LOCUS       pCfB2337\(XII-5-loxP-HPHMXsyn)        6399 bp    DNA     circular      1-APR-2014
DEFINITION  PCR product of p2073 (pXII-5_no_BsaI).
KEYWORDS    PCR_PRODUCT.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|692984363|
COMMENT     VNTDBDATE|694086503|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2337 (XII-5-loxP-HPHMXsyn)|
COMMENT     VNTAUTHORNAME|Irina Borodina|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(4394..5254)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    5713..6166
                     /vntifkey="21"
                     /label=XII-5\UP
     terminator      6207..6368
                     /vntifkey="43"
                     /label=T\ADH1
     terminator      30..218
                     /vntifkey="43"
                     /label=T\CYC1
     misc_feature    2175..2850
                     /vntifkey="21"
                     /label=XII-5\Down
     rep_origin      3627..4292
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             1993..2026
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
     misc_feature    1728..1967
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1t
     CDS             699..1727
                     /gene="hph"
                     /product="hygromycin B resistance"
                     /vntifkey="4"
                     /label=hph
     misc_feature    320..698
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /vntifkey="21"
                     /label=TEF1p
     CDS             267..300
                     /gene="loxP"
                     /product="recognition site for Cre recombinase"
                     /vntifkey="4"
                     /label=loxP
     misc_feature    1..29
                     /vntifkey="21"
                     /label=USER\cassette\(AsiSI/Nb.BsmI)
BASE COUNT     1666 a      1485 c      1481 g      1767 t 
ORIGIN
        1 caacggaatg cgtgcgatcg cgtgcattca tccgctctaa ccgaaaagga aggagttaga 
       61 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 
      121 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 
      181 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg 
      241 tacgctgcag gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
      301 taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg 
      361 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac 
      421 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt 
      481 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga 
      541 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata 
      601 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta 
      661 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca 
      721 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 
      781 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 
      841 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact 
      901 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc 
      961 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg 
     1021 aactgcccgc tgttctgcag ccggtcgcgg aggcaatgga tgccattgct gcggccgatc 
     1081 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat 
     1141 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg 
     1201 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg 
     1261 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg 
     1321 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat 
     1381 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc 
     1441 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc 
     1501 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag 
     1561 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta 
     1621 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg 
     1681 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa 
     1741 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt 
     1801 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc 
     1861 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt 
     1921 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctctcg 
     1981 agaaccctta atataacttc gtataatgta tgctatacga agttattagg tgatatcaga 
     2041 tccactagtg gcctatgcac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 
     2101 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccctg caggactagt 
     2161 gctgaggcat taatgcaact cagaagtttg acagcaagca agttcatcat tcgaactagc 
     2221 cttattgttt tagttcagtg acagcgaact gccgtactcg atgctttatt tctcacggta 
     2281 gagcggaaga acagataggg gcagcgtgag aagagttaga aagtaaattt ttatcacgtc 
     2341 tgaagtattc ttattcatag gaaattttgc aaggtttttt agctcaataa cgggctaagt 
     2401 tatataaggt gttcacgcga ttttcttgtt atgtatacct cttctctgag gaatggtact 
     2461 actgtcctga tgtaggctcc ttaaattggt gggcaagaat aacttatcga tattttgtat 
     2521 attggtcttg gagttcacca cgtaatgcct gtttaagacc atcagttaac tctagtatta 
     2581 tttggtcttg gctactggcc gtttgctatt attcaagtct tttgtgcctt cccgtcgggt 
     2641 aagggagtta tttagggata cagaatctaa cgaaaactaa atctcaatga ttaactctat 
     2701 ttaatccttt tttgaaaggc aaaagaggtc ccttgttcac ttacaacgtt cttagccaaa 
     2761 ttcgcttatc acttactact tcacgatata cagaagtaaa aacatataaa aagatgtctg 
     2821 tttgtttagc catcacaaaa ggtatcgcag attaagacct cagcgcggcc gcaaatttaa 
     2881 ataaaatgaa gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa 
     2941 taagggcgac acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt 
     3001 gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc 
     3061 tttattattt tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat 
     3121 acaaaaaatc ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag 
     3181 caacgtatct tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca 
     3241 tatatcaagc aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact 
     3301 ccccccataa aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact 
     3361 cgttatcaga accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga 
     3421 aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc 
     3481 tggcagttcc ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     3541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     3601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     3661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     3721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     3781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     3841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     3901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     3961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     4021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     4081 agagttcttg aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg 
     4141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     4201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     4261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc 
     4321 gcgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg 
     4381 taatgctctg cttttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     4441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     4501 taccatctgg ccccagcgct gcgatgatac cgcgagaacc acgctcaccg gctccggatt 
     4561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     4621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     4681 atagtttgcg caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     4741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     4801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     4861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     4921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     4981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     5041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     5101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     5161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     5221 gaataagggc gacacggaaa tgttgaatac tcatattctt cctttttcaa tattattgaa 
     5281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     5341 aacaaatagg ggtcagtgtt acaaccaatt aaccaattct gaacattatc gcgagcccat 
     5401 ttatacctga atatggctca taacacccct tgtttgcctg gcggcagtag cgcggtggtc 
     5461 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg 
     5521 actccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 
     5581 agactgggcc tttcgcccgg gctaattatg gggtgtcgcc cttattcgac tctatagtga 
     5641 agttcctatt ctctagaaag tataggaact tctgaagtgg ggatttaaat gcggccgcgc 
     5701 tgagggttta atcaatctgg cggcttgagt tctcaacatg ttttattttt tacttatatt 
     5761 gctggtaggg taaaaaaata taactcctag gaataggttg tctatatgtt tttgtcttgc 
     5821 ttctataatt gtaacaaaca aggaaaggga aaatactggg tgtaaaagcc attgagtcaa 
     5881 gttaggtcat cccttttata caaaattttt caattttttt tccaagattc ttgtacgatt 
     5941 aattattttt tttttgcgtc ctacagcgtg atgaaaattt cgcctgctgc aagatgagcg 
     6001 ggaacgggcg aaatgtgcac gcgcacaact tacgaaacgc ggatgagtca ctgacagcca 
     6061 ccgcagaggt tctgactcct actgagctct attggaggtg gcagaaccgg taccggagga 
     6121 ggccgctata accggtttga atttattgtc acagtgtcac atcagcatta agtcctcagc 
     6181 gagctcgcat ggaatgcgtg cgatgagcga cctcatgcta tacctgagaa agcaacctga 
     6241 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 
     6301 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 
     6361 agaaattcgc ttatttagaa gtgtcaacaa cgtatctac 
//
