LOCUS       pCfB2196\(XI-5-loxP-BLEsyn)        5795 bp    DNA     circular UNA  1-APR-2014
DEFINITION  .
ACCESSION   BGHA
VERSION     .
KEYWORDS    p387 pXI-5-BLEsyn.
SOURCE      Saccharomyces cerevisiae.
  ORGANISM  Saccharomyces cerevisiae
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 7. feb 2014 from SnapGene 2.2.1
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|691785631|
COMMENT     VNTDBDATE|694085965|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2196 (XI-5-loxP-BLEsyn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     source          3583..3582
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     source          227..1410
                     /organism="PCR template vector pUG66"
                     /mol_type="other DNA"
                     /db_xref="taxon:142963"
                     /vntifkey="98"
                     /note="derived from Saccharomyces cerevisiae"
     gene            complement(3583..4441)
                     /vntifkey="60"
     misc_feature    4900..5562
                     /vntifkey="21"
                     /label=XI-5\UP
                     /note="XI-5 UP"
     terminator      5601..5795
                     /vntifkey="43"
                     /label=ADH1
                     /note="ADH1"
     terminator      30..219
                     /vntifkey="43"
                     /label=CYC1
                     /note="CYC1"
     misc_feature    267..300
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP loxP site"
     promoter        301..697
                     /vntifkey="30"
                     /label=P\TEF
                     /note="P TEF TEF2 promoter from A. gossypii"
     CDS             699..1079
                     /codon_start=1
                     /transl_table=11
                     /gene="ble"
                     /product="bleomycin resistance protein"
                     /db_xref="GI:11344913"
                     /protein_id="AAG34545.1"
                     /vntifkey="4"
                     /label=ble
                     /note="ble Tn5; select for PhleoR"
     gene            699..1079
                     /gene="ble"
                     /vntifkey="60"
                     /note="ble"
     terminator      1086..1344
                     /vntifkey="43"
                     /label=T\TEF
                     /note="T TEF TEF2 terminator from A. gossypii"
     misc_feature    1345..1378
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP loxP site"
     misc_feature    1527..2037
                     /vntifkey="21"
                     /label=XI-5\Down
                     /note="XI-5 Down"
     rep_origin      2837..3480
                     /direction=LEFT
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="pUC ori"
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
     CDS             complement(3583..4441)
                     /vntifkey="4"
                     /label=AmpR
BASE COUNT     1566 a      1396 c      1252 g      1581 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggccgaccaa gcgacgccca 
      721 acctgccatc acgagatttc gattccacgg ccgccttcta tgaaaggttg ggcttcggaa 
      781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcaag ctggagttct 
      841 tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg 
      901 acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct 
      961 atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg 
     1021 acccggacgg gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgat 
     1081 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta 
     1141 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga 
     1201 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat 
     1261 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa 
     1321 aacgagctct cgagaaccct taatataact tcgtataatg tatgctatac gaagttatta 
     1381 ggtgatatca gatccactag tggcctatgc acccaattcg ccctatagtg agtcgtatta 
     1441 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttacccc 
     1501 tgcaggacta gtgctgaggc attaatgcct tcgatttgac acatctctaa gctgaaactg 
     1561 agaatactgt tgtaaaacag gtattggctg cttcatagta cacccaattg cccacaaccc 
     1621 aagttaatca taagctcttg ttgtcgatga attgctcaaa atgtggccat tctttcaatt 
     1681 ttgcacctct aaaactgctc cgtgggttgt gacaacaacc ttaagggtaa tggacttttt 
     1741 tagaaagaca aatgagatga tttcacctac tggtatatca tttatttata cacgaccgtt 
     1801 ttgacatttc agagttgcac gttggtatat cctaatattc tcacaagcat cacagtgaag 
     1861 aaacgcaaga aaatggaaaa aattcccagg tggttgttgt tttctcttat atcgtcggtg 
     1921 ctgtgcatac ttggggccct gtgtgtgccg ttgttatcgg ttgccttcga tagcaagcgc 
     1981 aatagccaat ctaagttggt caactatggt ctttctctaa gtgccggatc tatgatcatt 
     2041 aagacctcag cgcggccgca aatttaaata aaatgaagtg aagttcctat actttctaga 
     2101 gaataggaac ttctatagtg agtcgaataa gggcgacaca aaatttattc taaatgcata 
     2161 ataaatactg ataacatctt atagtttgta ttatattttg tattatcgtt gacatgtata 
     2221 attttgatat caaaaactga ttttcccttt attattttcg agatttattt tcttaattct 
     2281 ctttaacaaa ctagaaatat tgtatataca aaaaatcata aataatagat gaatagttta 
     2341 attataggtg ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt 
     2401 ctcatttata aggttaaata attctcatat atcaagcaaa gtgacaggcg cccttaaata 
     2461 ttctgacaaa tgctctttcc ctaaactccc cccataaaaa aacccgccga agcgggtttt 
     2521 tacgttattt gcggattaac gattactcgt tatcagaacc gcccaggggg cccgagctta 
     2581 agactggccg tcgttttaca acacagaaag agtttgtaga aacgcaaaaa ggccatccgt 
     2641 caggggcctt ctgcttagtt tgatgcctgg cagttcccta ctctcgcctt ccgcttcctc 
     2701 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2761 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     2821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     3001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     3061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     3181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     3241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtgggcta actacggcta 
     3301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     3361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     3421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     3481 ggggtctgac gctcagtgga acgacgcgcg cgtaactcac gttaagggat tttggtcatg 
     3541 agcttgcgcc gtcccgtcaa gtcagcgtaa tgctctgctt ttaccaatgc ttaatcagtg 
     3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     3661 tgtagataac tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc 
     3721 gagaaccacg ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg 
     3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag 
     3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     4441 tattcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     4501 acatatttga atgtatttag aaaaataaac aaataggggt cagtgttaca accaattaac 
     4561 caattctgaa cattatcgcg agcccattta tacctgaata tggctcataa caccccttgt 
     4621 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa 
     4681 cgccgtagcg ccgatggtag tgtggggact ccccatgcga gagtagggaa ctgccaggca 
     4741 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgcccgggct aattatgggg 
     4801 tgtcgccctt attcgactct atagtgaagt tcctattctc tagaaagtat aggaacttct 
     4861 gaagtgggga tttaaatgcg gccgcgctga gggtttaatg cggagaagtc gttgatagca 
     4921 tttccgaagg cttttccaca ttgactgcag tatctaaaag agctccagca gcggtcttag 
     4981 tagttcctgt gcttgccgct ttgcagtata cagggacatt gggaactacg gaggaaaaat 
     5041 cggaatgctc tgagctcttc tcaaaaccag acgaactcgt cttatttctc agtaaattac 
     5101 ccaacatata aactctccct cttcaccttc tctacaaggc cacacacaag acagttttaa 
     5161 caggcaaatg gtactggagg ctctcaccaa tggggtttaa gacgctccaa acacacgctc 
     5221 tccttttttt ctttatatat cggtagcaac gaaagctagt cgcagaacgt tacacactaa 
     5281 agcagatcaa tcaatcaatc ctgagaacct gcttcgttca aaccatccac tagaagaaac 
     5341 acgcacgtgt acccaaaaat agagacaaac aaacagacaa aaataacaat gattcctacc 
     5401 gactaaagta aaacaacact cacgtaataa aaaatcctag atacaactca cttgtattcc 
     5461 tctcttcttt gaccatcact aaagcttctc tcttactata ttcctcaaat aaaaaaaagt 
     5521 tgcaatgatt acttaccaat gtgccataaa ctccgtgcac caattaagtc ctcagcgagc 
     5581 tcgcatggaa tgcgtgcgat gagcgacctc atgctatacc tgagaaagca acctgaccta 
     5641 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa 
     5701 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa 
     5761 attcgcttat ttagaagtgt caacaacgta tctac 
//
