LOCUS       pCfB2513\(X-2-loxP-hphMXsyn)        6290 bp    DNA     circular UNA 21-SEP-2015
DEFINITION  .
ACCESSION   BGHA
VERSION     .
KEYWORDS    p0126 pX-2-hphMXsyn.
SOURCE      Saccharomyces cerevisiae.
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 4. jun 2014 from SnapGene 2.3.3
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|745596534|
COMMENT     VNTDBDATE|745597324|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2513 (X-2-loxP-hphMXsyn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     source          4210..4209
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     source          227..2058
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     CDS             complement(4210..5068)
                     /codon_start=1
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR"
     gene            complement(4210..5068)
                     /vntifkey="60"
     misc_feature    5527..6057
                     /vntifkey="21"
                     /label=X-2\UP
                     /note="X-2 UP"
     terminator      6096..6290
                     /vntifkey="43"
                     /label=T\ADH1
                     /note="T ADH1"
     terminator      30..219
                     /vntifkey="43"
                     /label=T\CYC1
                     /note="T CYC1"
     CDS             267..300
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     misc_feature    320..698
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1p
     CDS             699..1727
                     /gene="hph"
                     /product="hygromycin B resistance"
                     /vntifkey="4"
                     /label=hph
                     /note="hph"
     misc_feature    1728..1967
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1t
     CDS             1993..2026
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     misc_feature    2175..2664
                     /vntifkey="21"
                     /label=X-2\Down
                     /note="X-2 Down"
     rep_origin      complement(3464..4107)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="pUC ori"
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1616 a      1499 c      1441 g      1734 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca 
      721 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 
      781 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 
      841 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact 
      901 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc 
      961 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg 
     1021 aactgcccgc tgttctgcag ccggtcgcgg aggcaatgga tgccattgct gcggccgatc 
     1081 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat 
     1141 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg 
     1201 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg 
     1261 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg 
     1321 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat 
     1381 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc 
     1441 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc 
     1501 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag 
     1561 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta 
     1621 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg 
     1681 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa 
     1741 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt 
     1801 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc 
     1861 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt 
     1921 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg 
     1981 agaaccctta atataacttc gtataatgta tgctatacga agttattagg tgatatcaga 
     2041 tccactagtg gcctatgcac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     2101 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccctg caggactagt 
     2161 gctgaggcat taatcctgca taatcggcct cacagaggga tcccgttacc catctatgct 
     2221 gaagatttat catactattc ctccgctcgt ttcttttttc agtgaggtgt gtcgtgaaag 
     2281 aaaacccaca attaaacttt caacaaccgg gcgactagga agagagtagt gggcgcggat 
     2341 gacgaaggct aaggtcactt cttcgtttcc tttattgggg tttccgtgta gccttcccct 
     2401 gaatagtgtg ggacgtttta tgagaagccg taagaaatag gcaaattgag ttatgacaag 
     2461 tagacatgat gccgcagcct tgcctgactt tacgtctcct tcatgaataa gtttttctat 
     2521 cgagttcttt tccttttttc gccttaatta gctcaattaa gcctgtcctc actacttttc 
     2581 tttttcttat cggctttgtg ccacacctaa ccttcgaatg ctgttttatt ccgttcttac 
     2641 atgggatggt aatgccttgg cgagattaag acctcagcgc ggccgcaaat ttaaataaaa 
     2701 tgaagtgaag ttcctatact ttctagagaa taggaacttc tatagtgagt cgaataaggg 
     2761 cgacacaaaa tttattctaa atgcataata aatactgata acatcttata gtttgtatta 
     2821 tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt 
     2881 attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa 
     2941 aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt 
     3001 atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc 
     3061 aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc 
     3121 ataaaaaaac ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat 
     3181 cagaaccgcc cagggggccc gagcttaaga ctggccgtcg ttttacaaca cagaaagagt 
     3241 ttgtagaaac gcaaaaaggc catccgtcag gggccttctg cttagtttga tgcctggcag 
     3301 ttccctactc tcgccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     3361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     3421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     3481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     3541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     3601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     3661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     3721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     3781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     3841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     3901 cttgaagtgg tgggctaact acggctacac tagaagaaca gtatttggta tctgcgctct 
     3961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     4021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     4081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgcgcgt 
     4141 aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc 
     4201 tctgctttta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4321 ctggccccag cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag 
     4381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4501 tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     4801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     4861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5041 gggcgacacg gaaatgttga atactcatat tcttcctttt tcaatattat tgaagcattt 
     5101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5161 taggggtcag tgttacaacc aattaaccaa ttctgaacat tatcgcgagc ccatttatac 
     5221 ctgaatatgg ctcataacac cccttgtttg cctggcggca gtagcgcggt ggtcccacct 
     5281 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggactccc 
     5341 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg 
     5401 ggcctttcgc ccgggctaat tatggggtgt cgcccttatt cgactctata gtgaagttcc 
     5461 tattctctag aaagtatagg aacttctgaa gtggggattt aaatgcggcc gcgctgaggg 
     5521 tttaatcgtc tatgaggaga ctgttagttg gatatcagta atgagacgaa aaagctcgaa 
     5581 atgaatggat atattctttt tgctactggc aactgttgaa tatttaatgt taaaacaaac 
     5641 taactgaggt atattcgtat ctgtatgtac acatatacta tatacaggaa aagataagca 
     5701 agagagagga tatcaactac gagagcgatc gattatatat caaaagctgt ccgctttgcc 
     5761 acccataatc ggcgcttagt ttcggagttc aatcataatt ctaccacctt acactcaact 
     5821 tactctttaa ctcctatagt ataatatcgc cactgacccc atattaaaaa atttttttgc 
     5881 tcgatcttct atcctcttta ggttaattgt cgctgttatt gtctagattt tttctcggag 
     5941 atggcgcatc tatttgccgt caaaagatcc tctcatacca tattaagtaa attgcctcca 
     6001 tttctttttc ctcgggcaga gaaactcgca ggcaacttgc tctcgaagtg gtcacgtatt 
     6061 aagtcctcag cgagctcgca tggaatgcgt gcgatgagcg acctcatgct atacctgaga 
     6121 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta 
     6181 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat 
     6241 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac 
//
