LOCUS pCfB2193\(X-2-loxP-NatMXsyn) 5834 bp DNA circular UNA 1-APR-2014
DEFINITION .
ACCESSION BGHA
VERSION .
KEYWORDS p0126 pX-2-natMXsyn.
SOURCE Saccharomyces cerevisiae.
ORGANISM Saccharomyces cerevisiae
REFERENCE 1 (bases 1 to 5834)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 25. feb 2014 from SnapGene 2.2.2
http://www.snapgene.com
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|691784323|
COMMENT VNTDBDATE|694115732|
COMMENT LSOWNER|
COMMENT VNTNAME|pCfB2193 (X-2-loxP-NatMXsyn)|
COMMENT VNTAUTHORNAME|Irina Borodina|
COMMENT VNTOAUTHORNAME|UNKNOWN|
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FEATURES Location/Qualifiers
source 3754..3753
/organism="Saccharomyces cerevisiae"
/mol_type="genomic DNA"
/vntifkey="98"
source 227..1602
/organism="unspecified"
/mol_type="genomic DNA"
/vntifkey="98"
gene complement(3754..4612)
/vntifkey="60"
CDS complement(3754..4612)
/codon_start=1
/vntifkey="4"
/label=AmpR
/note="AmpR"
misc_feature 5071..5601
/vntifkey="21"
/label=X-2\UP
/note="X-2 UP"
terminator 5640..5834
/vntifkey="43"
/label=T\ADH1
/note="T ADH1"
terminator 30..219
/vntifkey="43"
/label=T\CYC1
/note="T CYC1"
CDS 267..300
/gene="loxP"
/product="recognition site for Cre recombinase"
/vntifkey="4"
/label=loxP
/note="loxP"
promoter 301..697
/gene="TEF1p"
/product="TEF1 promoter of A. gossypii"
/vntifkey="29"
/label=TEF1p
CDS 699..1271
/gene="nat"
/product="Nourseothricin acetyltransferase"
/vntifkey="4"
/label=nat
/note="nat"
terminator 1272..1536
/gene="TEF1t"
/product="TEF1 terminator of A. gossypii"
/vntifkey="43"
/label=TEF1t
CDS 1537..1570
/gene="loxP"
/product="recognition site for Cre recombinase"
/vntifkey="4"
/label=loxP
/note="loxP"
misc_feature 1719..2208
/vntifkey="21"
/label=X-2\Down
/note="X-2 Down"
rep_origin 3008..3651
/direction=LEFT
/vntifkey="33"
/label=pUC\ori
/note="pUC ori"
misc_feature 1..29
/vntifkey="21"
/label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT 1493 a 1419 c 1329 g 1593 t
ORIGIN
1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga
61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg
241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca
721 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg
781 ggtccttcac caccgacacc gttttccgcg tcaccgccac cggggacggc ttcaccctgc
841 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg
901 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg
961 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg
1021 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
1081 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca
1141 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg
1201 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc
1261 cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt
1321 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt
1381 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg
1441 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca
1501 tccagtgtcg aaaacgagct ctcgagaacc cttaatataa cttcgtataa tgtatgctat
1561 acgaagttat taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag
1621 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
1681 tggcgttacc cctgcaggac tagtgctgag gcattaatcc tgcataatcg gcctcacaga
1741 gggatcccgt tacccatcta tgctgaagat ttatcatact attcctccgc tcgtttcttt
1801 tttcagtgag gtgtgtcgtg aaagaaaacc cacaattaaa ctttcaacaa ccgggcgact
1861 aggaagagag tagtgggcgc ggatgacgaa ggctaaggtc acttcttcgt ttcctttatt
1921 ggggtttccg tgtagccttc ccctgaatag tgtgggacgt tttatgagaa gccgtaagaa
1981 ataggcaaat tgagttatga caagtagaca tgatgccgca gccttgcctg actttacgtc
2041 tccttcatga ataagttttt ctatcgagtt cttttccttt tttcgcctta attagctcaa
2101 ttaagcctgt cctcactact tttctttttc ttatcggctt tgtgccacac ctaaccttcg
2161 aatgctgttt tattccgttc ttacatggga tggtaatgcc ttggcgagat taagacctca
2221 gcgcggccgc aaatttaaat aaaatgaagt gaagttccta tactttctag agaataggaa
2281 cttctatagt gagtcgaata agggcgacac aaaatttatt ctaaatgcat aataaatact
2341 gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata
2401 tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa
2461 actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt
2521 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat
2581 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat attctgacaa
2641 atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt
2701 tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt aagactggcc
2761 gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg tcaggggcct
2821 tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct cgctcactga
2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3421 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag
3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
3661 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagcttgcgc
3721 cgtcccgtca agtcagcgta atgctctgct tttaccaatg cttaatcagt gaggcaccta
3781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3841 ctacgatacg ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac
3901 gctcaccggc tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
4021 taagtagttc gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg
4081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
4141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
4201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
4261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
4321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
4381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
4441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
4501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
4561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc
4621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4681 aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga
4741 acattatcgc gagcccattt atacctgaat atggctcata acaccccttg tttgcctggc
4801 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
4861 gccgatggta gtgtggggac tccccatgcg agagtaggga actgccaggc atcaaataaa
4921 acgaaaggct cagtcgaaag actgggcctt tcgcccgggc taattatggg gtgtcgccct
4981 tattcgactc tatagtgaag ttcctattct ctagaaagta taggaacttc tgaagtgggg
5041 atttaaatgc ggccgcgctg agggtttaat cgtctatgag gagactgtta gttggatatc
5101 agtaatgaga cgaaaaagct cgaaatgaat ggatatattc tttttgctac tggcaactgt
5161 tgaatattta atgttaaaac aaactaactg aggtatattc gtatctgtat gtacacatat
5221 actatataca ggaaaagata agcaagagag aggatatcaa ctacgagagc gatcgattat
5281 atatcaaaag ctgtccgctt tgccacccat aatcggcgct tagtttcgga gttcaatcat
5341 aattctacca ccttacactc aacttactct ttaactccta tagtataata tcgccactga
5401 ccccatatta aaaaattttt ttgctcgatc ttctatcctc tttaggttaa ttgtcgctgt
5461 tattgtctag attttttctc ggagatggcg catctatttg ccgtcaaaag atcctctcat
5521 accatattaa gtaaattgcc tccatttctt tttcctcggg cagagaaact cgcaggcaac
5581 ttgctctcga agtggtcacg tattaagtcc tcagcgagct cgcatggaat gcgtgcgatg
5641 agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
5701 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
5761 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc
5821 aacaacgtat ctac
//