LOCUS       pCfB2225\(pXII-2-loxP-KanMXsyn)        6144 bp    DNA     circular SYN 21-SEP-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    p0120 pXII-2-kanMXsyn.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 7. feb 2014 from SnapGene 2.2.1
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|745597978|
COMMENT     VNTDBDATE|745598047|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2225 (pXII-2-loxP-KanMXsyn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     source          3995..3994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     source          227..1839
                     /organism="PCR template vector pUG6"
                     /mol_type="other DNA"
                     /db_xref="taxon:142962"
                     /vntifkey="98"
                     /note="derived from Saccharomyces cerevisiae"
     source          1107..1130
                     /organism="PCR template vector pUG6"
                     /mol_type="other DNA"
                     /db_xref="taxon:142962"
                     /vntifkey="98"
                     /note="derived from Saccharomyces cerevisiae"
     CDS             complement(4058..4918)
                     /codon_start=1
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR"
     misc_feature    5377..5911
                     /vntifkey="21"
                     /label=XII-2\UP
                     /note="XII-2 UP"
     terminator      5952..6112
                     /vntifkey="43"
                     /label=T\ADH1
                     /note="T ADH1"
     terminator      30..218
                     /vntifkey="43"
                     /label=T\CYC1
                     /note="T CYC1"
     misc_feature    complement(267..300)
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP loxP;loxP site"
     promoter        301..698
                     /vntifkey="30"
                     /label=P\TEF
                     /note="P TEF P TEF;TEF2 promoter from Ashbya gossypii"
     gene            699..1508
                     /gene="kanMX"
                     /vntifkey="60"
                     /note="kanMX"
     CDS             699..1508
                     /codon_start=1
                     /transl_table=11
                     /gene="kanMX"
                     /product="geneticin resistance protein"
                     /db_xref="GI:11344910"
                     /protein_id="AAG34543.1"
                     /vntifkey="4"
                     /label=kanMX
                     /note="kanMX kanMX;Tn903; selection phenotype G418R"
     gene            1107..1130
                     /gene="kanMX"
                     /vntifkey="60"
                     /note="kanMX"
     terminator      1509..1773
                     /vntifkey="43"
                     /label=T\TEF
                     /note="T TEF T TEF;TEF2terminator from Ashbya gossypii"
     misc_feature    complement(1774..1807)
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP loxP;loxP site"
     misc_feature    1956..2514
                     /vntifkey="21"
                     /label=XII-2\Down
                     /note="XII-2 Down"
     rep_origin      3291..3956
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="pUC ori"
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1665 a      1387 c      1320 g      1772 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg 
      721 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 
      781 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 
      841 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 
      901 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 
      961 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat 
     1021 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 
     1081 ggttacattc gattcctgtt tgtaattgtc cttttaacag cgaccgtgta tttcgtctcg 
     1141 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 
     1201 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac 
     1261 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 
     1321 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 
     1381 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 
     1441 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 
     1501 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata 
     1561 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt 
     1621 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt 
     1681 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc 
     1741 agtgtcgaaa acgagctctc gagaaccctt aatataactt cgtataatgt atgctatacg 
     1801 aagttattag gtgatatcag atccactagt ggcctatgca cccaattcgc cctatagtga 
     1861 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     1921 cgttacccct gcaggactag tgctgaggca ttaatctact atcggcgact ctctcgaaat 
     1981 ttttcttaac gcgtccttgt actgcgtcta acgcttttgc cacttggatt tctattatag 
     2041 gaaatagtct cacttactgg gcgacgaatt ttcgcgtttt gatgaagcac aggaagaatt 
     2101 tctttttttt ttggcttctt ctggttccgt tttttacgcg cacaaatcta aaaaaagaaa 
     2161 taattataac ctagtctcga aaattttcat cgatccattc gttccttttt ttcgattttt 
     2221 tcagatcaaa attcttgttt ctttctttgt cttagtttat attaaaagat attttgattt 
     2281 tactcctgaa ctatttattc tttctaagaa ggccagaaca ctacagctgt tttaaccgac 
     2341 tacgaagttc tccattctcg aacactagcc ttcatttacc aaacaggaac tagcgtatat 
     2401 cattagtcct tattcgaaaa gagattggta gatatttatt gtagtttgtg agaaggagaa 
     2461 aatactgtca ttggactgat agttagagga cattaacctc tcttacgttc gctcattaag 
     2521 acctcagcgc ggccgcaaat ttaaataaaa tgaagtgaag ttcctatact ttctagagaa 
     2581 taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata 
     2641 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt 
     2701 ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt 
     2761 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt 
     2821 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc 
     2881 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc 
     2941 tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac 
     3001 gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga 
     3061 ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag 
     3121 gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct 
     3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac 
     3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     3961 gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc 
     4021 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgctttta ccaatgctta atcagtgagg 
     4081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     4141 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag 
     4201 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc 
     4261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     4321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca 
     4381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     4441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     4501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     4561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     4621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     4681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     4741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     4801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     4861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat 
     4921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     4981 tatttgaatg tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa 
     5041 ttctgaacat tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg 
     5101 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc 
     5161 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca 
     5221 aataaaacga aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt 
     5281 cgcccttatt cgactctata gtgaagttcc tattctctag aaagtatagg aacttctgaa 
     5341 gtggggattt aaatgcggcc gcgctgaggg tttaatcggc atgcaaacat ctacacaatt 
     5401 agcaagggca atccatattt tgtcttttcg cgccctggaa aggcctaagt aatgtcgtaa 
     5461 acgcattcta tctgtacttc aactctcctc tgtgcattgg tttgtgcaaa tcacatttta 
     5521 cgatactgcc agatatatgc aaaaagagaa aaccaaggga ccagaacaaa gcaaaattac 
     5581 gatattcttc gaattccttc gtgcttgact aagacaaagg gatggacgta gcgattttta 
     5641 gcgggccaag aactggttcc gaaaaagcac aggtacaccg aaccctcagc taaggaggga 
     5701 cagcaccgat gcggaaggac aaactttctt tttgcctatc acagtatctt atcgagctaa 
     5761 ctattttcga cacacatgaa aaagcagaaa tattaacgaa aaagaaaaga aagaccatgt 
     5821 catgtacggg caatcagaat ctgtaacaag cgccattttt ttttctgtat cgggccctcc 
     5881 ttactgctct ccttccgtgt aacgcgttat gattaagtcc tcagcgagct cgcatggaat 
     5941 gcgtgcgatg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt 
     6001 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac 
     6061 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt 
     6121 tagaagtgtc aacaacgtat ctac 
//
