LOCUS       pTol1-UAS-nlstdT        5756 bp    DNA     linear       25-JUN-2015
FEATURES             Location/Qualifiers
     misc_feature    3472..3500
                     /note="29 bp repeated pUC57 sequence"
     misc_feature    3501..3523
                     /note="unknown sequence"
     misc_feature    3524..3552
                     /note="29 bp repeated sequence"
     source          402..3388
                     /dnas_title="pTol1-UAS-nlstdTomato"
                     /organism="Oryzias latipes"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:8090"
     mobile_element  402..3388
                     /mobile_element_type="transposon:Tol-tyr"
     repeat_region   402..423
                     /standard_name="terminal inverted repeat"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="terminal repeat; terminal repeat of Tol-tyr"
                     /citation=[1]
                     /rpt_type=inverted
     misc_feature    410..566
                     /note="Tol1 left arm"
     misc_feature    628..648
                     /note="attR4"
     misc_feature    3080..3275
                     /note="SV40 UTR/pA from pCS2"
     misc_feature    3038..3058
                     /note="attR3"
     repeat_region   3375..3388
                     /standard_name="terminal inverted repeat"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="terminal repeat of Tol-tyr"
                     /citation=[1]
                     /rpt_type=inverted
     misc_feature    3283..3388
                     /note="Tol1 right arm"
     misc_feature    1133..1156
                     /note="attB1"
     misc_feature    2987..3011
                     /note="attB2"
     misc_feature    731..747
                     /note=""
     misc_feature    754..770
                     /note="UAS"
     misc_feature    778..794
                     /note="UAS"
     misc_feature    806..822
                     /note="UAS"
     misc_feature    831..847
                     /note="UAS"
     misc_feature    854..870
                     /note="UAS"
     misc_feature    878..894
                     /note="UAS"
     misc_feature    906..922
                     /note="UAS"
     misc_feature    931..947
                     /note="UAS"
     misc_feature    954..970
                     /note="UAS"
     misc_feature    983..1041
                     /note="E1B basal promoter"
     misc_feature    1157..1164
                     /note="kozak"
     misc_feature    1192..1230
                     /note="myc tag 1"
     misc_feature    1231..1269
                     /note="myc tag 2"
     misc_feature    1270..1308
                     /note="myc tag 3"
     misc_feature    1309..1347
                     /note="myc tag 4"
     misc_feature    1348..1386
                     /note="myc tag 5"
     misc_feature    1411..1452
                     /note="myc tag 6"
     misc_feature    1157..1194
                     /note="4697-attB1-kozak-pCS-2F"
     misc_feature    1465..1488
                     /note=""
     misc_feature    1468..1488
                     /note="nuclear localization signal"
     misc_feature    1489..2916
                     /note="tdTomato (2X)"
     misc_feature    complement(1495..1514)
                     /note="4276-tomatoREV"
     misc_feature    complement(2892..2985)
                     /note="4738-attB2-mCherry/tdTomato-2A-R"
     misc_feature    2920..2985
                     /note="viral 2A peptide"
     misc_feature    2890..2913
                     /note="4855-tdTomato-3prime-F"
     misc_feature    3012..3019
                     /note="AscI "
     misc_feature    3020..3025
                     /note="SnaBI"
     misc_feature    3026..3031
                     /note="SpeI"
     misc_feature    3032..3037
                     /note="XhoI"
ORIGIN      
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCCTTTAGCc agtagcggtt
      421 ctaggcacgg gccgtccggg cggtggcctg gggcggaaaa ctgaaggggg gcggcaccgg
      481 cggctcagcc ctttgtaata tattaatatg caccactatt ggtttactta tgtcacagtt
      541 tgtaagtttg taacagcctg aacctggccg cgcgccattg ggatggaacc ctcgaggggc
      601 ccgctagcga tatccctgca gggctatcaa ctttgtatag aaaagttgGT AATACGACTC
      661 ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTAG
      721 GCCTCCAAGG CGGAGTACTG TCCTCCGGGC TGGCGGAGTA CTGTCCTCCG GCAAGGTCGG
      781 AGTACTGTCC TCCGACACTA GAGGTCGGAG TACTGTCCTC CGACGCAAGG CGGAGTACTG
      841 TCCTCCGGGC TXGCGGAGTA CTGTCCTCCG GCAAGGTCGG AGTACTGTCC TCCGACACTA
      901 GAGGTCGGAG TACTGTCCTC CGACGCAAGG CGGAGTACTG TCCTCCGGGC TGGCGGAGTA
      961 CTGTCCTCCG GCAAGGGTCG ACTCTAGAGG GTATATAATG GATCCCATCG CGTCTCAGCC
     1021 TCACTTTGAG CTCCTCCACA CGAATTCCTG CAGCCCGGGG GATCCACTAG TTCTAGAGCG
     1081 GCCGCCACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTcaagtttg
     1141 tacaaaaaag caggctCCGC CACCATGGCt GGATCCCATC GATTtaaagc tatggagcaa
     1201 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
     1261 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
     1321 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
     1381 aatgaaatgg agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac
     1441 ttgaatttag agatctcGAA TTCcATGCCC AAGAAGAAGA GGAAGGtgat ggtgagcaag
     1501 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1561 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1621 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1681 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     1741 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1801 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1861 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1921 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1981 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2041 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2101 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2161 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     2221 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     2281 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2341 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     2401 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     2461 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2521 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     2581 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2641 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     2701 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     2761 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2821 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2881 cacctgttcc tgtacggcat ggacgagctg tacaagggAG GATCCGGAGC CACGAACTTC
     2941 TCTCTGTTAA AGCAAGCAGG AGACGTGGAA GAAAACCCCG GTCCTCcacc cagctttctt
     3001 gtacaaagtg gGGCGCGCCT ACGTAACTAG TCTCGAGcaa ctttattata catagttgat
     3061 aaGGCGCGCC AGGCCTGTAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC
     3121 CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
     3181 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
     3241 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAATTa aaacatttca gttgacgaag
     3301 acaaacaaag ttctgttgtg actatggggg gggggggcgc ctggggatgg tctcgcccgg
     3361 ggagtaattc agggtagaac cgccactgGC TAAAGGGAGC TCGGTACCTC GCGAATGCAT
     3421 CTAGATATCG GATCCCGGGC CCGTCGACTG CAGAGGCCTG CATGCaagct tGCGTAATCA
     3481 TGGTCATAGC TGTTTCCTGT ATCCCAATGG CGCGCCGAGC TTGGCGTAAT CATGGTCATA
     3541 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
     3601 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
     3661 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
     3721 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     3781 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     3841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     3901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     3961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     4021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     4081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     4141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     4201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     4261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     4321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     4381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     4441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     4501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     4561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     4621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     4681 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     4741 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     4801 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     4861 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     4921 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     4981 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     5041 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     5101 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     5161 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     5221 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     5281 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     5341 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     5401 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     5461 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     5521 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     5581 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     5641 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC
     5701 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC
//

