LOCUS       Exported                4374 bp ds-DNA     circular SYN 22-OCT-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    1449 pmE-6MTnlstdTomato2A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 22, 2015 from SnapGene 2.8.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4374
     primer_bind     536..553
                     /label=M13-fwd
                     /note="M13-fwd"
     misc_recomb     570..668
                     /label=AttL1
                     /note="AttL1"
     misc_feature    616
                     /note="differs from normal attL1.  confirmed 10/22/2015. 
                     Does not affect LR rxn"
     misc_feature    669..676
                     /note="kozak"
     misc_feature    704..742
                     /note="myc tag 1"
     misc_feature    743..781
                     /note="myc tag 2"
     misc_feature    782..820
                     /note="myc tag 3"
     misc_feature    821..859
                     /note="myc tag 4"
     misc_feature    860..898
                     /note="myc tag 5"
     misc_feature    923..964
                     /note="myc tag 6"
     misc_feature    980..1000
                     /note="nuclear localization signal"
     misc_feature    1001..2428
                     /note="tdTomato (2X)"
     misc_feature    2432..2497
                     /note="viral 2A peptide"
     misc_recomb     complement(2498..2597)
                     /label=AttL2
                     /note="AttL2"
     primer_bind     complement(2615..2636)
                     /label=T7
                     /note="T7"
     primer_bind     complement(2635..2655)
                     /label=M13-rev
                     /note="M13-rev"
     CDS             2768..3574
                     /label=KanR
                     /note="KanR"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gccaccatgg ctggatccca tcgatttaaa gctatggagc aaaagctcat
      721 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      781 aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc
      841 tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat
      901 ggagagcttg ggcgacctca ccatggagca aaagctcatt tctgaagagg acttgaattt
      961 agagatctcg aattccatgc ccaagaagaa gaggaaggtg atggtgagca agggcgagga
     1021 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
     1081 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1141 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
     1201 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1261 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1321 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1381 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1441 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1501 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1561 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1621 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
     1681 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa
     1741 catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     1801 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1861 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     1921 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     1981 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     2041 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     2101 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2161 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     2221 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     2281 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     2341 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     2401 cctgtacggc atggacgagc tgtacaaggg aggatccgga gccacgaact tctctctgtt
     2461 aaagcaagca ggagacgtgg aagaaaaccc cggtcctcac ccagctttct tgtacaaagt
     2521 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2581 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2641 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2701 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2761 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2821 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2881 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2941 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3001 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3061 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3121 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3181 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3241 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3301 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3361 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3421 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3481 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3541 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3601 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3661 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3721 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3781 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3841 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3901 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3961 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4021 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4081 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4141 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4201 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4261 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4321 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
