LOCUS pCfB2196\(XI-5-loxP-BLEsyn) 5795 bp DNA circular UNA 1-APR-2014 DEFINITION . ACCESSION BGHA VERSION . KEYWORDS p387 pXI-5-BLEsyn. SOURCE Saccharomyces cerevisiae. ORGANISM Saccharomyces cerevisiae REFERENCE 1 (bases 1 to 5795) AUTHORS . TITLE Direct Submission JOURNAL Exported 7. feb 2014 from SnapGene 2.2.1 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|691785631| COMMENT VNTDBDATE|694085965| COMMENT LSOWNER| COMMENT VNTNAME|pCfB2196 (XI-5-loxP-BLEsyn)| COMMENT VNTAUTHORNAME|Irina Borodina| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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6.86018 1.46528 #231#) COMMENT #233=(CScratch COMMENT (CWidget 2 (1 #3# 2) 1 2 0 0 #162# 1076 100) COMMENT (LOGPEN 0 8 10053171) 8 1 0.991585 1.9 COMMENT 0.082322 1) COMMENT #234=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #192# 981390393 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 700 255 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.833333 255 COMMENT "{\\i Not}I (4880)" "@N (@S)" 5 -13.6689 COMMENT 6.96932 0 -10 6.86018 1.46528 #233#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #235=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -869 100) COMMENT (CObjectList) (CObjectList)) COMMENT #236=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -346 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) COMMENT -2147483648 (CStringList) 2147483648 268697654 (CObList))) FEATURES Location/Qualifiers source 3583..3582 /organism="Saccharomyces cerevisiae" /mol_type="genomic DNA" /vntifkey="98" source 227..1410 /organism="PCR template vector pUG66" /mol_type="other DNA" /db_xref="taxon:142963" /vntifkey="98" /note="derived from Saccharomyces cerevisiae" gene complement(3583..4441) /vntifkey="60" misc_feature 4900..5562 /vntifkey="21" /label=XI-5\UP /note="XI-5 UP" terminator 5601..5795 /vntifkey="43" /label=ADH1 /note="ADH1" terminator 30..219 /vntifkey="43" /label=CYC1 /note="CYC1" misc_feature 267..300 /vntifkey="21" /label=loxP /note="loxP loxP site" promoter 301..697 /vntifkey="30" /label=P\TEF /note="P TEF TEF2 promoter from A. gossypii" CDS 699..1079 /codon_start=1 /transl_table=11 /gene="ble" /product="bleomycin resistance protein" /db_xref="GI:11344913" /protein_id="AAG34545.1" /vntifkey="4" /label=ble /note="ble Tn5; select for PhleoR" gene 699..1079 /gene="ble" /vntifkey="60" /note="ble" terminator 1086..1344 /vntifkey="43" /label=T\TEF /note="T TEF TEF2 terminator from A. gossypii" misc_feature 1345..1378 /vntifkey="21" /label=loxP /note="loxP loxP site" misc_feature 1527..2037 /vntifkey="21" /label=XI-5\Down /note="XI-5 Down" rep_origin 2837..3480 /direction=LEFT /vntifkey="33" /label=pUC\ori /note="pUC ori" misc_feature 1..29 /vntifkey="21" /label=USER\cassette\(AsiSI/Nb.BsmI) CDS complement(3583..4441) /vntifkey="4" /label=AmpR BASE COUNT 1566 a 1396 c 1252 g 1581 t ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 661 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggccgaccaa gcgacgccca 721 acctgccatc acgagatttc gattccacgg ccgccttcta tgaaaggttg ggcttcggaa 781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcaag ctggagttct 841 tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg 901 acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct 961 atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg 1021 acccggacgg gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgat 1081 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta 1141 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga 1201 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat 1261 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa 1321 aacgagctct cgagaaccct taatataact tcgtataatg tatgctatac gaagttatta 1381 ggtgatatca gatccactag tggcctatgc acccaattcg ccctatagtg agtcgtatta 1441 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttacccc 1501 tgcaggacta gtgctgaggc attaatgcct tcgatttgac acatctctaa gctgaaactg 1561 agaatactgt tgtaaaacag gtattggctg cttcatagta cacccaattg cccacaaccc 1621 aagttaatca taagctcttg ttgtcgatga attgctcaaa atgtggccat tctttcaatt 1681 ttgcacctct aaaactgctc cgtgggttgt gacaacaacc ttaagggtaa tggacttttt 1741 tagaaagaca aatgagatga tttcacctac tggtatatca tttatttata cacgaccgtt 1801 ttgacatttc agagttgcac gttggtatat cctaatattc tcacaagcat cacagtgaag 1861 aaacgcaaga aaatggaaaa aattcccagg tggttgttgt tttctcttat atcgtcggtg 1921 ctgtgcatac ttggggccct gtgtgtgccg ttgttatcgg ttgccttcga tagcaagcgc 1981 aatagccaat ctaagttggt caactatggt ctttctctaa gtgccggatc tatgatcatt 2041 aagacctcag cgcggccgca aatttaaata aaatgaagtg aagttcctat actttctaga 2101 gaataggaac ttctatagtg agtcgaataa gggcgacaca aaatttattc taaatgcata 2161 ataaatactg ataacatctt atagtttgta ttatattttg tattatcgtt gacatgtata 2221 attttgatat caaaaactga ttttcccttt attattttcg agatttattt tcttaattct 2281 ctttaacaaa ctagaaatat tgtatataca aaaaatcata aataatagat gaatagttta 2341 attataggtg ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt 2401 ctcatttata aggttaaata attctcatat atcaagcaaa gtgacaggcg cccttaaata 2461 ttctgacaaa tgctctttcc ctaaactccc cccataaaaa aacccgccga agcgggtttt 2521 tacgttattt gcggattaac gattactcgt tatcagaacc gcccaggggg cccgagctta 2581 agactggccg tcgttttaca acacagaaag agtttgtaga aacgcaaaaa ggccatccgt 2641 caggggcctt ctgcttagtt tgatgcctgg cagttcccta ctctcgcctt ccgcttcctc 2701 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 2761 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 2821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtgggcta actacggcta 3301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3481 ggggtctgac gctcagtgga acgacgcgcg cgtaactcac gttaagggat tttggtcatg 3541 agcttgcgcc gtcccgtcaa gtcagcgtaa tgctctgctt ttaccaatgc ttaatcagtg 3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 3661 tgtagataac tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc 3721 gagaaccacg ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg 3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag 3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 4441 tattcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 4501 acatatttga atgtatttag aaaaataaac aaataggggt cagtgttaca accaattaac 4561 caattctgaa cattatcgcg agcccattta tacctgaata tggctcataa caccccttgt 4621 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa 4681 cgccgtagcg ccgatggtag tgtggggact ccccatgcga gagtagggaa ctgccaggca 4741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgcccgggct aattatgggg 4801 tgtcgccctt attcgactct atagtgaagt tcctattctc tagaaagtat aggaacttct 4861 gaagtgggga tttaaatgcg gccgcgctga gggtttaatg cggagaagtc gttgatagca 4921 tttccgaagg cttttccaca ttgactgcag tatctaaaag agctccagca gcggtcttag 4981 tagttcctgt gcttgccgct ttgcagtata cagggacatt gggaactacg gaggaaaaat 5041 cggaatgctc tgagctcttc tcaaaaccag acgaactcgt cttatttctc agtaaattac 5101 ccaacatata aactctccct cttcaccttc tctacaaggc cacacacaag acagttttaa 5161 caggcaaatg gtactggagg ctctcaccaa tggggtttaa gacgctccaa acacacgctc 5221 tccttttttt ctttatatat cggtagcaac gaaagctagt cgcagaacgt tacacactaa 5281 agcagatcaa tcaatcaatc ctgagaacct gcttcgttca aaccatccac tagaagaaac 5341 acgcacgtgt acccaaaaat agagacaaac aaacagacaa aaataacaat gattcctacc 5401 gactaaagta aaacaacact cacgtaataa aaaatcctag atacaactca cttgtattcc 5461 tctcttcttt gaccatcact aaagcttctc tcttactata ttcctcaaat aaaaaaaagt 5521 tgcaatgatt acttaccaat gtgccataaa ctccgtgcac caattaagtc ctcagcgagc 5581 tcgcatggaa tgcgtgcgat gagcgacctc atgctatacc tgagaaagca acctgaccta 5641 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa 5701 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa 5761 attcgcttat ttagaagtgt caacaacgta tctac //