LOCUS pCfB2193\(X-2-loxP-NatMXsyn) 5834 bp DNA circular UNA 1-APR-2014 DEFINITION . ACCESSION BGHA VERSION . KEYWORDS p0126 pX-2-natMXsyn. SOURCE Saccharomyces cerevisiae. ORGANISM Saccharomyces cerevisiae REFERENCE 1 (bases 1 to 5834) AUTHORS . TITLE Direct Submission JOURNAL Exported 25. feb 2014 from SnapGene 2.2.2 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|691784323| COMMENT VNTDBDATE|694115732| COMMENT LSOWNER| COMMENT VNTNAME|pCfB2193 (X-2-loxP-NatMXsyn)| COMMENT VNTAUTHORNAME|Irina Borodina| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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2147483648 268697654 (CObList))) FEATURES Location/Qualifiers source 3754..3753 /organism="Saccharomyces cerevisiae" /mol_type="genomic DNA" /vntifkey="98" source 227..1602 /organism="unspecified" /mol_type="genomic DNA" /vntifkey="98" gene complement(3754..4612) /vntifkey="60" CDS complement(3754..4612) /codon_start=1 /vntifkey="4" /label=AmpR /note="AmpR" misc_feature 5071..5601 /vntifkey="21" /label=X-2\UP /note="X-2 UP" terminator 5640..5834 /vntifkey="43" /label=T\ADH1 /note="T ADH1" terminator 30..219 /vntifkey="43" /label=T\CYC1 /note="T CYC1" CDS 267..300 /gene="loxP" /product="recognition site for Cre recombinase" /vntifkey="4" /label=loxP /note="loxP" promoter 301..697 /gene="TEF1p" /product="TEF1 promoter of A. gossypii" /vntifkey="29" /label=TEF1p CDS 699..1271 /gene="nat" /product="Nourseothricin acetyltransferase" /vntifkey="4" /label=nat /note="nat" terminator 1272..1536 /gene="TEF1t" /product="TEF1 terminator of A. gossypii" /vntifkey="43" /label=TEF1t CDS 1537..1570 /gene="loxP" /product="recognition site for Cre recombinase" /vntifkey="4" /label=loxP /note="loxP" misc_feature 1719..2208 /vntifkey="21" /label=X-2\Down /note="X-2 Down" rep_origin 3008..3651 /direction=LEFT /vntifkey="33" /label=pUC\ori /note="pUC ori" misc_feature 1..29 /vntifkey="21" /label=USER\cassette\(AsiSI/Nb.BsmI) BASE COUNT 1493 a 1419 c 1329 g 1593 t ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca 721 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg 781 ggtccttcac caccgacacc gttttccgcg tcaccgccac cggggacggc ttcaccctgc 841 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg 901 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg 961 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg 1021 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg 1081 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca 1141 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg 1201 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc 1261 cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt 1321 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt 1381 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg 1441 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca 1501 tccagtgtcg aaaacgagct ctcgagaacc cttaatataa cttcgtataa tgtatgctat 1561 acgaagttat taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag 1621 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 1681 tggcgttacc cctgcaggac tagtgctgag gcattaatcc tgcataatcg gcctcacaga 1741 gggatcccgt tacccatcta tgctgaagat ttatcatact attcctccgc tcgtttcttt 1801 tttcagtgag gtgtgtcgtg aaagaaaacc cacaattaaa ctttcaacaa ccgggcgact 1861 aggaagagag tagtgggcgc ggatgacgaa ggctaaggtc acttcttcgt ttcctttatt 1921 ggggtttccg tgtagccttc ccctgaatag tgtgggacgt tttatgagaa gccgtaagaa 1981 ataggcaaat tgagttatga caagtagaca tgatgccgca gccttgcctg actttacgtc 2041 tccttcatga ataagttttt ctatcgagtt cttttccttt tttcgcctta attagctcaa 2101 ttaagcctgt cctcactact tttctttttc ttatcggctt tgtgccacac ctaaccttcg 2161 aatgctgttt tattccgttc ttacatggga tggtaatgcc ttggcgagat taagacctca 2221 gcgcggccgc aaatttaaat aaaatgaagt gaagttccta tactttctag agaataggaa 2281 cttctatagt gagtcgaata agggcgacac aaaatttatt ctaaatgcat aataaatact 2341 gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata 2401 tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa 2461 actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt 2521 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat 2581 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat attctgacaa 2641 atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt 2701 tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt aagactggcc 2761 gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg tcaggggcct 2821 tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct cgctcactga 2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3421 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag 3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3661 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagcttgcgc 3721 cgtcccgtca agtcagcgta atgctctgct tttaccaatg cttaatcagt gaggcaccta 3781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 3841 ctacgatacg ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac 3901 gctcaccggc tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 3961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 4021 taagtagttc gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg 4081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 4141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 4201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 4261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 4321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 4381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 4441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 4501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 4561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc 4621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 4681 aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga 4741 acattatcgc gagcccattt atacctgaat atggctcata acaccccttg tttgcctggc 4801 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc 4861 gccgatggta gtgtggggac tccccatgcg agagtaggga actgccaggc atcaaataaa 4921 acgaaaggct cagtcgaaag actgggcctt tcgcccgggc taattatggg gtgtcgccct 4981 tattcgactc tatagtgaag ttcctattct ctagaaagta taggaacttc tgaagtgggg 5041 atttaaatgc ggccgcgctg agggtttaat cgtctatgag gagactgtta gttggatatc 5101 agtaatgaga cgaaaaagct cgaaatgaat ggatatattc tttttgctac tggcaactgt 5161 tgaatattta atgttaaaac aaactaactg aggtatattc gtatctgtat gtacacatat 5221 actatataca ggaaaagata agcaagagag aggatatcaa ctacgagagc gatcgattat 5281 atatcaaaag ctgtccgctt tgccacccat aatcggcgct tagtttcgga gttcaatcat 5341 aattctacca ccttacactc aacttactct ttaactccta tagtataata tcgccactga 5401 ccccatatta aaaaattttt ttgctcgatc ttctatcctc tttaggttaa ttgtcgctgt 5461 tattgtctag attttttctc ggagatggcg catctatttg ccgtcaaaag atcctctcat 5521 accatattaa gtaaattgcc tccatttctt tttcctcggg cagagaaact cgcaggcaac 5581 ttgctctcga agtggtcacg tattaagtcc tcagcgagct cgcatggaat gcgtgcgatg 5641 agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa 5701 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt 5761 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc 5821 aacaacgtat ctac //