn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
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FEATURES             Location/Qualifiers
     rep_origin      3275..3918
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    1965..2475
                     /vntifkey="21"
                     /label=XI-5\Down
     terminator      30..219
                     /vntifkey="43"
                     /label=CYC1
     terminator      complement(6039..6233)
                     /vntifkey="43"
                     /label=ADH1
     misc_feature    5338..6000
                     /vntifkey="21"
                     /label=XI-5\UP
     gene            complement(4021..4879)
                     /vntifkey="60"
     misc_feature    267..300
                     /vntifkey="21"
                     /label=loxP
     terminator      1518..1782
                     /vntifkey="43"
                     /label=T\TEF
     misc_feature    1783..1816
                     /vntifkey="21"
                     /label=loxP
     promoter        301..698
                     /vntifkey="30"
                     /label=P\TEF
     CDS             699..1517
                     /vntifkey="4"
                     /label=LYS5
     misc_feature    complement(4019..4879)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1744 a      1415 c      1314 g      1760 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggttaaaacg actgaagtag 
      721 taagcgaagt ttcaaaggtg gcaggtgtaa gaccatgggc aggtatattc gttgttgaaa 
      781 ttcaagagga tatactcgcg gatgagttta cgttcgaggc attaatgaga actttgccat 
      841 tggcgtctca agccagaatc ctcaataaaa aatcgtttca cgatagatgt tcaaatctat 
      901 gcagccagct gctgcagttg tttggctgct ctatagtaac gggcttaaat tttcaagagc 
      961 tgaaatttga caagggcagc ttcggtaagc cattcttaga caacaatcgt tttcttccat 
     1021 ttagcatgac catcggtgaa caatatgtag ctatgttcct cgtaaaatgt gtaagtacag 
     1081 atgaatacca ggatgtcgga attgatatcg cttctccgtg caattatggc gggagggaag 
     1141 agttggagct atttaaagaa gtttttagtg aaagagaatt taacggttta ctgaaagcgt 
     1201 ctgatccatg cacaatattt acttacttat ggtccttgaa ggagtcgtat acaaaattta 
     1261 ctggaactgg ccttaacaca gacttgtcac taatagattt tggcgctatc agcttttttc 
     1321 cggctgaggg agcttctatg tgcataactc tggatgaagt tccattgatt ttccattctc 
     1381 aatggttcaa taacgaaatt gtcactatct gtatgccaaa gtccatcagt gataaaatca 
     1441 acacgaacag accaaaatta tataatatca gcttatctac gttgattgat tatttcatcg 
     1501 aaaatgatgg tttataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc 
     1561 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta 
     1621 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat 
     1681 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg 
     1741 ccgccatcca gtgtcgaaaa cgagctctcg agaaccctta atataacttc gtataatgta 
     1801 tgctatacga agttattagg tgatatcaga tccactagtg gcctatgcac ccaattcgcc 
     1861 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 
     1921 aaaccctggc gttacccctg caggactagt gctgaggcat taatgccttc gatttgacac 
     1981 atctctaagc tgaaactgag aatactgttg taaaacaggt attggctgct tcatagtaca 
     2041 cccaattgcc cacaacccaa gttaatcata agctcttgtt gtcgatgaat tgctcaaaat 
     2101 gtggccattc tttcaatttt gcacctctaa aactgctccg tgggttgtga caacaacctt 
     2161 aagggtaatg gactttttta gaaagacaaa tgagatgatt tcacctactg gtatatcatt 
     2221 tatttataca cgaccgtttt gacatttcag agttgcacgt tggtatatcc taatattctc 
     2281 acaagcatca cagtgaagaa acgcaagaaa atggaaaaaa ttcccaggtg gttgttgttt 
     2341 tctcttatat cgtcggtgct gtgcatactt ggggccctgt gtgtgccgtt gttatcggtt 
     2401 gccttcgata gcaagcgcaa tagccaatct aagttggtca actatggtct ttctctaagt 
     2461 gccggatcta tgatcattaa gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa 
     2521 gttcctatac tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa 
     2581 atttattcta aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta 
     2641 ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag 
     2701 atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa 
     2761 taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt 
     2821 aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat caagcaaagt 
     2881 gacaggcgcc cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa 
     2941 cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc 
     3001 ccagggggcc cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa 
     3061 cgcaaaaagg ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact 
     3121 ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     3181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     3241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     3301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     3361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     3421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     3481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     3541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     3601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     3661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     3721 gtgggctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 
     3781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     3841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     3901 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt 
     3961 taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctttt 
     4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     4081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4141 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
     4201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     4261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     4321 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     4381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     4441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     4501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     4561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     4621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     4681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     4741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     4801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     4861 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
     4981 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     5041 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     5101 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     5161 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     5221 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     5281 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgcg 
     5341 gagaagtcgt tgatagcatt tccgaaggct tttccacatt gactgcagta tctaaaagag 
     5401 ctccagcagc ggtcttagta gttcctgtgc ttgccgcttt gcagtataca gggacattgg 
     5461 gaactacgga ggaaaaatcg gaatgctctg agctcttctc aaaaccagac gaactcgtct 
     5521 tatttctcag taaattaccc aacatataaa ctctccctct tcaccttctc tacaaggcca 
     5581 cacacaagac agttttaaca ggcaaatggt actggaggct ctcaccaatg gggtttaaga 
     5641 cgctccaaac acacgctctc ctttttttct ttatatatcg gtagcaacga aagctagtcg 
     5701 cagaacgtta cacactaaag cagatcaatc aatcaatcct gagaacctgc ttcgttcaaa 
     5761 ccatccacta gaagaaacac gcacgtgtac ccaaaaatag agacaaacaa acagacaaaa 
     5821 ataacaatga ttcctaccga ctaaagtaaa acaacactca cgtaataaaa aatcctagat 
     5881 acaactcact tgtattcctc tcttctttga ccatcactaa agcttctctc ttactatatt 
     5941 cctcaaataa aaaaaagttg caatgattac ttaccaatgt gccataaact ccgtgcacca 
     6001 attaagtcct cagcgagctc gcatggaatg cgtgcgatga gcgacctcat gctatacctg 
     6061 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac 
     6121 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa 
     6181 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc tac 
//
