LOCUS pCfB2196\(XI-5-loxP-BLEsyn) 5795 bp DNA circular UNA 1-APR-2014
DEFINITION .
ACCESSION BGHA
VERSION .
KEYWORDS p387 pXI-5-BLEsyn.
SOURCE Saccharomyces cerevisiae.
ORGANISM Saccharomyces cerevisiae
REFERENCE 1 (bases 1 to 5795)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 7. feb 2014 from SnapGene 2.2.1
http://www.snapgene.com
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
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COMMENT VNTDATE|691785631|
COMMENT VNTDBDATE|694085965|
COMMENT LSOWNER|
COMMENT VNTNAME|pCfB2196 (XI-5-loxP-BLEsyn)|
COMMENT VNTAUTHORNAME|Irina Borodina|
COMMENT VNTOAUTHORNAME|UNKNOWN|
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FEATURES Location/Qualifiers
source 3583..3582
/organism="Saccharomyces cerevisiae"
/mol_type="genomic DNA"
/vntifkey="98"
source 227..1410
/organism="PCR template vector pUG66"
/mol_type="other DNA"
/db_xref="taxon:142963"
/vntifkey="98"
/note="derived from Saccharomyces cerevisiae"
gene complement(3583..4441)
/vntifkey="60"
misc_feature 4900..5562
/vntifkey="21"
/label=XI-5\UP
/note="XI-5 UP"
terminator 5601..5795
/vntifkey="43"
/label=ADH1
/note="ADH1"
terminator 30..219
/vntifkey="43"
/label=CYC1
/note="CYC1"
misc_feature 267..300
/vntifkey="21"
/label=loxP
/note="loxP loxP site"
promoter 301..697
/vntifkey="30"
/label=P\TEF
/note="P TEF TEF2 promoter from A. gossypii"
CDS 699..1079
/codon_start=1
/transl_table=11
/gene="ble"
/product="bleomycin resistance protein"
/db_xref="GI:11344913"
/protein_id="AAG34545.1"
/vntifkey="4"
/label=ble
/note="ble Tn5; select for PhleoR"
gene 699..1079
/gene="ble"
/vntifkey="60"
/note="ble"
terminator 1086..1344
/vntifkey="43"
/label=T\TEF
/note="T TEF TEF2 terminator from A. gossypii"
misc_feature 1345..1378
/vntifkey="21"
/label=loxP
/note="loxP loxP site"
misc_feature 1527..2037
/vntifkey="21"
/label=XI-5\Down
/note="XI-5 Down"
rep_origin 2837..3480
/direction=LEFT
/vntifkey="33"
/label=pUC\ori
/note="pUC ori"
misc_feature 1..29
/vntifkey="21"
/label=USER\cassette\(AsiSI/Nb.BsmI)
CDS complement(3583..4441)
/vntifkey="4"
/label=AmpR
BASE COUNT 1566 a 1396 c 1252 g 1581 t
ORIGIN
1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga
61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg
241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
661 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggccgaccaa gcgacgccca
721 acctgccatc acgagatttc gattccacgg ccgccttcta tgaaaggttg ggcttcggaa
781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcaag ctggagttct
841 tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg
901 acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct
961 atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg
1021 acccggacgg gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgat
1081 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta
1141 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga
1201 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat
1261 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa
1321 aacgagctct cgagaaccct taatataact tcgtataatg tatgctatac gaagttatta
1381 ggtgatatca gatccactag tggcctatgc acccaattcg ccctatagtg agtcgtatta
1441 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttacccc
1501 tgcaggacta gtgctgaggc attaatgcct tcgatttgac acatctctaa gctgaaactg
1561 agaatactgt tgtaaaacag gtattggctg cttcatagta cacccaattg cccacaaccc
1621 aagttaatca taagctcttg ttgtcgatga attgctcaaa atgtggccat tctttcaatt
1681 ttgcacctct aaaactgctc cgtgggttgt gacaacaacc ttaagggtaa tggacttttt
1741 tagaaagaca aatgagatga tttcacctac tggtatatca tttatttata cacgaccgtt
1801 ttgacatttc agagttgcac gttggtatat cctaatattc tcacaagcat cacagtgaag
1861 aaacgcaaga aaatggaaaa aattcccagg tggttgttgt tttctcttat atcgtcggtg
1921 ctgtgcatac ttggggccct gtgtgtgccg ttgttatcgg ttgccttcga tagcaagcgc
1981 aatagccaat ctaagttggt caactatggt ctttctctaa gtgccggatc tatgatcatt
2041 aagacctcag cgcggccgca aatttaaata aaatgaagtg aagttcctat actttctaga
2101 gaataggaac ttctatagtg agtcgaataa gggcgacaca aaatttattc taaatgcata
2161 ataaatactg ataacatctt atagtttgta ttatattttg tattatcgtt gacatgtata
2221 attttgatat caaaaactga ttttcccttt attattttcg agatttattt tcttaattct
2281 ctttaacaaa ctagaaatat tgtatataca aaaaatcata aataatagat gaatagttta
2341 attataggtg ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt
2401 ctcatttata aggttaaata attctcatat atcaagcaaa gtgacaggcg cccttaaata
2461 ttctgacaaa tgctctttcc ctaaactccc cccataaaaa aacccgccga agcgggtttt
2521 tacgttattt gcggattaac gattactcgt tatcagaacc gcccaggggg cccgagctta
2581 agactggccg tcgttttaca acacagaaag agtttgtaga aacgcaaaaa ggccatccgt
2641 caggggcctt ctgcttagtt tgatgcctgg cagttcccta ctctcgcctt ccgcttcctc
2701 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
2761 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
2821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
3181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
3241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtgggcta actacggcta
3301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
3361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
3421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
3481 ggggtctgac gctcagtgga acgacgcgcg cgtaactcac gttaagggat tttggtcatg
3541 agcttgcgcc gtcccgtcaa gtcagcgtaa tgctctgctt ttaccaatgc ttaatcagtg
3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
3661 tgtagataac tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc
3721 gagaaccacg ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg
3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag
3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
4441 tattcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
4501 acatatttga atgtatttag aaaaataaac aaataggggt cagtgttaca accaattaac
4561 caattctgaa cattatcgcg agcccattta tacctgaata tggctcataa caccccttgt
4621 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
4681 cgccgtagcg ccgatggtag tgtggggact ccccatgcga gagtagggaa ctgccaggca
4741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgcccgggct aattatgggg
4801 tgtcgccctt attcgactct atagtgaagt tcctattctc tagaaagtat aggaacttct
4861 gaagtgggga tttaaatgcg gccgcgctga gggtttaatg cggagaagtc gttgatagca
4921 tttccgaagg cttttccaca ttgactgcag tatctaaaag agctccagca gcggtcttag
4981 tagttcctgt gcttgccgct ttgcagtata cagggacatt gggaactacg gaggaaaaat
5041 cggaatgctc tgagctcttc tcaaaaccag acgaactcgt cttatttctc agtaaattac
5101 ccaacatata aactctccct cttcaccttc tctacaaggc cacacacaag acagttttaa
5161 caggcaaatg gtactggagg ctctcaccaa tggggtttaa gacgctccaa acacacgctc
5221 tccttttttt ctttatatat cggtagcaac gaaagctagt cgcagaacgt tacacactaa
5281 agcagatcaa tcaatcaatc ctgagaacct gcttcgttca aaccatccac tagaagaaac
5341 acgcacgtgt acccaaaaat agagacaaac aaacagacaa aaataacaat gattcctacc
5401 gactaaagta aaacaacact cacgtaataa aaaatcctag atacaactca cttgtattcc
5461 tctcttcttt gaccatcact aaagcttctc tcttactata ttcctcaaat aaaaaaaagt
5521 tgcaatgatt acttaccaat gtgccataaa ctccgtgcac caattaagtc ctcagcgagc
5581 tcgcatggaa tgcgtgcgat gagcgacctc atgctatacc tgagaaagca acctgaccta
5641 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa
5701 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa
5761 attcgcttat ttagaagtgt caacaacgta tctac
//