LOCUS pCfB2225\(pXII-2-loxP-KanMXsyn) 6144 bp DNA circular SYN 21-SEP-2015 DEFINITION . ACCESSION . VERSION . KEYWORDS p0120 pXII-2-kanMXsyn. SOURCE synthetic DNA construct. ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6144) AUTHORS . TITLE Direct Submission JOURNAL Exported 7. feb 2014 from SnapGene 2.2.1 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|745597978| COMMENT VNTDBDATE|745598047| COMMENT LSOWNER| COMMENT VNTNAME|pCfB2225 (pXII-2-loxP-KanMXsyn)| COMMENT VNTAUTHORNAME|Irina Borodina| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT 100) (LOGPEN 0 8 10053171) 8 1 0.804569 1.9 COMMENT 0.082322 1) COMMENT #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #209# 1 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 87 33 0 0 700 255 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.833333 255 COMMENT "{\\i Not}I (5357)" "@N (@S)" 5 -12.4237 COMMENT 8.71165 0 -10 6.86018 1.46528 #250#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #252=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -136 100) COMMENT (CObjectList) (CObjectList)) COMMENT #253=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -811 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 10 2081718712 (CObList))) FEATURES Location/Qualifiers source 3995..3994 /organism="synthetic DNA construct" /mol_type="other DNA" /vntifkey="98" source 227..1839 /organism="PCR template vector pUG6" /mol_type="other DNA" /db_xref="taxon:142962" /vntifkey="98" /note="derived from Saccharomyces cerevisiae" source 1107..1130 /organism="PCR template vector pUG6" /mol_type="other DNA" /db_xref="taxon:142962" /vntifkey="98" /note="derived from Saccharomyces cerevisiae" CDS complement(4058..4918) /codon_start=1 /vntifkey="4" /label=AmpR /note="AmpR" misc_feature 5377..5911 /vntifkey="21" /label=XII-2\UP /note="XII-2 UP" terminator 5952..6112 /vntifkey="43" /label=T\ADH1 /note="T ADH1" terminator 30..218 /vntifkey="43" /label=T\CYC1 /note="T CYC1" misc_feature complement(267..300) /vntifkey="21" /label=loxP /note="loxP loxP;loxP site" promoter 301..698 /vntifkey="30" /label=P\TEF /note="P TEF P TEF;TEF2 promoter from Ashbya gossypii" gene 699..1508 /gene="kanMX" /vntifkey="60" /note="kanMX" CDS 699..1508 /codon_start=1 /transl_table=11 /gene="kanMX" /product="geneticin resistance protein" /db_xref="GI:11344910" /protein_id="AAG34543.1" /vntifkey="4" /label=kanMX /note="kanMX kanMX;Tn903; selection phenotype G418R" gene 1107..1130 /gene="kanMX" /vntifkey="60" /note="kanMX" terminator 1509..1773 /vntifkey="43" /label=T\TEF /note="T TEF T TEF;TEF2terminator from Ashbya gossypii" misc_feature complement(1774..1807) /vntifkey="21" /label=loxP /note="loxP loxP;loxP site" misc_feature 1956..2514 /vntifkey="21" /label=XII-2\Down /note="XII-2 Down" rep_origin 3291..3956 /direction=RIGHT /vntifkey="33" /label=pUC\ori /note="pUC ori" misc_feature 1..29 /vntifkey="21" /label=USER\cassette\(AsiSI/Nb.BsmI) BASE COUNT 1665 a 1387 c 1320 g 1772 t ORIGIN 1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg 721 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 781 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 841 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 901 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 961 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat 1021 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 1081 ggttacattc gattcctgtt tgtaattgtc cttttaacag cgaccgtgta tttcgtctcg 1141 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 1201 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac 1261 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 1321 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 1381 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 1441 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 1501 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata 1561 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt 1621 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt 1681 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc 1741 agtgtcgaaa acgagctctc gagaaccctt aatataactt cgtataatgt atgctatacg 1801 aagttattag gtgatatcag atccactagt ggcctatgca cccaattcgc cctatagtga 1861 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 1921 cgttacccct gcaggactag tgctgaggca ttaatctact atcggcgact ctctcgaaat 1981 ttttcttaac gcgtccttgt actgcgtcta acgcttttgc cacttggatt tctattatag 2041 gaaatagtct cacttactgg gcgacgaatt ttcgcgtttt gatgaagcac aggaagaatt 2101 tctttttttt ttggcttctt ctggttccgt tttttacgcg cacaaatcta aaaaaagaaa 2161 taattataac ctagtctcga aaattttcat cgatccattc gttccttttt ttcgattttt 2221 tcagatcaaa attcttgttt ctttctttgt cttagtttat attaaaagat attttgattt 2281 tactcctgaa ctatttattc tttctaagaa ggccagaaca ctacagctgt tttaaccgac 2341 tacgaagttc tccattctcg aacactagcc ttcatttacc aaacaggaac tagcgtatat 2401 cattagtcct tattcgaaaa gagattggta gatatttatt gtagtttgtg agaaggagaa 2461 aatactgtca ttggactgat agttagagga cattaacctc tcttacgttc gctcattaag 2521 acctcagcgc ggccgcaaat ttaaataaaa tgaagtgaag ttcctatact ttctagagaa 2581 taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata 2641 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt 2701 ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt 2761 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt 2821 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc 2881 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc 2941 tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac 3001 gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga 3061 ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag 3121 gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct 3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac 3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3961 gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc 4021 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgctttta ccaatgctta atcagtgagg 4081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 4141 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag 4201 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc 4261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 4321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca 4381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 4441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 4501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 4561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 4621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat 4921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4981 tatttgaatg tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa 5041 ttctgaacat tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg 5101 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc 5161 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca 5221 aataaaacga aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt 5281 cgcccttatt cgactctata gtgaagttcc tattctctag aaagtatagg aacttctgaa 5341 gtggggattt aaatgcggcc gcgctgaggg tttaatcggc atgcaaacat ctacacaatt 5401 agcaagggca atccatattt tgtcttttcg cgccctggaa aggcctaagt aatgtcgtaa 5461 acgcattcta tctgtacttc aactctcctc tgtgcattgg tttgtgcaaa tcacatttta 5521 cgatactgcc agatatatgc aaaaagagaa aaccaaggga ccagaacaaa gcaaaattac 5581 gatattcttc gaattccttc gtgcttgact aagacaaagg gatggacgta gcgattttta 5641 gcgggccaag aactggttcc gaaaaagcac aggtacaccg aaccctcagc taaggaggga 5701 cagcaccgat gcggaaggac aaactttctt tttgcctatc acagtatctt atcgagctaa 5761 ctattttcga cacacatgaa aaagcagaaa tattaacgaa aaagaaaaga aagaccatgt 5821 catgtacggg caatcagaat ctgtaacaag cgccattttt ttttctgtat cgggccctcc 5881 ttactgctct ccttccgtgt aacgcgttat gattaagtcc tcagcgagct cgcatggaat 5941 gcgtgcgatg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt 6001 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac 6061 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt 6121 tagaagtgtc aacaacgtat ctac //