LOCUS pCfB2225\(pXII-2-loxP-KanMXsyn) 6144 bp DNA circular SYN 21-SEP-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS p0120 pXII-2-kanMXsyn.
SOURCE synthetic DNA construct.
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6144)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 7. feb 2014 from SnapGene 2.2.1
http://www.snapgene.com
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
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COMMENT VNTDATE|745597978|
COMMENT VNTDBDATE|745598047|
COMMENT LSOWNER|
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COMMENT VNTAUTHORNAME|Irina Borodina|
COMMENT VNTOAUTHORNAME|UNKNOWN|
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FEATURES Location/Qualifiers
source 3995..3994
/organism="synthetic DNA construct"
/mol_type="other DNA"
/vntifkey="98"
source 227..1839
/organism="PCR template vector pUG6"
/mol_type="other DNA"
/db_xref="taxon:142962"
/vntifkey="98"
/note="derived from Saccharomyces cerevisiae"
source 1107..1130
/organism="PCR template vector pUG6"
/mol_type="other DNA"
/db_xref="taxon:142962"
/vntifkey="98"
/note="derived from Saccharomyces cerevisiae"
CDS complement(4058..4918)
/codon_start=1
/vntifkey="4"
/label=AmpR
/note="AmpR"
misc_feature 5377..5911
/vntifkey="21"
/label=XII-2\UP
/note="XII-2 UP"
terminator 5952..6112
/vntifkey="43"
/label=T\ADH1
/note="T ADH1"
terminator 30..218
/vntifkey="43"
/label=T\CYC1
/note="T CYC1"
misc_feature complement(267..300)
/vntifkey="21"
/label=loxP
/note="loxP loxP;loxP site"
promoter 301..698
/vntifkey="30"
/label=P\TEF
/note="P TEF P TEF;TEF2 promoter from Ashbya gossypii"
gene 699..1508
/gene="kanMX"
/vntifkey="60"
/note="kanMX"
CDS 699..1508
/codon_start=1
/transl_table=11
/gene="kanMX"
/product="geneticin resistance protein"
/db_xref="GI:11344910"
/protein_id="AAG34543.1"
/vntifkey="4"
/label=kanMX
/note="kanMX kanMX;Tn903; selection phenotype G418R"
gene 1107..1130
/gene="kanMX"
/vntifkey="60"
/note="kanMX"
terminator 1509..1773
/vntifkey="43"
/label=T\TEF
/note="T TEF T TEF;TEF2terminator from Ashbya gossypii"
misc_feature complement(1774..1807)
/vntifkey="21"
/label=loxP
/note="loxP loxP;loxP site"
misc_feature 1956..2514
/vntifkey="21"
/label=XII-2\Down
/note="XII-2 Down"
rep_origin 3291..3956
/direction=RIGHT
/vntifkey="33"
/label=pUC\ori
/note="pUC ori"
misc_feature 1..29
/vntifkey="21"
/label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT 1665 a 1387 c 1320 g 1772 t
ORIGIN
1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga
61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg
241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat
301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
721 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
781 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
841 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
901 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
961 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
1021 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
1081 ggttacattc gattcctgtt tgtaattgtc cttttaacag cgaccgtgta tttcgtctcg
1141 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
1201 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
1261 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
1321 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
1381 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
1441 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
1501 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
1561 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
1621 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
1681 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
1741 agtgtcgaaa acgagctctc gagaaccctt aatataactt cgtataatgt atgctatacg
1801 aagttattag gtgatatcag atccactagt ggcctatgca cccaattcgc cctatagtga
1861 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
1921 cgttacccct gcaggactag tgctgaggca ttaatctact atcggcgact ctctcgaaat
1981 ttttcttaac gcgtccttgt actgcgtcta acgcttttgc cacttggatt tctattatag
2041 gaaatagtct cacttactgg gcgacgaatt ttcgcgtttt gatgaagcac aggaagaatt
2101 tctttttttt ttggcttctt ctggttccgt tttttacgcg cacaaatcta aaaaaagaaa
2161 taattataac ctagtctcga aaattttcat cgatccattc gttccttttt ttcgattttt
2221 tcagatcaaa attcttgttt ctttctttgt cttagtttat attaaaagat attttgattt
2281 tactcctgaa ctatttattc tttctaagaa ggccagaaca ctacagctgt tttaaccgac
2341 tacgaagttc tccattctcg aacactagcc ttcatttacc aaacaggaac tagcgtatat
2401 cattagtcct tattcgaaaa gagattggta gatatttatt gtagtttgtg agaaggagaa
2461 aatactgtca ttggactgat agttagagga cattaacctc tcttacgttc gctcattaag
2521 acctcagcgc ggccgcaaat ttaaataaaa tgaagtgaag ttcctatact ttctagagaa
2581 taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata
2641 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt
2701 ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt
2761 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt
2821 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc
2881 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc
2941 tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac
3001 gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga
3061 ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag
3121 gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct
3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac
3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3961 gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc
4021 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgctttta ccaatgctta atcagtgagg
4081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
4141 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag
4201 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc
4261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
4321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca
4381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
4441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
4501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
4561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
4621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
4681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
4741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
4801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
4861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat
4921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
4981 tatttgaatg tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa
5041 ttctgaacat tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg
5101 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
5161 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca
5221 aataaaacga aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt
5281 cgcccttatt cgactctata gtgaagttcc tattctctag aaagtatagg aacttctgaa
5341 gtggggattt aaatgcggcc gcgctgaggg tttaatcggc atgcaaacat ctacacaatt
5401 agcaagggca atccatattt tgtcttttcg cgccctggaa aggcctaagt aatgtcgtaa
5461 acgcattcta tctgtacttc aactctcctc tgtgcattgg tttgtgcaaa tcacatttta
5521 cgatactgcc agatatatgc aaaaagagaa aaccaaggga ccagaacaaa gcaaaattac
5581 gatattcttc gaattccttc gtgcttgact aagacaaagg gatggacgta gcgattttta
5641 gcgggccaag aactggttcc gaaaaagcac aggtacaccg aaccctcagc taaggaggga
5701 cagcaccgat gcggaaggac aaactttctt tttgcctatc acagtatctt atcgagctaa
5761 ctattttcga cacacatgaa aaagcagaaa tattaacgaa aaagaaaaga aagaccatgt
5821 catgtacggg caatcagaat ctgtaacaag cgccattttt ttttctgtat cgggccctcc
5881 ttactgctct ccttccgtgt aacgcgttat gattaagtcc tcagcgagct cgcatggaat
5941 gcgtgcgatg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt
6001 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac
6061 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt
6121 tagaagtgtc aacaacgtat ctac
//