LOCUS       pCfB2336\(XII-5-loxP-SpHIS5syn)        6021 bp    DNA     circular      1-APR-2014
DEFINITION  PCR product of p2073 (pXII-5_no_BsaI).
KEYWORDS    PCR_PRODUCT.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|692976995|
COMMENT     VNTDBDATE|694086402|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2336 (XII-5-loxP-SpHIS5syn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "PCR product of p2073 (pXII-5_no_BsaI)" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(4016..4876)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    5335..5788
                     /vntifkey="21"
                     /label=XII-5\UP
     terminator      5829..5990
                     /vntifkey="43"
                     /label=T\ADH1
     terminator      30..218
                     /vntifkey="43"
                     /label=T\CYC1
     misc_feature    1797..2472
                     /vntifkey="21"
                     /label=XII-5\Down
     rep_origin      3249..3914
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    267..300
                     /vntifkey="21"
                     /label=loxP
     promoter        301..698
                     /vntifkey="30"
                     /label=P\TEF
     CDS             699..1352
                     /gene="HIS5"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="AAG34536.1"
                     /db_xref="GI:11344900"
                     /vntifkey="4"
                     /label=HIS5
     terminator      1353..1614
                     /vntifkey="43"
                     /label=T\TEF
     misc_feature    1615..1648
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1635 a      1329 c      1323 g      1734 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaggagg gcttttgtag 
      721 aaagaaatac gaacgaaacg aaaatcagcg ttgccatcgc tttggacaaa gctcccttac 
      781 ctgaagagtc gaattttatt gatgaactta taacttccaa gcatgcaaac caaaagggag 
      841 aacaagtaat ccaagtagac acgggaattg gattcttgga tcacatgtat catgcactgg 
      901 ctaaacatgc aggctggagc ttacgacttt actcaagagg tgatttaatc atcgatgatc 
      961 atcacactgc agaagatact gctattgcac ttggtattgc attcaagcag gctatgggta 
     1021 actttgccgg cgttaaaaga tttggacatg cttattgtcc acttgacgaa gctctttcta 
     1081 gaagcgtagt tgacttgtcg ggacggccct atgctgttat cgatttggga ttaaagcgtg 
     1141 aaaaggttgg ggaattgtcc tgtgaaatga tccctcactt actatattcc ttttcggtag 
     1201 cagctggaat tactttgcat gttacctgct tatatggtag taatgaccat catcgtgctg 
     1261 aaagcgcttt taaatctctg gctgttgcca tgcgcgcggc tactagtctt actggaagtt 
     1321 ctgaagtccc aagcacgaag ggagtgttgt aaagagtact gacaataaaa agattcttgt 
     1381 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 
     1441 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 
     1501 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 
     1561 gattcgatac taacgccgcc atccagttta aacgagctct cgagaaccct taatataact 
     1621 tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag tggcctatgc 
     1681 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     1741 tcgtgactgg gaaaaccctg gcgttacccc tgcaggacta gtgctgaggc attaatgcaa 
     1801 ctcagaagtt tgacagcaag caagttcatc attcgaacta gccttattgt tttagttcag 
     1861 tgacagcgaa ctgccgtact cgatgcttta tttctcacgg tagagcggaa gaacagatag 
     1921 gggcagcgtg agaagagtta gaaagtaaat ttttatcacg tctgaagtat tcttattcat 
     1981 aggaaatttt gcaaggtttt ttagctcaat aacgggctaa gttatataag gtgttcacgc 
     2041 gattttcttg ttatgtatac ctcttctctg aggaatggta ctactgtcct gatgtaggct 
     2101 ccttaaattg gtgggcaaga ataacttatc gatattttgt atattggtct tggagttcac 
     2161 cacgtaatgc ctgtttaaga ccatcagtta actctagtat tatttggtct tggctactgg 
     2221 ccgtttgcta ttattcaagt cttttgtgcc ttcccgtcgg gtaagggagt tatttaggga 
     2281 tacagaatct aacgaaaact aaatctcaat gattaactct atttaatcct tttttgaaag 
     2341 gcaaaagagg tcccttgttc acttacaacg ttcttagcca aattcgctta tcacttacta 
     2401 cttcacgata tacagaagta aaaacatata aaaagatgtc tgtttgttta gccatcacaa 
     2461 aaggtatcgc agattaagac ctcagcgcgg ccgcaaattt aaataaaatg aagtgaagtt 
     2521 cctatacttt ctagagaata ggaacttcta tagtgagtcg aataagggcg acacaaaatt 
     2581 tattctaaat gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta 
     2641 tcgttgacat gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt 
     2701 tattttctta attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa 
     2761 tagatgaata gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa 
     2821 gtgcgttgct tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac 
     2881 aggcgccctt aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc 
     2941 gccgaagcgg gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca 
     3001 gggggcccga gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc 
     3061 aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc 
     3121 gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     3241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3721 ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     3781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     3841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     3901 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa 
     3961 gggattttgg tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgcttttacc 
     4021 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     4081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg 
     4141 ctgcgatgat accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc 
     4201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     4261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     4321 ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     4381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     4441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     4501 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     4561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     4621 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     4681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     4741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     4801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     4861 aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat cagggttatt 
     4921 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg 
     4981 ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct 
     5041 cataacaccc cttgtttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg 
     5101 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca tgcgagagta 
     5161 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgccc 
     5221 gggctaatta tggggtgtcg cccttattcg actctatagt gaagttccta ttctctagaa 
     5281 agtataggaa cttctgaagt ggggatttaa atgcggccgc gctgagggtt taatcaatct 
     5341 ggcggcttga gttctcaaca tgttttattt tttacttata ttgctggtag ggtaaaaaaa 
     5401 tataactcct aggaataggt tgtctatatg tttttgtctt gcttctataa ttgtaacaaa 
     5461 caaggaaagg gaaaatactg ggtgtaaaag ccattgagtc aagttaggtc atccctttta 
     5521 tacaaaattt ttcaattttt tttccaagat tcttgtacga ttaattattt tttttttgcg 
     5581 tcctacagcg tgatgaaaat ttcgcctgct gcaagatgag cgggaacggg cgaaatgtgc 
     5641 acgcgcacaa cttacgaaac gcggatgagt cactgacagc caccgcagag gttctgactc 
     5701 ctactgagct ctattggagg tggcagaacc ggtaccggag gaggccgcta taaccggttt 
     5761 gaatttattg tcacagtgtc acatcagcat taagtcctca gcgagctcgc atggaatgcg 
     5821 tgcgatgagc gacctcatgc tatacctgag aaagcaacct gacctacagg aaagagttac 
     5881 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta 
     5941 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag 
     6001 aagtgtcaac aacgtatcta c 
//
