LOCUS       pCfB2194\(X-4-loxP-HphMXsyn)        6242 bp    DNA     circular UNA  1-APR-2014
DEFINITION  .
ACCESSION   BGHA
VERSION     .
KEYWORDS    p2070 pX-4_no_BsaI-hphMXsyn.
SOURCE      Saccharomyces cerevisiae.
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 7. feb 2014 from SnapGene 2.2.1
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|691784527|
COMMENT     VNTDBDATE|694085909|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2194 (X-4-loxP-HphMXsyn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     source          4171..4170
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     source          227..2058
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     CDS             complement(4171..5029)
                     /codon_start=1
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR"
     gene            complement(4171..5029)
                     /vntifkey="60"
     misc_feature    5488..6009
                     /vntifkey="21"
                     /label=X-4\UP
                     /note="X-4 UP"
     terminator      6048..6242
                     /vntifkey="43"
                     /label=T\ADH1
                     /note="T ADH1"
     terminator      30..219
                     /vntifkey="43"
                     /label=T\CYC1
                     /note="T CYC1"
     CDS             267..300
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     misc_feature    320..698
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1p
     CDS             699..1727
                     /gene="hph"
                     /product="hygromycin B resistance"
                     /vntifkey="4"
                     /label=hph
                     /note="hph"
     misc_feature    1728..1967
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1t
     CDS             1993..2026
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
                     /note="loxP"
     misc_feature    2175..2625
                     /vntifkey="21"
                     /label=X-4\Down
                     /note="X-4 Down"
     rep_origin      3425..4068
                     /direction=LEFT
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="pUC ori"
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1650 a      1479 c      1456 g      1657 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca 
      721 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 
      781 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 
      841 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact 
      901 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc 
      961 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg 
     1021 aactgcccgc tgttctgcag ccggtcgcgg aggcaatgga tgccattgct gcggccgatc 
     1081 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat 
     1141 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg 
     1201 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg 
     1261 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg 
     1321 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat 
     1381 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc 
     1441 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc 
     1501 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag 
     1561 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta 
     1621 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg 
     1681 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa 
     1741 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt 
     1801 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc 
     1861 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt 
     1921 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg 
     1981 agaaccctta atataacttc gtataatgta tgctatacga agttattagg tgatatcaga 
     2041 tccactagtg gcctatgcac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     2101 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccctg caggactagt 
     2161 gctgaggcat taataacagg catgggaaga ttcgcttttt ttttttgaat tacaatagta 
     2221 tgtctgatgt ctgcaagaag taacaggcgt gtgcacaaga atacgtgtgt gtgcgtaagc 
     2281 gtatgcactg gtggcataac ttatctaaga agtatatatc actgacatag aaatgtagat 
     2341 atacaggtat ttttctcgat aatcgataaa aatctcgtcg cgctgaacca aacttggtgg 
     2401 ttacggagag tttttctctc atcattactg tctttcgcat tgatttcccc tttgaccgat 
     2461 aaaatccctt ggattcataa gattaaacaa agaggtgatc aaagagaacc ctgtgaaagt 
     2521 ttatgtttat aaccgggcat aaagtgaact agacactttc aagaagccaa ccaaagcatg 
     2581 agtaacgaag cttaccagca tgatcatacc gtaaatcctc accagattaa gacctcagcg 
     2641 cggccgcaaa tttaaataaa atgaagtgaa gttcctatac tttctagaga ataggaactt 
     2701 ctatagtgag tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat 
     2761 aacatcttat agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca 
     2821 aaaactgatt ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact 
     2881 agaaatattg tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt 
     2941 catcaatcga aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag 
     3001 gttaaataat tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg 
     3061 ctctttccct aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc 
     3121 ggattaacga ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc 
     3181 gttttacaac acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct 
     3241 gcttagtttg atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc 
     3301 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     3361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     3421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     3481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     3541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     3601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     3661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     3721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     3781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     3841 tgtaggcggt gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac 
     3901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     3961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     4021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     4081 tcagtggaac gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt 
     4141 cccgtcaagt cagcgtaatg ctctgctttt accaatgctt aatcagtgag gcacctatct 
     4201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     4261 cgatacggga gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct 
     4321 caccggctcc ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     4381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     4441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt 
     4501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     4561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     4621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     4681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     4741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     4801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     4861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     4921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     4981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ttcttccttt 
     5041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     5101 gtatttagaa aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca 
     5161 ttatcgcgag cccatttata cctgaatatg gctcataaca ccccttgttt gcctggcggc 
     5221 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 
     5281 gatggtagtg tggggactcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 
     5341 aaaggctcag tcgaaagact gggcctttcg cccgggctaa ttatggggtg tcgcccttat 
     5401 tcgactctat agtgaagttc ctattctcta gaaagtatag gaacttctga agtggggatt 
     5461 taaatgcggc cgcgctgagg gtttaatccc aaagctaaga gtcccatttt attcttctat 
     5521 atgtatattt tcgatactct aaaccaccct acaatgtagc cctatactaa atctgctcaa 
     5581 ttttcagctt ctacaagtga ctcgaggcca cgtggaaaga tccaactact ccagcacaac 
     5641 gattcaatat aatcgattgc tccactcata agaggcaaga acaagcttca acttttggta 
     5701 agccgccgtt tataaacagg gaagatgtcc tttgtcaagg gaggcacaga gcatggccaa 
     5761 tttggcaaat tgcaggtttt tctgagtgaa aaatgaaaaa gcattgtagt agagtcggct 
     5821 cactgaaaaa ccggggagga cgaaaaggtt tccagccaca gttgtagtca cgtgcgcgcc 
     5881 atgctgacta atggcagccg tcgttgggca gaagagaatt agtatggtac aggatacgct 
     5941 aattgcgctc caactaccaa ggttgttgag ggaacactgg ggcaataggc tgtcgccatt 
     6001 caagagcaga ttaagtcctc agcgagctcg catggaatgc gtgcgatgag cgacctcatg 
     6061 ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata agaattttcg 
     6121 ttttaaaacc taagagtcac tttaaaattt gtatacactt atttttttta taacttattt 
     6181 aataataaaa atcataaatc ataagaaatt cgcttattta gaagtgtcaa caacgtatct 
     6241 ac 
//
